Effects of Storage Condition on the Bioactive Compound Contents of Korean Cabbage

다국어 초록 (Multilingual Abstract)

The influence of common storage condition (i.e., temperature and period) on the phytochemical contents of Korean cabbage was investigated. The total polyphenol and flavonoid contents of fresh Korean cabbage extract were 308.48 μg gallic acid equivalent/g d.w. and 5.33 μg quercetin equivalent/g d.w., respectively. Seven glucosinolate peaks were detected in Korean cabbage representing progoitrin, glucoalyssin, gluconapin, glucobrassicanapin,glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin based on HPLC and LC-MS analysis. The concentration of total glucosinolates in fresh Korean cabbage was 13.55 μmol/g d.w. Our results suggest that the total glucosinolate content in Korean cabbage did not much change more over 7 days of storage at 4oC but a little bit decreased in stored at ambient temperature. The extractable β-carotene, lutein as well as tocopherol contents in Korean cabbage were decreased by increasing storage period at both temperatures of 4oC and ambient temperature. Longer storage period at ambient temperature may reduce the levels of health promoting compounds. Based on our results refrigeration at 4oC can help preserve the nutritive value of Korean cabbage by maintaining high levels of glucosinolates and other bioactive compounds.

참고문헌 (Reference)

1 Bones AM, "The myrosinase-glucosinolate system, its organization and biochemistry" 97 : 194-208, 1996

2 Rangkadilok N, "The effect of post-harvest and packaging treatments on glucoraphanin concentration in broccoli (Brassica oleracea var. italica)" 50 : 7386-7391, 2002

3 Stacewicz-Sapuntzakis M, "Simultaneous determination of serum retinol and various carotenoids: Their distribution in middle aged men and women" 3 : 27-45, 1987

4 ISO, "Rapeseed: Determination of glucosinolates content - Part 1: Method using high performance liquid chromatography. 9167-1: 1992(E)"

5 Davey MW, "Plant Lascorbic acid: Chemistry, function, metabolism, bioavailability, and effects of processing" 80 : 825-860, 2000

6 Hwang E-S, "Phenylethyl isothiocyanate and its Nacetylcysteine conjugate suppress the metastasis of SK-Hep1 human hepatoma cells" 17 : 837-846, 2006

7 Vallejo F, "Health-promoting compounds in broccoli as influenced by refrigerated transport and retail sale period" 51 : 3029-3034, 2003

8 Traka M, "Glucosinolates, isothiocyanates, and human health" 8 : 269-282, 2009

9 Hansen M, "Glucosinolates in broccoli stored under controlled atmosphere" 120 : 1069-1074, 1995

10 Fenwick GR, "Glucosinolates and their breakdown products in food and food plants" 18 : 123-201, 1983

11 Farnham MW, "Genetic and environmental effects on glucosinolate content and chemoprotective potency of broccoli" 123 : 60-65, 2004

12 Hwang E-S, "Evaluation of urinary N-acetyl cysteinyl allylisothiocyanate as a biomarker for intake and bioactivity of Brussels sprouts" 41 : 1817-1825, 2003

13 Verkert R, "Effects of processing conditions on glucosinolates in cruciferous vegetables" 114 : 193-194, 1997

14 Song L, "Effect of storage, processing, and cooking on glucosinolate content of Brassica vegetables" 45 : 216-224, 2007

15 Jia CG, "Effect of modified atmosphere packaging on visual quality and glucosinolates of broccoli florets" 114 : 28-37, 2009

16 Shapiro TA, "Chemopreventive glucosinolates and isothiocyanates of broccoli sprouts: Metabolism and excretion in humans" 10 : 501-508, 2002

17 Singleton VL, "Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin- Ciocalteu reagent" 299 : 152-178, 1999

18 Woisky RG, "Analysis of propolis: Some parameters and procedures for chemical quality control" 37 : 99-105, 1998

19 Rabot S, "Alterations of the hepatic xenobiotic-metabolizing enzymes by a glucosinolate-rich diet in germ-free rats: Influence of a pre-induction with phenobarbital" 70 : 347-354, 1993

20 Zhang Y, "A major inducer of anticarcinogenic protective enzymes from broccoli: Isolation and elucidation of structure" 89 : 2399-2403, 1992