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    Penicillium屬이 生産하는 alkaline protease에 關하여

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    https://www.riss.kr/link?id=A76056275

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    The alkaline protease was isolated from the culture of Penicillium species (P-46) grown in the wheat bran media. The crude purification of this enzyme was carried out by extraction with distilled water and precipitated with 0.7~saturated ammonium sulfate, then dialysis for 3days.<br/>
    The activity of this enzyme was determined by Folin's colorimetric method. The results were as follows;<br/>
    1. The optimum pH and temperature of this enzyme were pH 8.4 and 45℃.<br/>
    2. This enzyme was stable at pH 7.0~9.0.<br/>
    3. This enzyme was not inactivated by treatment in lower temperature than 30℃.<br/>
    4. The activity of this enzyme was strongly inhibited by Hg^(++) and Cu^(++), but slightly by Ag<br/>
    5. This enzyme was not inhibited by cystein, thiourea, ε-aminocaproic acid, 2. 4-DNP. EDTA but strongly inhibited by PCMB.
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    The alkaline protease was isolated from the culture of Penicillium species (P-46) grown in the wheat bran media. The crude purification of this enzyme was carried out by extraction with distilled water and precipitated with 0.7~saturated ammonium sulf...

    The alkaline protease was isolated from the culture of Penicillium species (P-46) grown in the wheat bran media. The crude purification of this enzyme was carried out by extraction with distilled water and precipitated with 0.7~saturated ammonium sulfate, then dialysis for 3days.<br/>
    The activity of this enzyme was determined by Folin's colorimetric method. The results were as follows;<br/>
    1. The optimum pH and temperature of this enzyme were pH 8.4 and 45℃.<br/>
    2. This enzyme was stable at pH 7.0~9.0.<br/>
    3. This enzyme was not inactivated by treatment in lower temperature than 30℃.<br/>
    4. The activity of this enzyme was strongly inhibited by Hg^(++) and Cu^(++), but slightly by Ag<br/>
    5. This enzyme was not inhibited by cystein, thiourea, ε-aminocaproic acid, 2. 4-DNP. EDTA but strongly inhibited by PCMB.

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    목차 (Table of Contents)

    • Abstract
    • 緖論
    • 實驗材料 및 方法
    • 實驗結果
    • 考察
    • Abstract
    • 緖論
    • 實驗材料 및 方法
    • 實驗結果
    • 考察
    • 結論
    • 參考文獻
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