The alkaline protease was isolated from the culture of Penicillium species (P-46) grown in the wheat bran media. The crude purification of this enzyme was carried out by extraction with distilled water and precipitated with 0.7~saturated ammonium sulf...
The alkaline protease was isolated from the culture of Penicillium species (P-46) grown in the wheat bran media. The crude purification of this enzyme was carried out by extraction with distilled water and precipitated with 0.7~saturated ammonium sulfate, then dialysis for 3days.<br/>
The activity of this enzyme was determined by Folin's colorimetric method. The results were as follows;<br/>
1. The optimum pH and temperature of this enzyme were pH 8.4 and 45℃.<br/>
2. This enzyme was stable at pH 7.0~9.0.<br/>
3. This enzyme was not inactivated by treatment in lower temperature than 30℃.<br/>
4. The activity of this enzyme was strongly inhibited by Hg^(++) and Cu^(++), but slightly by Ag<br/>
5. This enzyme was not inhibited by cystein, thiourea, ε-aminocaproic acid, 2. 4-DNP. EDTA but strongly inhibited by PCMB.