From the hyperalbuminemia patient who had overexpressed albumin, his genomic DNA was isolated and its albumin gene promoter region was amplified by using PCR and subcloned into pTZ 19U vector. The longer the extension time was the more product was yie...
From the hyperalbuminemia patient who had overexpressed albumin, his genomic DNA was isolated and its albumin gene promoter region was amplified by using PCR and subcloned into pTZ 19U vector. The longer the extension time was the more product was yielded. Specificity was increased when higher annealing temperature and less concentration of two primers were used. Based on RNA slot blotting the patient`s albumin gene should be expressed ten-fold higher than that of the normal. We sequenced 600 base pair of human albumin promotor region and did not find any mutation. Thus the hyperalbuminemia might result from the mutation on the transcription factors of albumin promoter.