실규모 하수처리 생물반응기에서 발견되는 암모니아산화균 군집조성 및 특징 = Characterization and Composition of Ammonia-Oxidizing Bacterial Community in Full- Scale Wastewater Treatment Bioreactors

다국어 초록 (Multilingual Abstract)

Ammonia-oxidizing bacteria (AOB) are chemolithoautotrophs that play a key role in nitrogen removal from advanced wastewater treatment processes. Various AOB species inhabit and their community compositions vary over time in the wastewater treatment bioreactors. In this study, a hypothesis that operational and environmental conditions affect both the community compositions and the diversity of AOB in the bioreactors was proposed. To verify the hypothesis, the clone libraries based on ammonia monooxygenase subunit A were constructed using activated sludge samples from aerobic bioreactors at the Pohang, the Palo Alto, the Nine Springs, and the Marshall wastewater treatment plants (WWTPs). In those bioreactors, AOB within the Nitrosomonas europaea, N. oligotropha, N.-like, and Nitrosospira lineages were commonly found, while AOB within the N. communis, N. marina, and N. cryotolerans lineages were rarely detected in the samples. The AOB community structures were different in the bioreactors: AOB within the N. oligotropha lineage were the major microorganisms in the Pohang, the Palo Alto, and the Marshall WWTPs, while AOB within the N. europaea lineage were dominant in the Nine Springs WWTP. The correlations between the AOB community compositions of the wastewater treatment bioreactors and their operational (HRT, SRT, and MLSS) and environmental conditions (temperature, pH, COD, $NH_3$, and $NO_3{^-}$) were evaluated using a multivariate statistical analysis called the Redundancy Analysis (RDA). As a result, COD and $NO_3{^-}$ concentrations in the bioreactors were the statistically significant variables influencing the AOB community structures in the wastewater treatment bioreactors.

참고문헌 (Reference)

1 이정수, "하·폐수처리: 최신 이론 및 응용" 도서출판 동화기술 2005

2 Metcalf and Eddy, "Wastewater engineering: treatment and reuse. 4th ed" McGraw-Hill 2003

3 Saitou, N, "The neighbor-joining method - a new method for reconstructing phylogenetic trees" 4 : 406-425, 1987

4 Rotthauwe, J.H., "The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations" 63 : 4704-4712, 1997

5 Thompson, J.D., "The Clustal_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools" 25 : 4876-4882, 1997

6 Park, H.D., "Taking advantage of aerated-anoxic operation in a full-scale University of Cape Town (UCT) process" 78 : 637-642, 2006

7 APHA, "Standard methods for the examination of water and wastewater. 17th ed." APHA, AWWA, WPCF 1989

8 Park, H.D., "Redundancy analysis demonstrated the relevance of temperature to ammonia-oxidizing bacterial community compositions in a full-scale nitrifying biore actor treating saline wastewater" J. Microbiol. Biotechnol. 2008

9 Limpiyakorn, T., "Quantification of ammonia-oxidizing bacteria populations in full-scale sewage activated sludge systems and assessment of system variables affecting their performance" 54 : 91-99, 2006

10 Dionisi, H.M., "Quantification of Nitrosomonas oligotropha- like ammonia-oxidizing bacteria and Nitrospira spp. from full-scale wastewater treatment plants by competitive PCR" 68 : 245-253, 2002

11 Purkhold, U., "Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys" 66 : 5368-5382, 2000

12 Watson, S.W., "Nitrifying bacteria. in Bergey's Manual of Systematic Bacteriology" Williams & Wilkins 1834

13 Park, H.-D., "Molecular analysis of ammonia-oxidizing bacterial populations in aerated-anoxic Orbal processes" 46 : 273-280, 2002

14 US-EPA, "Manual: Nitrogen control" USEPA 1993

15 Koops, H.P, "Distribution and ecophysiology of the nitrifying bacteria emphasizing cultured species" 37 : 1-9, 2001

16 Limpiyakorn, T., "Communities of ammonia-oxidizing bacteria in activated sludge of various sewage treatment plants in Tokyo" 54 : 205-217, 2005

17 Wagner, M., "Combining fluorescent in situ hybridization (FISH) with cultivation and mathematical modeling to study population structure and function of ammonia-oxidizing bacteria in activated sludge" 37 : 441-449, 1998

18 Koops, H.P., "Classification of eight new species of ammoniaoxidizing bacteria: Nitrosomonas communis sp. nov., Nitrosomonas urea sp. nov., Nitrosomonas aestuarii sp. nov., Nitrosomonas marina sp. nov., Nitrosomonas nitrosa sp. nov., Nitrosomonas eutropha sp. nov., Nitrosomonas oligotropha sp. nov. and Nitrosomonas halophila sp" 137 : 1689-1699, 1991

19 ter Braak,C.J.F, "Canonical correspondence analysis: a new eigenvector technique for multivariate direct gradient analysis" 67 : 1167-1179, 1986

20 McCune, B, "Analysis of ecological communities" Gleneden Beach, OR, MJM Software Design 2002

21 Hiorns, W.D., "Amplification of 16S ribosomal RNA genes of autotrophic ammonia-oxidizing bacteria demonstrates the ubiquity of nitrosospiras in the environment" 141 : 2793-2800, 1995

22 Kowalchuk, G.A, "Ammonia-oxidizing bacteria: A model for molecular microbial ecology" 55 : 485-529, 2001