가토 결막세포내에서의 Chlamydia trachomatis의 구조에 관한 전자현미경적 연구 = Electron Microscopic Studies on the Structure of Chlamydia trachomatis in the Cell of Rabbit Conjunctiva

다국어 초록 (Multilingual Abstract)

As human pathogens, the organism of C. trachomatis cause a number of diseases. Primarily these are diseases of the eye (tranchoma and inclusion conjunctivitis) and of the urogenital tract (lymphogranuloma venerum (LGV), urethritis, and cervicititis). The Chlamydiae are non motile, gram negative organism that sharing a similar morphology, and a common group antigen. They og through a unique developmental cycle within the cytoplasm of their host's cells. The Chlamydiae are prokaryotic obligate parasites of eukaryotic cells. Chlamydiae have two morphological forms-elementary body(EB) and the reticulate body(RB).
We studied the ultrastructure of Chlamydia trachomatis in the rabbit's conjunctiva using electron microscope (Hitachi H-600). The segments of conjunctiva were cultured in the Eaglis modified essential medium containing 10% fetal calf serum before inoculation of elementary bodies.
The results were as follows;
1. The purified elementary body was uniformly spherical particel containing electron-dense material and covered with two lamellated membranes of about 0.3μdiameter.
2. After 12 hrs of infection, elementary bodies were phagocytosed by endocytosis. The elementary body was enclosed by a vacuole made from the cell membrane.
3. After 24 hrs of infection, elementary bodies were changing to reticulate bodies which began to undergo binary fission.
4. After 48 hrs of infection, there were complete changes from elementary bodies to reticulate bodies and numerous glycogen particles in the cytoplasm of cell.
5. After 72 hrs of infection, there were complete of reticulate bodies to elementary bodies and increased number and size of vesicles in cytoplasm of cell.