색소 생성균 Bacillus sp. DC-2 를 이용한 Protease 생성 = Protease Production Using by Pigment - producing Bacterium Bacterium Bacillus sp.DC-2

다국어 초록 (Multilingual Abstract)

The strain DC-2, which was isolated from 42 Korean traditional fermented soybean products was tested to pigment production ability and protease activity. In the pigment production ability, the more concentration of the soybean, the more pigmentation was obtained. This strain was identified Bacillus sp. It was confirmed that optimum conditions for culture media were 0.5% of maltose, 0.25% of ammonium Sulfate, 0.5% of yeast extract, 0.1% of MgSO₄ ? 7H₂O, and 1.0% of NaCl per distilled water. The high efficient condition of culture was obtained at pH 7.0 and 37 It was examined that optimum conditions for protease production media were 0.5% of maltose, 0.25% of ammonium sulfate, 0.5% of yeast extract, 0.3% of K₂HPO₄, and 0.01% of MgSO₄ ? 7H₂O per distilled water. The best efficient condition of protease activity was obtained at pH 7.5 and 37℃ . The strain was exhibited strong resistance against metal salts, Na₂HAsO₄, Na₂MoO₄ ? 2H₂O and LiCl₂ and antibiotic, chloramphenicol.