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      溫度調節型 發現 Vector를 함유한 Phenylalanine 生産菌의 分子有種 = Molecular Breeding of Phenylalanine Producing E.coli Containing Temperature - Controllable Vector

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      https://www.riss.kr/link?id=A2004491

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      다국어 초록 (Multilingual Abstract)

      In order to produce phenylalanine without tyrosine co-prodction, we constructed various temperature-controllable expression vectors by insertion of lower expression of the tyrA gene into plasmid pSY 130-14.
      And tyrosine revertant to cultivate without addition of tyrosine, was selected from Escherichia coli strain AT2471[tyrA­,thi­]by spontaneous mutation,
      The strain AT2471 harbouring plasmid pSY 146A and the tyrosine revertant 5 harbouring plasmid pSY 111-14 produced 12g/ℓand 15g/ℓof phenylalanine respectively in 2.5ℓjar fermenter at a constant temperature of 39℃ after 55 hours cultivation.





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      In order to produce phenylalanine without tyrosine co-prodction, we constructed various temperature-controllable expression vectors by insertion of lower expression of the tyrA gene into plasmid pSY 130-14. And tyrosine revertant to cultivate withou...

      In order to produce phenylalanine without tyrosine co-prodction, we constructed various temperature-controllable expression vectors by insertion of lower expression of the tyrA gene into plasmid pSY 130-14.
      And tyrosine revertant to cultivate without addition of tyrosine, was selected from Escherichia coli strain AT2471[tyrA­,thi­]by spontaneous mutation,
      The strain AT2471 harbouring plasmid pSY 146A and the tyrosine revertant 5 harbouring plasmid pSY 111-14 produced 12g/ℓand 15g/ℓof phenylalanine respectively in 2.5ℓjar fermenter at a constant temperature of 39℃ after 55 hours cultivation.





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