HL-60 세포 분화유도증 c-Myc 발현에 대한 세포분화유도제의 영향 = Effects of the Differentiation Inducers on Regulation of c-Myc Gene Expression during Differentiation of Human Promyelocytic Leukemia Cell Line HL-60

다국어 초록 (Multilingual Abstract)

Effects of the differentiation inducers on DNA synthesis and c-myc gene expression have been investigated during the differentiation of human promyelocytic leukemia cell line HL-60.
All-trans retinoic acid(retinoic acid) and dimethyl sulfoxide(DMSO) decreased DNA synthesis at 24 hours and 12-O-tetradecanoylphorbol 13-acetate(TPA) decreased at 12 hours after exposure, respectively. On the other hand control, no adding differentiation inducers, gradually increased DNA synthesis. The c-myc mRNA was sharply reduced at 24 hours in retinoic acid-, at 4 hours in DMSO-, and at 1 hour in TPA-exposured HL- 60 cells. The level of c-myc mRNAs was reduced in proportion to the concentration in all of three differentiation inducers. The level of c-myc mRNAs in the differentiation inducers-exposured cells were elevated by cycloheximide treatment.
These results suggest that c-myc gene expression and differentiation mechanism are variable according to the kinds of the differentiation inducers in HL-60 cells.