Overexpression of OsmiR393a gene confers drought tolerance in creeping bentgrass

다국어 초록 (Multilingual Abstract)

Drought is a relatively important abiotic stress of creeping bentgrass (Agrostis stolonifera L.). Limited information is available with regard to molecular management strategies for drought. MiR393a is already known to play role in the tolerance of plants to abiotic stresses. Here, creeping bentgrass plants overexpressing OsmiR393a showed improved drought tolerance, as evidenced by maintenance of turgor and increased growth. Additional analyses revealed that the transgenic plants generally displayed lower transpiration rate, higher proline content and chlorophyll content when subjected to drought stress. These results suggest OsmiR393a may improve drought tolerance of creeping bentgrass by regulating 2, 4- D signalling and other pathways in response to the stress conditions.

참고문헌 (Reference)

1 Anna M, "miR393 contributes to the embryogenic transition induced in vitro in Arabidopsis via the modification of the tissue sensitivity to auxin treatment" 244 : 231-243, 2016

2 Chail ML, "Transgenic plants of colonial bentgrass from embryogenic callus via Agrobacterium-mediated transformation" 77 : 165-171, 2004

3 Cao MX, "Transformation of recalcitrant turfgrass cultivars through improvement of tissue culture and selection regime" 85 : 307-316, 2006

4 Abdeen A, "Transcriptome analysis reveals absence of unintended effects in drought-tolerant transgenic plants overexpressing the transcription factor ABF3" 11 : 69-, 2010

5 Yamaguchi-Shinozaki K, "Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses" 57 : 781-803, 2006

6 Cortina C, "Tomato abiotic stress enhanced tolerance by trehalose biosynthesis" 169 : 75-82, 2005

7 Abebe T, "Tolerance of mannitol-accumulating transgenic wheat to water stress and salinity" 131 : 1748-1755, 2003

8 Mano Y, "The pathway of auxin biosynthesis in plants" 63 : 2853-2872, 2012

9 Haksong Pak Y, "The effect of exogenous methyl jasmonate on the flowering time, floral organ morphology, and transcript levels of a group of genes implicated in the development of oilseed rape flowers(Brassica napus L. )" 231 : 79-91, 2009

10 Ha CV, "The auxin response factor transcription factor family in soybean : genome-wide identification and expression analyses during development and water stress" 10 : 1093-, 2013

11 Reguera M, "Targeting, metabolic pathways for genetic engineering abiotic stress-tolerance in crops" 1819 : 186-194, 2012

12 Vidal EA, "Systems approaches map regulatory networks downstream of the auxin receptor AFB3 in the nitrate response of Arabidopsis thaliana roots" 110 : 12840-12845, 2013

13 Bajaj S, "Ryegrass genes—a potential source for improving rice and grass species" 18 : 71-73, 2010

14 Shinozaki K, "Regulatory network of gene expression in the drought and cold stress responses" 6 : 410-417, 2003

15 Kindiger M, "Registration of ‘Nanryo’ tall fescue" 46 : 1815-1816, 2006

16 Badu-Apraku B, "Registration of striga-resistant and drought-tolerant tropical early maize populations TZE-W Pop DT STR C4 and TZE-Y Pop DT STR C4" 3 : 86-90, 2009

17 Zhang Y, "Recent molecular and genomic studies on stress tolerance of forage and turf grasses" 46 : 497-511, 2006

18 Chen C, "Real–time quantification of microRNAs by stem–loop RT–PCR" 33 : e179-, 2005

19 Kirstein-Miles J, "Peptides signal mitochondrial stress" 11 : 177-178, 2010

20 Patel M, "Overexpression of ubiquitin-like LpHUB1 gene confers drought tolerance in perennial ryegrass" 13 : 689-699, 2015

21 Dai XY, "Over-expression of an R1R2R3 MYB gene, OsMYB3R-2, increases tolerance to freezing, drought and salt stress in transgenic Arabidopsis" 143 : 1739-1751, 2007

22 Vidal EA, "Nitrate-responsive miR393/AFB3regulatory module controls root system architecture in Arabidopsis thaliana" 107 : 4477-4482, 2010

23 Capell T, "Modulation of the polyamine biosynthetic pathway in transgenic rice confers tolerance to drought stress" 101 : 9909-9914, 2004

24 Khan GA, "MicroRNAs as regulators of root development and architecture" 77 : 47-58, 2011

25 Matthew W, "MicroRNAs and Their Regulatory Roles in Plants" 57 : 19-53, 2006

26 Xiang L, "MicroRNA393 is involved in nitrogen-promoted rice tillering through regulation of auxin signal transduction in axillary buds" 6 : 1-12, 2016

27 Guo HS, "MicroRNA directs mRNA cleavage of the transcription factor NAC1 to down-regulate auxin signals for Arabidopsis lateral root development" 17 : 1376-1386, 2005

28 Tran LS, "Isolation and functional analysis of Arabidopsis stressinducible NAC transcription factors that bind to a drought-responsive cis-element in the early responsive to dehydration stress 1promoter" 16 : 2481-2498, 2004

29 Ashraf M, "Inducing drought tolerance in plants : recent advances" 28 : 169-183, 2010

30 Morran S, "Improvement of stress tolerance of wheat and barley by modulation of expression of DREB/CBF factors" 9 : 230-249, 2011

31 Taji T, "Important roles of drought and cold inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana" 29 : 417-426, 2002

32 Li Z, "Heterologous expression of OsSIZ1, a rice SUMO E3 ligase, enhances broad abiotic stress tolerance in transgenic creeping bentgrass" 11 : 432-445, 2013

33 Gonzalez L, "Handbook of plant ecophysiology techniques" Kluwer Academic Publishers 207-212, 2001

34 Babu RC, "HVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice(Oryza sativa L. )via cell membrane protection" 166 : 855-862, 2004

35 Dongli H, "Genome-Wide Dissection of the MicroRNA Expression Profile in Rice Embryo during Early Stages of Seed Germination" 12 : 1-21, 2015

36 Hu H, "Genetic engineering and breeding of drought resistant crops" 65 : 715-741, 2014

37 Waditee R, "Genes for direct methylation of glycine provide high levels of glycinebetaine and abiotic-stress tolerance in Synechococcus and Arabidopsis" 102 : 1318-1323, 2005

38 Arnaud TDT, "Functional roles of microRNAs in agronomically important plants—potential as targets for crop improvement and protection" 378 : 1-24, 2017

39 Jang IC, "Expression of a bifunctional fusion of the Escherichia coli genes for trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase in transgenic rice plants increases trehalose accumulation and abiotic stress tolerance without stunting growth" 131 : 516-524, 2003

40 Patel M, "Enhancing Agrobacterium tumefaciens-mediated transformation efficiency of perennial ryegrass and rice using heat and high maltose treatments during bacterial infection" 114 : 19-29, 2013

41 Qi CH, "Embryogenic callus induction and plant regeneration of Zoysia japonica" 13 : 257-259, 2005

42 Yamada M, "Effects of free proline accumulation in petunias under drought stress" 56 : 1975-1981, 2005

43 Lichtenthaler HK, "Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents" 11 : 591-592, 1983

44 Agarwal PK, "Bioengineering for salinity tolerance in plants : state of the art" 54 : 102-123, 2013

45 Kemal Kazan, "Auxin and the integration of environmental signals into plant root development" 26 : 1-11, 2015

46 Oh SJ, "Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth" 138 : 341-351, 2005

47 Fry J, "Applied turfgrass science and physiology" Wiley 2004

48 Chai B, "Applications of biotechnology in turfgrass genetic improvement" 38 : 1320-1338, 1998

49 Anastasia Giannakoula M, "Aluminum tolerance in maize are correlated with increased levels of mineral nutrients, carbohydrates and proline, and decreased levels of lipid peroxidation and Al accumulation" 165 : 385-396, 2008

50 Yu TT, "Agrobacterium-mediated transformation of creeping bentgrass using GFP as a reporter gene" 133 : 229-234, 2000

51 Chen H, "A plant microRNA regulates the adaptation of roots to drought stress" 586 : 1742-1747, 2012

52 Dellaporta S, "A plant DNA minipreparation, version II" 1 : 19-21, 1983

53 Bajaj S, "A high throughput Agrobacterium tumefaciens mediated transformation method for functional genomics of perennial ryegrass(Lolium perenne L. )" 25 : 651-659, 2006

54 Lionel Navarro P, "A Plant miRNA Contributes to Antibacterial Resistance by Repressing Auxin Signaling" 312 : 436-, 2006