Leucobacter denitrificans sp. nov., Isolated from Cow Dung

다국어 초록 (Multilingual Abstract)

The bacterial strain M1T8B10T was isolated from cow dung in Suwon, Republic of Korea. The strain was a Gram stainpositive rod, nonmotile, and non-spore-forming. According to 16S rRNA gene sequence analysis, the strain fell within the clade of the genus Leucobacter, showing the highest sequence similarities with Leucobacter aridicollis L-9T (98.7%), Leucobacter iarius 40T (98.4%), and Leucobacter komagatae JCM 9414T (98.2%). Cell-wall peptidoglycan contained the diagnostic diamino acid 2,4-diaminobutyric acid of the genus Leucobacter, showing B-type cross-linked peptidoglycans. The major fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0. The quinone system consisted of the menaquinones MK-11 (78%) and MK-10 (22%). The polar lipid profiles contained diphosphatidylglycerol, phosphatidylglycerol, and an unidentified glycolipid. Differences in several physiological features including nitrate reduction enabled the isolate to be differentiated from all recognized Leucobacter species. Based on these phylogenetic, chemotaxonomic, and phenotypic results, the isolate represents a novel species, for which the name Leucobacter denitrificans sp. nov. is proposed. The type strain is M1T8B10T (=KACC 14055T =NBRC 106309T).

참고문헌 (Reference)

1 Lin, Y.C., "Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae" 54 : 1669-1676, 2004

2 Smibert, R.M., "Phenotypic characterization, In Methods for General and Molecular Bacteriology" American Society for Microbiology 1994

3 Schleifer, K.H., "Peptidoglycan types of bacterial cell walls and their taxonomic implications" 36 : 407-477, 1972

4 Weon, H. Y., "Niastella koreensis gen. nov., sp. nov. and Niastella yeongjuensis sp. nov., novel members of the phylum Bacteroidetes, isolated from soil cultivated with Korean ginseng" 56 : 1777-1782, 2006

5 Kumar, S., "MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment" 5 : 150-163, 2004

6 Behrendt, U., "Leucobacter tardus sp. nov., isolated from the phyllosphere of Solanum tuberosum L" 58 : 2574-2578, 2008

7 Morais, P. V., "Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress" 29 : 414-421, 2006

8 Takeuchi, M., "Leucobacter komagatae gen. nov., sp. nov., a new aerobic Grampositive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall" 46 : 967-971, 1996

9 Somvanshi, V. S., "Leucobacter iarius sp. nov., in the family Microbacteriaceae" 57 : 682-686, 2007

10 Sturm, G., "Leucobacter chromiiresistens sp. nov., a novel chromate-resistant strain in the genus Leucobacter" 61 : 956-960, 2010

11 Muir, R.E., "Leucobacter chromiireducens subsp. solipictus subsp. nov., a pigmented bacterium isolated from the nematode Caenorhabditis elegans, and emended description of L. chromiireducens" 57 : 2770-2776, 2007

12 Morais, P. V., "Leucobacter chromiireducens sp. nov., and Leucobacter aridicollis sp. nov., two new species isolated from a chromium contaminated environment" 27 : 646-652, 2004

13 Halpern, M., "Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass" 59 : 665-670, 2009

14 Martin, E., "Leucobacter aerolatus sp. nov., from the air of a duck barn" 60 : 2838-2842, 2010

15 Sasser, M., "Identification of bacteria by gas chromatography of cellular fatty acids" MIDI Inc. 1990

16 김세일, "EzTaxon: A web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences" Society for General Microbiology 57 (57): 2259-2261, 200710

17 Seldin, L., "Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains" 35 : 151-154, 1985

18 Thompson, J. D., "CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice" 22 : 4673-4680, 1994

19 Minnikin, D. E., "An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids" 2 : 233-241, 1984