TMT 유도성 인지 기능 상실에 대한 대보(밤 품종) 내피 추출물의 효과

국문 초록 (Abstract)

본 연구에서는 국내 대표 밤 품종인 대보(Daebo)의 내피를 활용하여 TMT 유도성 인지 기능 상실에 대한 개선 효과를 연구하였다. 실험에서 각 농도별(5, 10, 20 ㎎/㎏ of body weight)로 대보 내피 에틸아세테이트 분획물을 섭취한 mouse를 TMT로 인지기능 손상을 유발하여 Y-maze test와 passive avoidance test한 결과, Y-maze test에서 분획물을 섭취한 group이 TMT 단독 처리군과 비교하였을 때 공간 인지기능을 개선시켰고, passive avoidance test 또한 latency time이 증가한 것으로 나타나 TMT에 의해 유발되는 뇌 신경독성 동물 모델로부터 기억 및 학습능력 개선 효과를 갖는 것으로 확인되었다. 또한 in vivo 동물 실험 후 mouse로부터 적출된 whole brain tissue를 대상으로 ex vivo AChE 활성 및 MDA 함량측정 실험한 결과, 에틸아세테이트 분획물이 TMT 효과 대비 AChE의 활성을 일부 유의적으로 억제하는 것을 알 수 있었다. 결론적으로 대보 내피 에틸아세테이트 분획물은 신경전달물질인 AChE의 활성을 저해하고 뇌 신경세포 보호 효과를 통하여 인지기능 개선 효과를 유도할 수 있는 것으로 사료된다.

다국어 초록 (Multilingual Abstract)

The aim of this study was to investigate the anti-amnesic effect of daebo (Castanea crenata) extract on trimethyltin chloride (TMT)-induced learning and memory impairment, in vivo. The inner skin of daebo was extracted using distilled water under reflux conditions. At the end of the adaptation period, ICR mice were divided into a control group, a TMT injection group (negative control), and a sample group (C5: 5 ㎎/㎏ body weight; C10: 10 ㎎/㎏ body weight; and C20: 20 ㎎/㎏ body weight), and were tested with learning and memory tests. The ethylacetate fraction of the daebo inner skin extract was found to increase TMT-induced memory deficit in the Y-maze and passive avoidance test. Brain tissue analysis showed that the ethylacetate fraction of daebo extract lowered the acetylcholine esterase (AChE) activity and malondialdehyde (MDA) content of neuronal cells, both of which are indicative of lipid peroxidation.

목차 (Table of Contents)

• Abstract
• 서론
• 재료 및 방법
• 결과 및 고찰
• 요약
• References

참고문헌 (Reference)

1 서일원, "밤가공품 중 polycyclic aromatic hydrocarbons 함량 분석" 한국식품과학회 41 (41): 339-344, 2009

2 김용두, "밤 부위별 추출물의 항균활성" 한국식품저장유통학회 12 (12): 257-262, 2005

3 황자영, "박피 밤의 저장온도에 따른 품질 특성 변화" 한국식품영양학회 24 (24): 71-78, 2011

4 Son CH, "Trend and future of the domestic industry for chestnut" Korea Rural Economics Institute 77-79, 2003

5 Hwang TY, "The effects of microwave heating of the texture of sugared chestnuts" 30 : 569-573, 1998

6 Ha BS, "Studies on constituent variation during storage after freeze-drying of chestnut" 14 : 97-105, 1982

7 Snow RE, "Psychosis in neurodegenerative disease" 1 : 282-293, 1996

8 Park IS, "Physicochemical properties of chestnut starch" 25 : 218-223, 1982

9 Kim SK, "Physicochemical and textural properties of chestnut starches" 24 : 594-600, 1995

10 Kim JS, "Marketing of chestnut and economic analysis of chestnut cultivation" 12 : 12-21, 2004

11 Kim JK, "Inhibitory effect of poncirus trifoliate on acetylcholinesterase and attenuating activity against trimethyltin-induced learning and memory impairment" 73 : 1105-1112, 2009

12 Loullis CC, "Hippocampal muscarinic receptor loss following trimethyl tin administration" 22 : 147-151, 1985

13 Kim MJ, "Ferulic acid supplementation prevents trimethyltin-induced cognitive deficits in mice" 71 : 1063-1068, 2007

14 LeDoux JE, "Emotional memory system in the brain" 20 : 69-79, 1993

15 Kim JK, "Effects of methoxsalen from poncirus trifoliata on acetylcholinesterase and trimethyltininduced learning and memory impairment" 75 : 1984-1989, 2011

16 Choi GN, "Effect of quercetin on learning and memory performance in ICR mice under neurotoxic trimethyltin exposure" 132 : 1019-1024, 2012

17 Kim MJ, "Characteristics and cultivation management about chestnut new varieties as daebo, daehan and mipung" National Forestry Cooperative Federation 81-85, 2010

18 Earley B, "Behavioural, biochemical and histological effects of trimethytin (TMT) induced brain damage in the rat" 21 : 351-366, 1992

19 Sarter M, "Attenuation of scopolamine induced impairment of spontaneous alternation behavior by antagonist but not inverse agonist and beta-carbolines" 94 : 491-495, 1988

20 Chang NS, "Antioxidative effects of green tea power diet against ethanol-induced oxidative damage in rat brain regions" 34 : 525-531, 2001

21 Hee Rok Jeong, "Antiamnesic Effects of Ethyl Acetate Fraction from Chestnut (Castanea crenata var. dulcis) Inner Skin on Ab25–35-Induced Cognitive Deficits in Mice" 한국식품영양과학회 15 (15): 1051-1056, 2012

22 Choi SJ, "Ameliorative effect of 1.2-benzenedicarboxylic acid dinonyl ester against amyloid beta peptide-induced neurotoxicity" 16 : 15-24, 2009

23 Whitehouse PJ, "Alzheimer’s disease: Evidence for selective loss of cholinergic neurons in the nucleus basalis" 10 : 122-126, 1981

24 Coyle JT, "Alzheimer’s disease: A disorder of cortical cholinergic innervation" 219 : 1184-1190, 1983

25 Kidd PM, "Alzheimer’s disease, amnestic mild cognitive impairment, and age-associated memory impairment: Current understanding and progress toward integrative prevention" 13 : 85-115, 2008

26 Talesa VN, "Acetylcholinesterase in alzheimer’s disease" 122 : 1961-1969, 2001

27 Ellman GL, "A new and rapid colorimetric determination of acetylcholinesterase activity" 7 : 88-95, 1961