CMCase를 생산하는 Klebsiella sp. Sc를 부산 인근의 토양에서 분리하였다. 이 균주는 16S rDNA와 RNA polymerase B subunit (rpoB) 유전자의 유전적 계통 분석을 통하여 동정을 시행하였다. 그 결과 Klebsiella sp....
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https://www.riss.kr/link?id=A76263835
2008
Korean
KCI등재
학술저널
639-645(7쪽)
1
0
상세조회0
다운로드국문 초록 (Abstract)
CMCase를 생산하는 Klebsiella sp. Sc를 부산 인근의 토양에서 분리하였다. 이 균주는 16S rDNA와 RNA polymerase B subunit (rpoB) 유전자의 유전적 계통 분석을 통하여 동정을 시행하였다. 그 결과 Klebsiella sp....
CMCase를 생산하는 Klebsiella sp. Sc를 부산 인근의 토양에서 분리하였다. 이 균주는 16S rDNA와 RNA polymerase B subunit (rpoB) 유전자의 유전적 계통 분석을 통하여 동정을 시행하였다. 그 결과 Klebsiella sp. 속들의 균주들과 99% 이상의 상동성을 나타내었다. CMCase 유전자는 Sc의 게놈라이브러리를 구축하여 찾았다. CMCase 유전자는 37,666 Da의 분자량을 가지는 333개의 아미노산을 암호화하고 있는 1,002개의 뉴클레오티드들로 구성된 것으로 밝혀졌다. 추정된 CMCase의 단백질 서열은 Cellulomonas uda와 단백질과 95%의 identity와 97%의 positive를 가지는 것으로 나타났으며, Erwinia chrysanthemi와는 각각 58%와 72%를 가지는 것으로 나타났다. 그리고 CMCase는 ASDGDTLIAWALLRAQ KQW와 같은 보존 영역을 가지고 있었는데, 이는 glycosyl hydrolase family 8의 보존영역(A-[ST]-D-[AG]-D-X(2)-[IM]-A-[SA]-[LIVM]-[LIVMG]-A-X(3)-[FW])와 일치하는 것으로 나타났다. 이는 Klebsiells sp. Sc의 CMCase는 glycosyl hydrolase family 8에 속한다는 것을 나타낸다.
다국어 초록 (Multilingual Abstract)
A CMCase-producing bacterium, Klebsiella sp. Sc, was isolated from Busan area's soil sample. This strain was identified on the basis of phylogenetic analysis of the 16S rDNA and rpoB (RNA polymerase B subunit) sequences. The gene encoding CMCase was c...
A CMCase-producing bacterium, Klebsiella sp. Sc, was isolated from Busan area's soil sample. This strain was identified on the basis of phylogenetic analysis of the 16S rDNA and rpoB (RNA polymerase B subunit) sequences. The gene encoding CMCase was cloned by genome library. The CMCase gene consisted of an open reading frame of 1,002 nucleotides and encodes 333 amino acids with a deduced molecular weight of 37,666 Da. The deduced amino acid sequence of the CMCase from Klebsiella sp. exhibits 95% identities and 97% positives to those from Cellulomonas uda and 58% identities and 72% positives to those from Erwinia chrysanthemi. The CMCase contain a conserved motif with the consensus sequence, ASDGDTLI AWALLRAQKQW. This result shows CMCase protein from Klebsiella sp. Sc belongs to glycoside hydrolase family 8, A-[ST]-D-[AG]-D-X(2)-[IM]-A-[SA]-[LIVM]-[LIVMG]-A-X(3)-[FW].
참고문헌 (Reference)
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1 Mollet, C., "rpoB sequence analysis as a novel basis for bacterial identification" 26 : 1005-1011, 1997
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4 Alzari, P. M, "The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum" 4 : 265-275, 1996
5 Thompson, J. D., "The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools" 24 : 4876-4882, 1997
6 Nakamura, K., "Sequence of a cellulase of Cellulomonas uda CB4" 4 : 247-254, 1986
7 Beguin, P., "Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum" 162 : 102-105, 1985
8 Guiseppi, A., "Sequence analysis of the cellulase-encoding celY gene of Erwinia chrysanthemi: a possible case of interspecies gene transfer" 106 : 109-114, 1991
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체외순환 비적용 관상동맥우회 수술 중 체외순환술식으로의 전환: 예측인자 및 임상결과의 분석
학술지 이력
연월일 | 이력구분 | 이력상세 | 등재구분 |
---|---|---|---|
2027 | 평가예정 | 재인증평가 신청대상 (재인증) | |
2021-01-01 | 평가 | 등재학술지 유지 (재인증) | ![]() |
2018-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2015-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2011-08-03 | 학술지명변경 | 외국어명 : Korean Journal of Life Science -> Journal of Life Science | ![]() |
2011-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2009-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2007-01-01 | 평가 | 등재학술지 유지 (등재유지) | ![]() |
2004-01-01 | 평가 | 등재학술지 선정 (등재후보2차) | ![]() |
2003-01-01 | 평가 | 등재후보 1차 PASS (등재후보1차) | ![]() |
2001-07-01 | 평가 | 등재후보학술지 선정 (신규평가) | ![]() |
학술지 인용정보
기준연도 | WOS-KCI 통합IF(2년) | KCIF(2년) | KCIF(3년) |
---|---|---|---|
2016 | 0.37 | 0.37 | 0.42 |
KCIF(4년) | KCIF(5년) | 중심성지수(3년) | 즉시성지수 |
0.43 | 0.43 | 0.774 | 0.09 |