영지(靈芝) 추출물이 Rat fetus 두개골로부터 분리한조골세포에 미치는 영향 = The Effects of Ganoderma Lucidum Extract on Osteoblast in Rat Fetus Calvarial Cells

다국어 초록 (Multilingual Abstract)

In this study, the author aimed to evaluate the effect of EtOH extractof Ganoderma lucidum (GLE) on osteoblast proliferation in rat fetus calvarial cells. Methods: The osteoblast separated from rat fetus calvariae was cultivated for6~21 days and evaluated the cell function. After the addition of GLE on the culturemedium, we determined the effect of GLE on the cell viability, cell proliferation,bone matrix protein synthesis, alkaline phosphatase (ALP) activity, collagen synthesisand calcified nodule formation of the cultivated osteoblast. Results: GLE did not change the survival rate of rat calvarial osteoblast. GLEincreased the proliferation of rat calvarial osteoblast. GLE increased ALP activityof rat calvarial osteoblast. GLE increased bone matrix protein synthesis of ratcalvarial osteoblast. GLE increased collagen synthesis of rat calvarial osteoblast. GLE slightly affected calcified nodule formation of rat calvarial osteoblast. Conclusions: This study suggests that Ganoderma lucidum might improve theosteoporosis resulted from augmentation of osteoblast proliferation.