신생흰쥐의 실험적 뇌경색-저산소에 대한 페니토인과 니트로글리세린의 효과 = THE NEUROPROTECTIVE EFFECTS OF PHENYTON AND TRANSDERMAL NITROGLYCERIN ON ISCHEMIC-HYPOXIC INJURY IN DEVELOPING RAT BRAINS

다국어 초록 (Multilingual Abstract)

The goal of this study was to evaluate the neuroprotective effects of phenytoin(PH) and nitroglycerin(NTG) using in the developing rat brains. Seven days old Sprague Dawley rats underwent right carotid ligation under the halothane anesthesia and then they stayed in the oxygen chamber(8% oxygen) for 3 hours in a awakened state with one of four paradigms : the control group with no medication(58), the PH-treated group receiving intraperitoneal injection of diphenylhydantoin(30mg/kg) before the oxygen camber session(17), the NTG-treated group with skin attachment of NTG(in escalating doses to 4mg/kg/hr) for 36hrs before and 48hrs post ischemia(33), and the PH plus NTG treated group(31). Temperature was controlled accurately before, and during hypoxia. The animals were sacrificed one week after ischemic-hypoxic injury. The weight disparities of the right and left were measured for the comparison(((L-R)/L)×100). The histologic study(HE stain) was made in each animal for comparison in neuronal loss among th groups.
PH was effective in reducing neuronal damage in terms of weight comparison(24±2.4% damage in the control vs.5±2.9% damage in th PH group, p<0.001) and degree of the histological changes especially in the hippocampal region(p<0.05 in CA1, CA2, and CA3 region). Transdermal NTG did not show any beneficial effects compared with the control group(23±3.0% vs 24±2.5%). The PH/NTG treated group had no additional effects compared with the PH-treated group on both weight comparison(4±1.8% vs 5±2.9%) developing rat brain may be reduced by the compounds that modulate voltage-dependent sodium channels such as PH but not by NTG.