솔잎혹파리의 변태에 따른 용기의 체액분석 = Studies on the Hemolymph during the Pupal Stages in the Pine Leaf Gall Midge, Thecodiplosis japonensis

다국어 초록 (Multilingual Abstract)

The concetration of total protein and non protein nitrogen, lipid, trehalose, acid$.$alkaline phosphatase, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and mineral salts during the pupal stage in the pine leaf gall midge, Thecodiptosis japonensis Uchida et Inouye, were measured by using micro-Kjeldahl method, Thin layer chromatographic method, Anthrone method, Reitman-Frankel method, Oser method and EEL dame photometric method respectively. Healthy specimens were chosen as samples of each developmental stages; the prepupa and the late pupa. The concentration of total protein was 88.139mg/g in the prepupa and was 120.719mg/g in the late pupal stage. Non protein nitrogen contents was 2.292mg/g in the prepupa add was 4.225mg/g in the late pupal stage. In the late pupal stage total protein and non protein nit-rogen contents was higher than those in the prepupal stage. Total lipid, phospholipid, menoacylgly cerol, free fatty acid, sterol and ester cholesterol were identified in the prepupa and the late pupal stage. The concentration of total lipid was 150.0mg/g in the prepupa and was 162.8mg/g in the late pupal stage. With the exception of total lipid, phospholipid, monoacylglycerol, free fatty acid, sterol and ester cholesterol contents were higher than in the late pupa those in the prepupal stage. Rf value of trehalose analyzed on thin layer chromatogram was 0.714 in both stages, and the concentration of trehalose was 3.640mg/g in the Prepupa and was 3.553mg/g in the late pupal stage. The activity of acid phosphatase was 0.272unit/mg in the prepupal and was 0.281unit/mg in the late pupal stage, and alkaline phosphatase was 0.413unit/mg in the prepupa and was 0.546unit/mg in the late pupal stage. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase showed the same patterns wi th acid . alkaline phosphatase. Mineral salts present were calcium, phosphorus, sodium and potassium, which were identified in both stages. All kinds of mineral salts contents in the late pupa were higher than those in the prepupal stage. In view of controlling pest, the patterns of the physiological activties indicate that control will be the most effective at the time of the worst physiological manifested at the prepupal stage.

국문 초록 (Abstract)

솔잎혹파리 (Thecodiplosis japonensis Uchida et Inouye)의 변태기 중 전용과 후용의 액체 변화를 분석하고 그 활성을 측정코자 micro-Kjeldahl법, Thin layer chromatography법, Anthrone법, Reitman-Fra-nkel법, Oser법, EEL flame photometer법으로 측정분석하였다. 1)전용과 후용에서 총단백질과 비단백성질소, 지질, 탄수화물, phosphatase, GOT, GPT, 무기염류의 함량과 화성도가 측정되었다. 2) 총단백질과 비단백성질소 모두 후용에서 높은 함량의 변화를 나타냈다. 3) 분석된 지질은 총지질, 인지질, monoacylalycerol, 유리지방산, sterol, triacylglycerol, ester cholesterol이였으며 triacylglycerol을 제외하고 후용에서 각 지질의 함량이 전용에서 보다 높았다. 4) trehalose의 함량은 큰 차이는 없었으나 전용에서 다소 높았다. 5) 효소 활성도의 변화는 acid.alkaline phosphatase, GOT, GPT 모두 후용에서 높았으며, GOT는 GPT에 비해 매우 높은 활성을 보였다. 6) 무기염류는 칼슘, 인, 나트륨, 칼륨이 분석되었으며, 칼륨이 가장 높은 함량을 나타냈다. 7) 전용과 후용의 생리적 활성을 비교하면 전용기가 생리적 기능하강기로 나타나 이 시기가 생물학적 방제의 최적기로 이용될 수 있을 것이다.