Immobilization of Acetobacter sp. CGMCC 8142 for Efficient Biocatalysis of 1, 3-propanediol to 3-hydroxypropionic Acid

다국어 초록 (Multilingual Abstract)

3-hydroxypropionic acid (3-HP) is an important chemical platform organic in material industry, daily chemical industry and biomedicine field due to its numerous valuable derivatives. However, no mature methods have been established in the synthesis industry for direct large scale production. The bacterium Acetobacter sp. CGMCC 8142 with high efficiency of alcohols oxidation property was immobilized for biosynthesis of 3-HP from 1, 3- propanediol (1, 3-PDO). Parameter values in mass transfer modeling indicated that mass transfer of immobilized biocatalysts affected the oxidation reaction (the internal effectiveness factor ηi < 1) but was not the rate-limiting step if Thiele modulus 1 > ϕ > 0.3. The optimal immobilization conditions for 3-HP biocatalysis was sodium alginate 40 g/L, gel beads diameter 1 mm, cross-linkage time 2 h and 0.1mM FeCl2. Immobilized cells showed promising substrate tolerance, pH stability, thermal stability and storability.
After 5 cycles of reaction, 3-HP molar yield of immobilized beads was retained to 80.26%, and 66.95 g/L 3-HP were produced from 70 g/L 1, 3-PDO. The biocatalysis process of immobilized cells introduced in this study may provide an economical and efficiency alternative route for practical production of 3-HP.

참고문헌 (Reference)

1 Takamizawa, K., "β-hydroxypropionic acid production by Byssochlamys sp. grown on acrylic acid" 40 : 196-200, 1993

2 Gomez-Manzo, S., "The active (ADHa) and inactive (ADHi) forms of the PQQ-alcohol dehydrogenase from Gluconacetobacter diazotrophicus differ in their respective oligomeric structures and redox state of their corresponding prosthetic groups" 328 : 106-113, 2012

3 Cozier, G. E, "Structure of the quinoprotein glucose dehydrogenase of Escherichia coli modelled on that of methanol dehydrogenase from Methylobacterium extorquens" 312 : 679-685, 1995

4 Luo, L. H., "Stimulation of reductive glycerol metabolism by overexpression of an aldehyde dehydrogenase in a recombinant Klebsiella pneumoniae strain defective in the oxidative pathway" 38 : 991-999, 2011

5 Radcliffe, D. F., "Sorption kinetics in haemoperfusion columns. Part 1: Estimation of mass-transfer parameters" 19 : 617-626, 1981

6 이상현, "Production of 3-Hydroxypropionic Acid from Acrylic Acid by Newly Isolated Rhodococcus erythropolis LG12" 한국미생물·생명공학회 19 (19): 474-481, 2009

7 Adachi, O, "New developments in oxidative fermentation" 60 : 643-653, 2003

8 Lan, E. I., "Metabolic engineering of cyanobacteria for photosynthetic 3-hydroxypropionic acid production from CO2 using Synechococcus elongatus PCC 7942" 31 : 163-170, 2015

9 Chu, H. S, "Metabolic engineering of 3-hydroxypropionic acid biosynthesis in Escherichia coli" 112 : 356-364, 2015

10 Lewinska, D., "Mass transfer coefficient in characterization of gel beads and microcapsules" 209 : 533-540, 2002

11 Fernandez-Lucas, J., "Low-temperature synthesis of 2' -deoxyadenosine using immobilized psychrotrophic microorganisms" 100 : 213-222, 2008

12 Miyano, K, "Improvement of vitamin B12 fermentation by reducing the inhibitory metabolites by cell recycle system and a mixed culture" 6 : 207-214, 2000

13 Chen, X. H, "Immobilization of Acetobacter sp. CCTCC M209061 for efficient asymmetric reduction of ketones and biocatalyst recycling" 11 : 119-, 2012

14 Garai-Ibabe, G., "Glycerol metabolism and bitterness producing lactic acid bacteria in cidermaking" 121 : 253-261, 2008

15 Kildegaard, K. R., "Engineering and systems-level analysis of Saccharomyces cerevisiae for production of 3-hydroxypropionic acid via malonyl-CoA reductase-dependent pathway" 15 : 53-, 2016

16 Raj, S. M, "Effect of process parameters on 3-hydroxypropionic acid production from glycerol using a recombinant Escherichia coli" 84 : 649-657, 2009

17 Rathnasingh, C, "Development and evaluation of efficient recombinant Escherichia coli strains for the production of 3-hydroxypropionic acid from glycerol" 104 : 729-739, 2009

18 Zhou, F. L., "Cloning and transcription analysis of the Candida rugosa propionyl-CoA dehydrogenase gene and its expression in Pichia pastoris" 52 : 360-367, 2012

19 Jiang, X. L., "Biosynthetic pathways for 3-hydroxypropionic acid production" 82 : 995-1003, 2009

20 Wang, Y., "Biosynthesis of platform chemical 3-hydroxypropionic acid (3-HP) directly from CO2 in cyanobacterium Synechocystis sp. PCC 6803" 34 : 60-70, 2015

21 Bommarius, A. S, "Biocatalysis" Wiley-VCH 105-547, 2004

22 Sieburth, J. M, "Antibiotic properties of acrylic acid, a factor in the gastrointestinal antibiosis of polar marine animals" 82 : 72-79, 1961

23 Qatibi, A. I., "Anaerobic degradation of glycerol by Desulfovibrio fructosovorans and D-carbinolicus and evidence for glycerol-dependent utilization of 1,2-propanediol" 36 : 283-290, 1998

24 Yakushi, T., "Alcohol dehydrogenase of acetic acid bacteria: Structure, mode of action, and applications in biotechnology" 86 : 1257-1265, 2010

25 Stieb, M, "A new 3-hydroxybutyrate fermenting anaerobe, Ilyobacter polytropus, gen. nov. sp. nov., possessing various fermentation pathways" 140 : 139-146, 1984