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    치과용 유니트 수관 물 빼기(Flushing)가 세균 오염도 감소에 미치는 영향 = Effects of Flushing in Dental Unit Waterlines on the Bacterial Contamination Level

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    https://www.riss.kr/link?id=A101248912

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    다국어 초록 (Multilingual Abstract) kakao i 다국어 번역

    A wide variety of methods have been used to control Dental Unit Waterline (DUWL) contamination. Among the methods, flushing is mainly used because it is simple and easy to use. Generally, flushing of DUWL for 20 or 30 sec before using high speed handpieces or scalers is recommended. However, the appropriateness of flushing time was not investigated thoroughly. The purpose of this study was to check the effective time of flushing for decreasing bacterial contamination. Seven dental unit chairs were randomly selected in student clinical simulation laboratory for this experiment. DUWLs were continuously flushed and water samples were collected at an interval of 30 seconds for 15 minutes. From five dental unit chairs, water samples were collected every 10 seconds for 1 minute. Bacterial levels in water samples were examined by the culture method on R2A plates. After 10 second flushing of DUWLs, the number of bacteria significantly reduced and decreased continuously up to 40 seconds. However, even after the water was flushed for 15 minutes, the bacterial contamination level was not reduced below recommended bacteria level, 200 CFU/ml. In addition to flushing, the periodic chemical disinfection is required to control the DUWL water to the recommended level.
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    A wide variety of methods have been used to control Dental Unit Waterline (DUWL) contamination. Among the methods, flushing is mainly used because it is simple and easy to use. Generally, flushing of DUWL for 20 or 30 sec before using high speed handp...

    A wide variety of methods have been used to control Dental Unit Waterline (DUWL) contamination. Among the methods, flushing is mainly used because it is simple and easy to use. Generally, flushing of DUWL for 20 or 30 sec before using high speed handpieces or scalers is recommended. However, the appropriateness of flushing time was not investigated thoroughly. The purpose of this study was to check the effective time of flushing for decreasing bacterial contamination. Seven dental unit chairs were randomly selected in student clinical simulation laboratory for this experiment. DUWLs were continuously flushed and water samples were collected at an interval of 30 seconds for 15 minutes. From five dental unit chairs, water samples were collected every 10 seconds for 1 minute. Bacterial levels in water samples were examined by the culture method on R2A plates. After 10 second flushing of DUWLs, the number of bacteria significantly reduced and decreased continuously up to 40 seconds. However, even after the water was flushed for 15 minutes, the bacterial contamination level was not reduced below recommended bacteria level, 200 CFU/ml. In addition to flushing, the periodic chemical disinfection is required to control the DUWL water to the recommended level.

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    참고문헌 (Reference)

    1 윤혜영, "치과대학 임상 시뮬레이션 실습실 치과용 유니트 수계의 세균 오염도 조사" 한국치위생과학회 15 (15): 232-237, 2015

    2 Martin MV, "The significance of the bacterial contamination of dental unit water systems" 163 : 152-154, 1987

    3 Al-Hiyasat AS, "The presence of Pseudomonas aeruginosa in the dental unit waterline systems of teaching clinics" 5 : 36-44, 2007

    4 Rice EW, "Standard methods for the examination of water and wastewater" American Public Health Association 2012

    5 Scheid RC, "Reduction of microbes in handpieces by flushing before use" 105 : 658-660, 1982

    6 Kettering JD, "Reducing bacterial counts in dental unit waterlines:Distilled water vs. antimicrobial agents" 30 : 735-741, 2002

    7 Barbeau J, "Multiparametric analysis of waterline contamination in dental units" 62 : 3954-3959, 1996

    8 Walker JT, "Microbiological evaluation of dental unit water systems in general dental practice in europe" 112 : 412-418, 2004

    9 Uzel A, "Microbiological evaluation and antibiotic susceptibility of dental unit water systems in general dental practice" 6 : 43-47, 2008

    10 Williams JF, "Microbial contamination of dental unit waterlines: Prevalence, intensity and microbiological characteristics" 124 : 59-65, 1993

    1 윤혜영, "치과대학 임상 시뮬레이션 실습실 치과용 유니트 수계의 세균 오염도 조사" 한국치위생과학회 15 (15): 232-237, 2015

    2 Martin MV, "The significance of the bacterial contamination of dental unit water systems" 163 : 152-154, 1987

    3 Al-Hiyasat AS, "The presence of Pseudomonas aeruginosa in the dental unit waterline systems of teaching clinics" 5 : 36-44, 2007

    4 Rice EW, "Standard methods for the examination of water and wastewater" American Public Health Association 2012

    5 Scheid RC, "Reduction of microbes in handpieces by flushing before use" 105 : 658-660, 1982

    6 Kettering JD, "Reducing bacterial counts in dental unit waterlines:Distilled water vs. antimicrobial agents" 30 : 735-741, 2002

    7 Barbeau J, "Multiparametric analysis of waterline contamination in dental units" 62 : 3954-3959, 1996

    8 Walker JT, "Microbiological evaluation of dental unit water systems in general dental practice in europe" 112 : 412-418, 2004

    9 Uzel A, "Microbiological evaluation and antibiotic susceptibility of dental unit water systems in general dental practice" 6 : 43-47, 2008

    10 Williams JF, "Microbial contamination of dental unit waterlines: Prevalence, intensity and microbiological characteristics" 124 : 59-65, 1993

    11 Walker JT, "Microbial biofilm formation and contamination of dental-unit water systems in general dental practice" 66 : 3363-3367, 2000

    12 Bartoloni JA, "Measuring the validity of two in-office water test kits" 137 : 363-371, 2006

    13 Ma'ayeh SY, "Legionella pneumophila contamination of a dental unit water line system in a dental teaching centre" 6 : 48-55, 2008

    14 Atlas RM, "Legionella contamination of dental-unit waters" 61 : 1208-1213, 1995

    15 Porteous NB, "Isolation of non-tuberculosis mycobacteria in treated dental unit waterlines" 98 : 40-44, 2004

    16 Whitehouse RL, "Influence of biofilms on microbial contamination in dental unit water" 19 : 290-295, 1991

    17 Cobb CM, "How does time-dependent dental unit waterline flushing affect planktonic bacteria levels?" 66 : 549-555, 2002

    18 easoner DJ, "Heterotrophic plate count methodology in the united states" 92 : 307-315, 2004

    19 Kohn WG, "Guidelines for infection control in dental health care settings-2003" 135 : 33-47, 2004

    20 Schulze-Robbecke R, "Dental units: An environmental study of sources of potentially pathogenic mycobacteria" 76 : 318-323, 1995

    21 Williams HN, "Contribution of biofilm bacteria to the contamination of the dental unit water supply" 126 : 1255-1260, 1995

    22 Karpay RI, "Comparison of methods to enumerate bacteria in dental unit water lines" 38 : 132-134, 1999

    23 Shepherd PA, "Clearance of biofilms from dental unit waterlines through the use of hydroperoxide ion-phase transfer catalysts" 32 : 755-761, 2001

    24 Shearer BG, "Biofilm and the dental office" 127 : 181-189, 1996

    25 Mayo JA, "Bacterial biofilm: A source of contamination in dental air-water syringes" 12 : 13-20, 1990

    26 Lee BM, "A study on the microbial contamination of dental unit and ultrasonic scaler" 36 : 64-80, 1998

    27 Reasoner DJ, "A new medium for the enumeration and subculture of bacteria from potable water" 49 : 1-7, 1985

    28 Porteous N, "A comparison of 2laboratory methods to test dental unit waterline water quality" 77 : 206-208, 2013

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