국내 생산단계 토마토의 위생지표세균 및 유해미생물 오염도 조사 = Survey of Microbial Contamination of Tomatoes at Farms in Korea

다국어 초록 (Multilingual Abstract)

This study investigated and evaluated contamination levels of bacteria on tomatoes at farms stage to evaluate potential hazards associated with fresh tomatoes. A total of 170 samples, 90 samples from 5 sampling sites from 18 farms and 80 samples from 1 sampling site from 4 farms every month for four months, were analyzed to enumerate aerobic bacterial counts, coliforms, E. coli, Bacillus cereus, Staphylococcus aureus, Escherichia coli, E. coli O157:H7, Salmonella spp., and Listeria monocytogenes. Aerobic bacterial counts ranged from 0.48 to 6.15 Log CFU/g, with the lowest and the highest bacterial cell counts recorded for A site and E site, respectively. Thirty five percent of the samples from the E site contained more than 2 Log CFU/g. Six samples (6.6%) of 90 samples contained B.
cereus less than 1 Log CFU/g. In addition, the contamination level of indicator bacteria and B. cereus in tomatoes were higher on March than on April, May and June (P < 0.05). S. aureus, E. coli, E. coli O157:H7, Salmonella spp.
and L. monocytogenes were not detected in the tomato samples. The microbial contamination levels of tomatoes determined in this study may be used as the data for microbiological risk assessment.

국문 초록 (Abstract)

본 연구는 생산단계 토마토를 국내 5개 지역 소재의 18개 농가에서 총 90점의 시료를 수집하여 위생지표세균 및유해미생물의 오염도를 분석하였다. 또한 주산지 1개 지역의 4개 농가를 대상으로 시기별(3, 4, 5, 6월) 위생지표세균 및 유해미생물의 오염도 변화를 조사하였다. 생산단계 토마토의 위생지표세균 중 일반호기성세균은 최소 0.48- 최대 6.15 Log CFU/g 범위로 나타났고, Coliforms는 최소ND - 최대 3.37 Log CFU/g 범위로 나타났다. B. cereus의경우에는 90개 시료 중 6개의 시료에서 검출되었고 모두1 LogCFU/g 이하인 것으로 나타났다. 시기별로는 3월에일반호기성세균, Coliforms, B. cereus가 평균적으로 각각2.70, 0.56, 1.58 Log CFU/g으로 나타났고, 이들 모두 4, 5,6월로 시기가 지남에 따라 오염도가 통계적으로 유의하게낮아지는 것으로 나타났다. 본 연구에서 수행한 S. aureus,E. coli, E. coli O157:H7, Salmonella spp., L. monocytogenes오염도 조사에서는 이들 모두 불검출로 나타났다. 본 연구는 국내 생산단계 토마토의 위생지표세균 및 유해미생물 오염도를 조사하고 시기별 오염도 변화 양상을 관찰하여, 농식품 안전성 확보 및 국내 토마토의 미생물위해성평가(Microbiological Risk Assessment)의 기초자료로 활용될 수 있을 것이다.

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