The lead resistant bacteria were isolated from the samples of the sod or water around the mine. The strain 29 and 57 were most reliable resistant effect among the isolates. The stram 29 and 57 were grown on the medium contaning 4㎎/㎖ of lead. The p...
The lead resistant bacteria were isolated from the samples of the sod or water around the mine. The strain 29 and 57 were most reliable resistant effect among the isolates. The stram 29 and 57 were grown on the medium contaning 4㎎/㎖ of lead. The plasmid DNA was investegated to know the mechanism of the lead resistance. The lysis of the bacterial cell envelop was conformed by treatment of 1㎎/㎖ of lysozyme for 15 min. The plasmid of 21kb was extracted by rapid alkaline method. The lead resistant phenomenon was disappeared by treatment with curing agent : SDS or EtBr. So, the lead resistant factor was located within the plasmid.