尿에서 分離한 Gram 陰性菌의 分布 및 抗菌劑 耐性 = Antimicrobial Susceptibility of Organisms Isolated from Bacteriuria

국문 초록 (Abstract)

1983년 소변에서 11종 116주의 Gram음성간균을 분리하였는데 E. coli가 47주로 가장 많았고, Klebsiella pneumoniae가 12주, Serratia marcescens가 7주였다.
동정한 10종 107주의 균을 대상으로 16종의 항균제에 대한 최소발육저지농도(MIC)를 조사하였는데 90%의 균주의 발육을 저지하는 MIC가 E. coli, Klebsiell, Proteus에서는 cefotaxime(Ct)이 가장 낮았고, amikacin(Am), nalidixic acid(Na), Rifampin(Rf), gentamicin(Gm)의 순이었으며, P. aeruginosa는 Ct, moxalactam, tobramycin, Na, Rf를 제외하고는 모두 실험한 최고농도인 512㎍/㎖ 또는 2,048㎍/㎖ 이상이었다. 분리된 내성균을 대상으로 E. coli ML 1410 또는 RG 488로 내성전달 실험을 하였는데 E. coli 42주중 20주(48%), K. pneumoniue 23주중 13주(57%) Proteus 12주중 3주, Citrobacter 2주 중 1주에서 내성전달이 되었다.
약제별 전달빈도는 sulfisomidine에서 66주의 내성주 중 33주(50%)로 가장 높았으며, kanamycin(49.0%), tetracycline(45.6%), ampicillin(42.9%), trimethoprim(42.1%), chloramphenicol(39.6%)의 순이었다.

다국어 초록 (Multilingual Abstract)

A total 116 strains of gram negative bacteria affiliated to 11 kinds of species were isolated from urine of patients with urologic diseases in 1983.
Fortyseven strains of EschricJtte mfi(E. culi) found to he most frequent organisms an& followed by KldsielZa pneumniae(24 strains), Pseudomonas aeruginosa and Prutezz each), and S e r r d a mrcacens(7 strains) in decreasing order.
A hundred and seven strains which were available for definkte identification of its species were subjected for antimicrobial susceptibility test to 16 drugs. Minharn inhibhry COAIX~~K- ation needed to inhibit growth of 90% of strains@41Cgo : ,ug/d) were determined. E. cdi, Klebsidiu, and Proteus species showed the lowest level of MIGO against cofotaxime(Ct) and followed by amikacin(Ak), nalidixic acidma), rifampin(Rf), and gentarnicin(Gm) in order of higher level of concernation. MIGO of Pseudomoms aerugimsa against lQ drugs except CE,. Na, Rf, moxalactam, and tobramycin were higher than 512pg/ml or 2,048pg4'd.
Test for transferable resistance mediated by R plasmid resulted that 20 strains@fj%) of E.. coli, 1337%) of KZebsida, 3125%) of Proteus, and 1 of 2 strains of Gitrobacier transferred their resistance to recipient stains, E. coli ML,1410 or RGa8 by conjugation. Thirty-thee (50%) of 66 strains resistant to sulfisornidine tramferred their resistance, which was found to Gghest freqnency of transferability among dmgs tested. Frequency of. transferable resistance of kanamycin were 49%, tetracycline 45.67&, amgicillin 42.9%, trirnethoprim 42.1%, and chloramphenicol 39.6%'0.