遺傳子 發現用 Promoter 開發에 關한 硏究 = Studies on the Development of Promoter for the Gene Expression

국문 초록 (Abstract)

본 연구는 lac'z gene의 promoter개발을 위하여 착수하였다. lac'z gene의 promoterⅠ과 Ⅱ를 효모의 염색체 Bam HI DNA 단편에서 발견하였다. PromoterⅠ의 크기의 2.5kb정도이고 β- galactosidase 활성은 124.6 U/㎎ protein이었으나 promoterⅡ의 크기와 효소활성은 4.0kb 와 168.8 U/㎎이었다.
형질전환체에서 YEp plasmid 안정성은 52.7%에서 67.4%정도였다. YEp plasmid에서 YIp plasmid를 구출할 수 있었고 이 YIp plasmid는 대장균에서나 효모에서도 발현되었고 특히 HIS5 gene이 promoter로서 가능함을 알 수 있었다.

다국어 초록 (Multilingual Abstract)

It was attempted to observe the development of promoter on lac'z gene. Two promoterⅠand Ⅱ of lac'z gene were isolated from chromosomal DNA Bam HI fragment of yeast. The size of the promoterⅠwas estimated to be 2.5kb and β- galactosidase activity was 124.6 U/㎎ protein but the size of the promoterⅡ was 4.0kb and its β- galactosidase activity was 168.8 U/㎎ protein respectively. The stability of the recombinant YEp plasmid in the transformant was from 52.7 to 67.4% at minimal medium. And YIp plasmid was constructed by YEp plasmid. This YIp plasmid both in E.coli and yeast. The HIS5 gene, coding gene of histidiolphosphate aminotransferase functioned as the promoter of YIp plasmid .