Production and characterization of anti-Salmonella polyclonal antibodies as bio-recognition element for developing a microbial monitoring method

다국어 초록 (Multilingual Abstract)

For the construction of the microbial monitoring method, anti-Salmonella polyclonal antibodies (pAbs) were produced from a rabbit and purified by saturated ammonium sulfate precipitation and protein A affinity column. The reactivity of anti-Salmonella pAbs was compared to that of commercial ones by using an indirect ELISA. The specificity of anti-Salmonella pAbs was investigated using 20 Salmonella serotypes and 20 non-Salmonella strains. A capturing ability of anti-Salmonella pAbs was investigated by exposing antibody-immobilized gold biosensor to different concentration of Salmonella mixture. Anti-Salmonella pAbs were successfully produced and purified with an antibody concentration of 2.0 mg/mL The reactivity of purified anti-Salmonella pAbs was greater than that of commercial one at all tested concentrations. All Salmonella serotypes, except S. Diarizonae, showed excellent binding efficiency with purified anti-Salmonella pAbs. Moreover, the purified anti-Salmonella pAbs showed excellent specificity against all non-Salmonella strains. The anti-Salmonella pAbs immobilized on the gold biosensor demonstrated the successful capturing capability against Salmonella with a dose-response manner. Therefore, the anti-Salmonella pAbs exhibited sufficient reactivity, specificity, as well as capturing capability against Salmonella to be considered as a bio-recognition element.

참고문헌 (Reference)

1 Centers for Disease Control and Prevention,

2 Tirado C, "WHO Surveillance program for control of foodborne infections and intoxications: preliminary results and trends across greater Europe" 43 : 80-84, 2001

3 Skottrup PD, "Towards on-site pathogen detection using antibody-based sensors" 24 : 339-348, 2008

4 Park MK, "The effect of incubation temperature on the binding of Salmonella typhimurium to phage-based magnetoelastic biosensors" 160 : 1427-1433, 2011

5 Centers for Disease Control and Prevention, "Surveillance for foodborne-disease outbreaks in the United States, 1998-2002" 55 : 1-34, 2006

6 Zeng X, "Recombinant antibodies and their use in biosensors" 402 : 3027-3038, 2012

7 Park MK, "Rapid detection of Escherichia coli O157:H7 on turnip greens using a modified gold biosensor combined with light microscopic imaging system" 77 : M127-M134, 2012

8 Park MK, "Optimization and application of a dithiobis-succinimidyl propionate-modified immunosensor platform to detect Listeria monocytogenes in chicken skin" 171-172 : 323-331, 2012

9 Su L, "Microbial biosensors: a review" 26 : 1788-1799, 2011

10 Park MK, "Immunomagnetic bead separation coupled with a dithiobis-succinimidyl propionate(DSP)-modified immunosensor to detect Listeria monocytogenes in chicken skin" 161 : B237-B242, 2014

11 Plaza G, "Immunoassays and environmental studies" 9 : 231-236, 2000

12 Currie A, "Frozen chicken nuggets and strips and eggs are leading risk factors for Salmonella Heidelberg infections in Canada" 133 : 809-816, 2005

13 Mead PS, "Food-related illness and death in the United States" 5 : 607-625, 1999

14 Nataro JP, "Escherichia, Shigella, and Salmonella" ASM press 354-383, 2011

15 Deshpande SS, "Enzyme immunoassays from concept to product development" Springer International Publishing AG 24-154, 1996

16 Danczyk R, "Comparison of antibody functionality using different immobilization methods" 84 : 215-223, 2003

17 "Centers for Disease Control and Prevention"

18 Turner PF, "Biosensors : sense and sensibility" 42 : 3184-3196, 2013

19 Borrebaeck CA, "Antibodies in diagnostics-from immunoassays to protein chips" 21 : 379-381, 2000

20 Sin ML, "Advances and challenges in biosensor-based diagnosis of infectious diseases" 14 : 225-244, 2014

21 Ricci F, "A review on novel developments and applications of immunosensors in food analysis" 605 : 111-129, 2007