Phylogeny of Flavobacteria Group Isolated from Freshwater Using Multilocus Sequencing Analysis

다국어 초록 (Multilingual Abstract)

Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we havebeen unable to clearly identify five Flavobacterium species isolated from a freshwater by using the gene as a single marker,because the evolutionary history is incomplete and the pace of DNA substitutions is relatively rapid in the bacteria. In thisstudy, we tried to classify Flavobacterium species through multilocus sequence analysis (MLSA), which is a practical andreliable technique for the identification or classification of bacteria. The five Flavobacterium species isolated from freshwaterand 37 other strains were classified based on six housekeeping genes: gyrB, dnaK, tuf, murG, atpA, and glyA. The genes wereamplified by PCR and subjected to DNA sequencing. Based on the combined DNA sequence (4,412 bp) of the sixhousekeeping genes, we analyzed the phylogenetic relationship among the Flavobacterium species. The results indicatedthat MLSA, based on the six housekeeping genes, is a trustworthy method for the identification of closely relatedFlavobacterium species.

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