Tubulin Polymerization에 대한 Colchicine의 영향 = Effect of Colchicine on the Tubulin Polymerizaiton

다국어 초록 (Multilingual Abstract)

Although the inhibition of microtubule assembly by colchicine is well established, the paradoxical effect of colchicine in the centriole, cilia and basal body is not clear. The microtubule(MADBP^+) was prepared from rabbit brain through 2 cycles of glycerol-and GTP-mediated polymerization and the tubulin without DNA binding protein(MADBP^-) was prepared from MADBP^+ using double strand DNA column chromatography and the properties of these tubulin preparation were compared with those of MADBP^+. The time course of tubulin polymerization was different, with MADBP^+ being sigmoidal and MADBP^- being hyperbolic. MADBP^+ polymerization could be inhibited by colchicine, whereas MADBP^- polymerization could not. There was little difference in colchicine binding activity between MADBP^+ and MADBP^- and the initial binding velocity of MADBP^- was faster than MADBP^-.
From the above results, the possible existence of colchicine sensitive protein among microtubule associated DNA binding proteins was discussed.