손바닥선인장 복합물이 Streptozotocin으로 유발된 당뇨 쥐의 지질대사에 미치는 효과 = Effects of Opuntia ficus-indica Complex on Lipid Metabolism in Streptozotocin-induced Diabetic Rats

다국어 초록 (Multilingual Abstract)

This study was conducted to examine the effects of Opuntia ficus-indica complex (OF) on the lipid metabolism, bile acid in feces, alanine aminotransferase (ALT) activity, aspatate aminotransferase (AST) activity, composition of urine and expression of cholesterol related mRNA in streptozotocin (STZ)-induced diabetic rats. Thirty two male Sprague-Dawley rats were randomly divided into non-diabetic control (NC), diabetic control (DC), diabetic OF of 2% (OF-2) and diabetic OF of 5% (OF-5), then each group was fed for 3 weeks. Plasma total cholesterol, non-esterified fatty acids (NEFA), total cholesterol, low density lipoprotein (LDL), very low density lipoprotein (VLDL) were decreased significantly (p<0.05) in OF-5 group compared to DC, but high density lipoprotein (HDL) was not changed. AST and ALT were also reduced and bile acid excretion was improved. Composition of urine in OF-5 was almost same in NC. The expression of cholesterol 7α-hydroxylase (CYP7A1), 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R), Low density lipoprotein receptor (LDL-R) mRNA indicated that feeding OF have the effects of cholesterol decreation in plasma by synthesis of bile acid from cholesterol. These results provide experimental evidence about improved lipid metabolism of the OF feeding in the STZ-induced diabetic rats.

국문 초록 (Abstract)

제1형 당뇨병이 발병할 경우 지질 대사에 이상이 발생하여 동맥 경화 및 이로 인한 합병증이 발생한다. 지질 대사 이상과 합병증을 개선하기 위해 당뇨에 효과가 있는 것으로 알려진 손바닥선인장을 주재료로 하여 다른 천연물과 배합한복합물(OF)의 지질대사 개선 효과를 알아보았다.
1. OF의 급여는 혈당과 지방 대사를 개선시켜, 혈장 총 콜레스테롤, LDL, VLDL, 중성지방과 유리지방산을 감소시켰다.
2. 간 조직에서 HMG-CoA-R와 LDL-R의 증가를 보였고, 콜레스테롤을 담즙산으로 합성하는 CYP7A1을 증가시켰고, 이는 분변 중 담즙산 함량의 증가와 일치하여 혈장 콜레스테롤이 담즙산의 형태로 배설되어 감소하였음을 확인하였다.
3. OF 급여를 통해 ALT, AST의 감소를 알 수 있었다.
이상의 결과들은 손바닥선인장 복합물인 OF가 제1형 당뇨모델인 STZ으로 당뇨를 유발한 쥐에서 혈중 콜레스테롤을간으로 운반하고, 간에서 답즙산으로 합성하여 배설시켜 혈중 콜레스테롤을 낮췄고, 이 결과로 지질대사를 개선하는 효과가 있음을 보여주었다.

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