The mechanism of NAD^+ photoreduction was studied with chromatophore prepared from a purple bacteria, R. rubrum. The light dependent reduction of pyridine nucleotides was specific for NAD^+ in contrast to the plant system. NAD^+ photoreduction in pur...
The mechanism of NAD^+ photoreduction was studied with chromatophore prepared from a purple bacteria, R. rubrum. The light dependent reduction of pyridine nucleotides was specific for NAD^+ in contrast to the plant system. NAD^+ photoreduction in purple bacteria was affected by the external electron donors such as succinate, TMPDH^-_2 and H_2. A series of high energy compounds could also derive the NAD^+ reduction in chromatophore. the co-presence of ADP-Pi and NAD^+ exhibits mutual inhibition in reduction and phosphorylation. The inhibition of NAD^+ reduction by ADP was completely reversed by DCCD, an energy transfer inhibitor. All the experimental evidences indicate that in purple bacteria, the NAD^+ reduction system and ATP synthetic system share a common high energy intermediate.