중온성 우모 분해균 Bacillus sp. SE-103의 분리 및 배양 조건 최적화 = Optimization of Conditions for Isolating and Cultivating Bacillus sp. Se-103 with a Mesophilic Feather-Degrading Activity

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the possibility to utilize feather meal by bacterial strains. A bacterial strain SE-103 producing keratinolytic enzyme was isolated from the soil of the poultry slaughterhouses. It was identified as Bacillus sp. by judging from its morphological and physiological characteristics. Subsequently the optimal culture conditions for the production of keratinolytic protease by Bacillus sp. SE-103 were investigated. The composition of optimal medium was 3.0% glucose, 0.4% urea, 0.2% $NaNO_3$, and 0.15% KCl. In addition, optimal initial pH and temperature were 6.0 and $35^{\circ}C$, respectively.

참고문헌 (Reference)

1 Sibbald IR, "The utilization of hydrolyzed feather meal by growing chicks" 41 : 844-849, 1962

2 Gregory BR, "Studies on the amino aicd and vitamin composition of feather meal" 35 : 234-235, 1956

3 Rozs M, "Secretion of a trypsin-like thiol protease by a new keratinolytic strain of Bacillus licheniformis" 205 : 221-224, 2001

4 SAS, "SAS System Release 9.1" SAS Institute Inc., Cary, NC. 2002

5 Shih JCH, "Resent development in poulty waste digestion and feather utiliztion - a review" 72 : 1617-1620, 1993

6 Esteban B, "Purification and some properties of an acidic protease from epimastigtes of Trypanosoma cruzi" 77 : 599-604, 1984

7 Shimogaki H, "Purification and properties of a novel surface-activity agent-and alkaline resistant protease from Bacillus sp" 55 : 2251-2258, 1991

8 Xiang L, "Purification and characterization of keratinase from a feather degrading Bacillus licheniformis strain" 58 : 3271-3275, 1992

9 Page WJ, "Phosphoate-mediated alteration of the Microsporum gypseum germination protease specificity for substrate: Enhanced keratinase activity" 117 : 422-431, 1974

10 Ramnani P, "Optimization of medium composition for keratinase production on feather by Bacillus licheniformis RGI using statistical methods involving response surface methodology" 40 : 491-496, 2004

11 Xiang L, "Nucleotide sequence and expressing of keratin, the gene encording a keratinolytic protease of Bacillus licheniformis PWD-1" 61 : 1469-1474, 1995

12 Gassessse A, "Novel alkaline proteases from alkalophilic bacteria grown on chicken feahter" 32 : 519-524, 2003

13 Elaine F, "Keratins and the skin" 11 : 123-153, 1995

14 Chon DH, "Isolation of keratinolytic protease producing microrganism and its cultivation condition" 29 : 134-141, 2001

15 Williams CM, "Isolation idification and characterization of a feather-degrading bacterium" 56 : 1509-1515, 1990

16 Krystyna W, "Intracellular keratinase of Trichophyton gallinae" 25 : 261-268, 1987

17 Pillai P, "Hide depilation and feather disintegration studies with keratinolytic serine protease from a novel Bacillus subtilis isolate" 78 (78): 643-650, 2008

18 Kim JM, "Feather-degrading Bacillus species from poultry waste" 37 : 287-291, 2001

19 Harms RH, "Feather meal in hen nutrition" 36 : 358-361, 1957

20 Suntornsuk W, "Feather degradation by Bacillus sp. FK 46 in submerged cultivation" 86 : 239-243, 2003

21 Amanda SM, "Evaluation of environmental conditions for production of bacteriocin-like substance by Bacillus sp. strain P34" 24 (24): 641-646, 2008

22 Moris WC, "Effect of processing method on utilization of feather meal by broiler chicks" 52 : 866-, 1973

23 Papadopoulos MC, "Effect of different processing conditions on amino acid digestibility of feather meal determined by chicken" 64 : 1729-1741, 1985

24 Rosa SP, "Digestion of keratin Pseudomonas sp" 37 : 1-2, 1996

25 Tideto T, "Degradation of human hair by a thermostable alkaline protease from alkalophilic Bacillus sp" 56 : 1667-1669, 1992

26 Brigitte B, "Charaterization of a keratinolytic serine protease from Streptomyces pactum DSM 40530" 61 : 3705-3710, 1995

27 Macffadin JF, "Biochemical Test for Identification of Medical Bacteria" Boltimore, Maryland 1984

28 John GH, "Bergey's Manual of Determinative Bacteriology" Williams and Wilkins Boltimore, Maryland. 1994

29 Smith RE, "Assessmsnt of the availability of amino acid in feather meal, soybean meal and feather meal by chick growth assay" 47 : 1624-1630, 1975

30 Ryoji T, "A new keratinolytic proteinase from clinical isolates of Trichophyton mentagrophytes" 14 : 506-508, 1987