腸炎 비브리오의 Kanagawa溶血에 關한 硏究 = Studies on the Kanagawa Type Hemolysis of Vibrio Parahaemolyticus

국문 초록 (Abstract)

腸炎비브리菌의 特殊한 溶血現象이라고 報告되어 있는 Kanagawa溶血의 本態를 알고자 몇가지 實驗을 하였다.
海産物에서 分離한 菌의 約 1/3은 陽成反應을 나타내고 約 1/3이 凝陽性이었으나 사람에서 分離한 菌은 모두가 陽性이었다. Kanagawa陽性菌과 陰性菌사이에 溶血에 差異가 있으며 陰性菌에 있어서는 陽性菌에 比하여 溶血이 徐徐히 나타나나 Caion을 加한 培地에서는 陽性菌과 陰性菌 다같이 溶血이 顯著히 促進되었다. Kanagawa溶血現象에 사람과 家兎의 赤血球는 다 같은 結果를 나타냈으며 赤血球의 洗滌 與否도 結果에 影響을 미치지 안하였다. 다면 培地層의 두께는 溶血에는 相當한 영향을 미치며 培地層이 두꺼우면 溶血帶가 적게 나타났다. 개과 고양이에 對한 投與實驗에서 Kanagawa陽性菌과 陰性群사이에 症狀의 出現 및 糞便中 菌排泄에 差異를 볼 수 있다.

다국어 초록 (Multilingual Abstract)

Hemolysis of Vibrio paraaemolyticus on specially prepared media has been consideredd closely reated to its enteropathogenicity and called the Kanagawas phenmoneon. Several reposts in Japan indicate that strains of V. parahaemolyticus isolated from human diarrheal stools are Kanagawa positive, whereas almost all stains isolated from marine specimens are negative. However, this is not well accepted by some workers and reports on food poisonings associated with Kanagawa negative strains are accumulating.
In the study of Kanagawa phenomenon, the author found that a considerably large proportion of this organism isolated from marine specimens was positive for this reaction. The hemolysis on Modified Wagatsuma blood agar by Kanagawa negative strains appeared slowly and became clearly positive at 40 to 49 hrs of incubation. The addition of 0.01 M CaCl_2 in media greatly promoted th ehemoltic activity of Kanagawa negatice strains with a strongly positive reaction at 24 hrs. Many factors influenced the hemolysis by both Kanagawa positive and negative strains, but no qualitative difference was noted. The difference was supposed to be quantitative and to reside in the degree of hemolytic activity. No difference was found between Kanagawa positive and negative strains as the result of feeding experiments in dogs and cats. It was supposed from these results that Kanagawa type hemolysis of V. parahaemolyticus is an expression of strongly hemolytic activity which is distinctly differentiated from the activity of weakly hemolytic strains in special media.