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To investigate circadian rhythm of the phagocytic function of retculoendothelial system of the rats, the phagocytic activity was measured by means of carbon clearance clearance method with 4-h intervals for a 24-h span. The results were as follows. The phagocytic activity increased during the habitual rest light span of nocturnally active rat, but decreased during active dark span. There is no significant difference between male and female rats.