Methylcellulose로 誘發시킨 綱內系 機能變調 白鼠의 免疫機能에 關한 硏究

양동욱 全南大學校 大學院 1980 국내박사

綱內系와 免疫系의 機能的인 關聯性을 追究코자, 白鼠에 methylcellulose를 投與하고 carbon clearance法으로 網內系 機能을 測定하였다. 한편 免疫反應은 細羊赤血球(SRBC)에 對한 溶血素와 凝集素의 力價, 脾臟細胞의 로-젤 形成能 및 足臟腫脹反應檢査로 測定하였으며 組織學的인 檢索을 倂行하여 다음과 같은 結果를 얻었다. 1. MC 投與白鼠에서 綱內系 機能의 低下는 投與量에 比例하였다. 2. 細羊赤血球 對한 體液性 및 細胞介在性 免疫反應 變是 MC 投與量에 比例하여 低下되었다. 3. MC 投與白鼠는 肝腸, 備藏 및 淋巴線等에서 MC를 貧食한 대식구가 增殖되었고 이로 因하여 脾臟과 淋巴線의 旣存 淋巴球 區域은 增殖된 대식구의 結節狀 斑 또는 塊에 依하여 代置되었다. 以上의 成績으로 비추어 MC投與로 誘發시킨 綱內系 機能 項塞은 免疫反應을 低下시키며 그 低下機轉에는 網內系의 機能的인 項塞 自體以外에도 淋巴球의 相對的 消失이 크게 關與함을 示唆해 주었다. This experiment was designed to investigate the effect of functional depression of RES on the immune reactions of rats. The functional depression of RES was achieved by treatments of varying dosages of methylcellulose (MC) during 2 weeks. Degree of the functional depression of RES was evaluated by carbon clearance method. Antigenic challenge was done with sheep red blood cells (SRBC) on the 2nd and 7th days of treatments in rats for the study of humoral immunity and on the last day for the study of cell mediated immunity. Titers of hemagglutinin and hemolysin were measured 24 hours after final treatment by microtitration method. Cell mediated immunity was evaluated by the degree of foot pad reaction of rat with modification of Lagrange's method. Rosette formation of spleen cells to SRBC was also determined. Histological examination of RE-and immune organs was performed. The results obtained were as follows. 1. The administration of methylcellulose depressed the function of rat in proportion to dosage. 2. Titers of hemagglutinin and hemolysin to SRBC decreased and foot pad reaction as well as rosette formation of spleen cells were also decreased in RE-depressed rats in proportion to the administered dosage. 3. The administration of methylcellulose produced market hepatosplenomegaly,and original lymphocytic components in spleen and lymph nodes were by the proliferation of MC-laden macrophages. These observations permit the conclusion that the functional depression of the RES by methylcellulose in rats depress the humoral and cell mediated immunities, possibly due to relative loss of immunocompetent lymphocytes as well as functional blockade of RE-cells.

綱內系가 各種 非 特異的 反應性 肝炎의 誘發에 미치는 影響

서재홍 朝鮮大學校 1984 국내박사

熱傷이 綱內系의 大腸菌 貧食消化能에 미치는 影響에 關한 實驗的 硏究

김인필 漢陽大學校 大學院 1978 국내박사

Rifampicin이 白鼠의 免疫反應 및 綱內系 機能에 미치는 影響

공광찬 全南大學校 大學院 1980 국내석사

本 實驗은 rifampicin이 免疫反應 및 免疫系와 密接한 關聯이 있는 網內系의 機能에 미치는 影響을 觀察하여 rifampicin의 免疫抑制 作用에 미치는 網內系의 役割을 檢討한 바 그 結果는 다음과 같다. 1. Rifampicin은 白鼠의 體液性 및 細胞介在性 免疫反應을 모두 抑制시켰다. 2. Rifampicin의 免疫抑制 反應은 投與量이 增加할 수록 顯著하였으며, 投與後 時日의 經過에 따라 漸次 正常으로 回復되는 傾向이었다. 3. Rifampicin은 免疫抑制 作用과는 對照的으로 網內系 貪食能을 抑制시키지 않았다. 以上의 成績을 要約하며, rifampicin은 子壻의 免疫反應을 抑制하나 網內系 貪食能에서는 別다른 影響을 미치지 않았고, rifampicin에 依한 免疫抑制作用에 網內系 貪食能이 크게 關與할 可能性은 稀薄하다고 思料된다. This experiment was designed for the investigation of the effects of rifampicin on the immune reaction and reticulo-endothelial(RE) function, which seemed to be closely involved in the process of immune reaction and to elucidate the possible role of RE system on the immunosuppression by rifampicin. The rats of different experimental groups were orally received a dose. of rifampicin equivalent to 5, 10, and 20 mg per 1009 of body weight for the successive 10 days. Antigenic challenge was done with sheep red blood cells (SRBC) on the 2nd and 8th days of treatment for the study of humoral immune reaction on the 2nd day of treatment and two days after final treatment for the study of delayed reaction. Rats for the study of cell mediated immunity were antigenically challenged with SRBC on the 8th day of treatment and two days after final treatment. Titers of hemagglutinin and hemolysin were measured 5 days after the second antigenic challenge. Cell mediated immunity was evaluated by the degree of foot pad reaction(FPR) 4 days after antigenic challenge. RE function was measured by the Biozzi's carbon clearance method. The results obtained were as follows. 1. Rifampicin suppressed both humoral and cell mediated immunities in rats. 2. The degree of immunosuppression by rifampicin seemed to be dose dependent, but immunosuppressive effect of rifampicin appeared to recover gradually. 3. Little or no effect of rifampicin was noted on the RE-phagocytic function in rats. From the above, no significant involvement on the phagocytic function of RE-system in the immunosuppression by rifampicin was suggested.

綱內系 貪食能의 日週期 變動에 對한 實驗的 硏究

김흥곤 全北大學校 大學院 1979 국내석사

To investigate circadian rhythm of the phagocytic function of retculoendothelial system of the rats, the phagocytic activity was measured by means of carbon clearance clearance method with 4-h intervals for a 24-h span. The results were as follows. The phagocytic activity increased during the habitual rest light span of nocturnally active rat, but decreased during active dark span. There is no significant difference between male and female rats.

綱內系 塡塞이 四鹽化炭素 肝中毒에 미치는 影響

홍순갑 全南大學校 大學院 1980 국내박사

網內系 塡塞이 四鹽化炭素의 肝 毒性에 미치는 影響을 檢討할 目的으로 著者는 白鼠에 methylcellulose 및 colloidal carbon을 3日間 注入하여 網內系를 塡塞시킨 後 四鹽化炭素를 投與하였다. 實驗群은 methylcellulose 前處置量(0.5%, 1.0% 및 2.0%)과 colloidal carbon 前處置量(3㎎, 8㎎ 및 16㎎/㎖)에 따라 다시 細分하였고, 四鹽化炭素 投與 後 經時的으로 實驗動物을 屠殺하여 白鼠 肝組織에 對한 組織化學的 檢索(SDH, acid-Pase, alk-Pase 및 NS-Est)을 施行하였고, 血淸內 transaminasee를 測定하여 다음과 같은 結論을 얻었다. 1. 網內系 塡塞群에서는 四鹽化炭素 單獨投與群(對照群)에 比하여 四鹽化炭素에 依한 肝細胞損傷을 顯著히 輕減시켰으며, 塡塞劑의 處置量이 많을 수록 그 效果는 著明하였다. 2. 對照群에서는 四鹽化炭素 投與 後 肝細胞內 SDH活性이 減少된 경향을 보인 反面, 網內系 塡塞群에서는 壤死部位를 除外한 肝細胞에서 顯著한 增加를 보였다. 3. 四鹽化炭素에 依한 肝細胞의 形態學的 損傷에 앞서 肝 細胞膜에 存在하는 alk-Pase 活性 變動이 先行함을 알 수 있었다. 4. Acid-Pase 및 NS-Est는 對照群에 比하여 網內系 塡塞群에서 四鹽化炭素 投與 後 肝小葉 周邊部에서 顯著한 增加를 보이며, 特히 acid-Pase는 methylcellulose 前處置보다 carbon 前處置群에서 더 增加된 傾向이었다. This study was designed for the investigation of the possible role of Kupffer cells and sinusoidal lining cell in carbon tetrachloride hepatotoxicity. RES-blockade was achieved by intraperitoneal administrations of varying dosages of methylcellulose or colloidal carbon in successive three days. Single exposure of CC14 was given in control and RES-blocked rats. Changes of transaminase activities were determined following the exposure of CCl₄. Histological and enzyme histochemical studies(H&E, SDH, Acid-Pase, Alk-Pase, and Nonspecific esterase) were also performed. The results obtained were as follows. 1. Transaminase activities and histological examinations revealed that the RES-blockade by methylcellulose or colloidal carbon markedly protected from the hepatotoxicity of CC14. This was more prominent in rats receiving the pretreatments of large dosage of methylcellulose or colloidal carbon. 2. Enzyme activities of succinic dehydrogenase were broadly decreased in centri- and midlobular region of control rats in accordance with degenerative or necrotic area. The decrease of enzyme activities of succinic dehydrogenase, however, was not prominent and restricted in centrilobular region in RESblocked rats, which was followed by increase of enzyme activities later. 3. Generally, histological changes followed enzyme histochemical changes. of alkaline phosphatase activities. 4. Carbon tetrachloride treatments in RES-blocked rats increased the activities of acid phosphatase and nonspecific esterase more than those of control ones in periportal region of hepatic lobule. Enzyme activities of acid phosphatase were far more increased in RES-blocked rats by colloidal carbon than those by methylcellulose.

羊水가 綱內系 貧食能에 미치는 影響

최호선 全南大學校 大學院 1977 국내박사

To evaluate the effect of amniotic fluid on the phagocytic activity of RES, the phagocytic activities were tested by the carbon clearence method in the rats administered with amniotic fluid intraperitoneally, and control fluids such as physiologic saline, ascites, and plasma. The results were as follows: 1. In saline-treated rats, phagocytic activities increased slightly 3 hours after treatment, which were almost normalized 1 day after treatment. 2. Ascites-treated rats showed the moderately increased phagocytic activities 3 hours after treatment, but increased phagocytic activities of the ascites-treated rats returned to the normal control value 1 day after treatment. 3. In the plasma-treated rats, relative increase of phagocytic activities was noted in the 3 hours after treatment, which was followed by marked increase of the phagocytic activities 1 day after treatment. These phagocytic activities were somewhat decreased, but maintained the increased phagocytic activities 5 days after treatment. 4. The amniotic fluid-treated rats showed marked increase of phagocytic activities 3 hours after treatment, which was returned to the normal control value 1 day after treatment.

BCG가 綱內系 機能變調 白鼠에 미치는 形態學的 變化에 關한 硏究

장강원 전남대학교 대학교 1982 국내박사

Although BCG is the most widely used immunostimulatory agent in the immunotherapy of malignant diseases, only limited information is available about BCG-induced morphological alteration of RES. It has been well known that though both zymosan and methylcellulose are stimulants to the reticuloendothelial cell proliferation, zymosan stimulates the phagocytic activity, while the activity is depressed by methylcellulose. The purpose of this study is to determine the effect of BCG on the morphological alteration of RES submitted to methylcellulose or zymosan. Experimental groups are divided into the following; BCG-treated, zymosan-treated, methylcellulose-treated, BCG-zymosan-treated, and BCG-methylcellulose-treated. The experimental rats of each group were sacrificed at 4, 8, 12 and 16 weeks intervals, and the liver, spleen and lung are studied by the histological and enzyme histochemical methods. The results are as follows. 1. BCG-treated group showed systemic proliferation of macrophages and multifocal infiltration of pyroninophilic lymphocytes involving the portal areas of the liver, the white and red splenic pulps, and the bronchial lymph nodes. These reactions reached a peak at 4th and 8th weeks. The BCG granuloma was observed only in the lung at 12th week. Increased activity of acid phosphatase was noted in the liver, spleen and lung. 2. Zymosan-treated group showed systemic proliferation of macrophages forming numerous nodules, in which phagocytosed zymosan was contained. The involved sites were the Kupffer cells of the liver, the marginal zone and red pulp of the spleen, the alveolar walls of the lung, and the bronchial lymph nodes. A slight increase of the acid phosphatase activity was observed in all organs tested. 3. In the methycellulose-treated group, diffuse proliferation of macrophage was observed in the liver and lung, while the nodules containing phagocytosed methycellulose were marked only at the marginal zone and red pulp of the spleen. No significant changes of the enzyme activities tested were noted. 4. In the BCG and zymosan-treated group, the cellular proliferation and the phagocytic nodule formation seemed to be more pronounced than those of BCG-treated or zymosan-treated groups. Also there was marked elevation in the activities of the acid phosphatase, alkaline phosphatase and nonspecific esterase. 5. In the BCG and methylcellulose-treated group, the proliferation of macrophages, phagocytic nodule formation in both spleen and lung, as well a~ the increase in all enzyme activities were more marked than those of methylcellulose-treated group. From these results, it may be concluded that BCG enhance the phagocytic function of the reticuloendothelial cells submitted to zymosan or methylcellulose.

Stretozotocin 糖尿病 白鼠의 綱內系 機能에 關한 硏究

설용환 전남대학교 대학원 1977 국내석사

To study the influence of diabetes mollitus on the stiouloendotholial function, the phagocytic activity of he reticuloendothelil system and fasting serum glucose evel were assesed in the streptozotooin-induced diabatic ats. The results were a follows. 1. Fasting serum glucose level showed marked increase and 4 hours after the administration of streptozotoin(STZ). In 7 and 10 hours after the administration, the hagocytic activity was markedly decreased to the hypoglyomic level. Thereafter, however, it soon increased above he normal level and the hyporglycemic condition was aintained throughout the experimental period, 1 week. 2. The phagecytic activity of the reticuloendothelialystom increased slightly 1 and 4 hour after the administration of STZ. After the transient decrease in 7 and 10 hours, the phagocytic activities were markedly increased in 1 and 7 days after injection of STZ. 3. There results suggest that the early changes of the phagocytic activity within 24 hours after STZ administration have direct causal relationship with the changes of the serum glucose level. The changes after 24 hours, otherwise, seemed to reflect the secondary metabolic adjustments due to the hormonal factors, deranged carbohydrate and other fcedstuff metabolism.

部分的 肝切除 後의 鋼內系 機能에 關한 硏究

소흥영 전남대학교 1973 국내박사