WHO 고령친화도시 인증 및 조성을 위한 지역정책 개선방향 연구

허윤정 을지대학교 대학원 2025 국내박사

본 논문은 초고령사회로 빠르게 전환되고 있는 우리나라의 지역사회 인구구조 및 대내외 사회변화의 선제적이고 효율적인 대응과 함께 농촌 등 지역사회가 글로벌 고령친화 지역으로 거듭나기 위한 방안을 제시하는 논문이다. 급속한 시니어(시니어)의 증가는 한국의 커뮤니티 케어 서비스, (장기)요양보호, 의료사각지대 및 복지 취약지역 개선이 지역사회 차원에서 필수적으로 지원되어야 할 현상과 맞물려 전반적인 사회 인구구조 변화 및 국내외 환경변화를 고려하기 위한 중앙 및 지방 정부의 전반적인 정책 및 제도 정비가 필요한 시점이다. 이에 따라 WHO가 제시한 고령친화도시의 8대 가이드라인을 기반으로 한국 지역사회 현황에 적합한 고령친화도시 구축을 위해 먼저 세대별 인식 차이에 대한 확인이 필요하다. 본 논문은 노인친화환경을 지역 정주 환경, 사회문화 참여환경, 사회서비스 요인 등으로 분류하고 각각의 세부요인이 삶의 질에 미치는 이유를 세대 및 지역별로 살펴본다. 또한 점점 깊어지는 초고령사회에 대한 정책을 마련하는 것이 목적이다. 노인친화환경의 세 가지 요인과 각 세대별, 지역에 대한 다양한 정책의 필요성 및 세부 요인을 분석하기 위해 집단 및 회귀분석을 통해 살펴보았다. 결과적으로 거의 모든 연령 및 지역에서 노인친화환경이 삶의 질과 만족도에 영향을 미쳤으나 세대별 영향을 미치는 변수의 범례에는 어느 정도 유의미한 결과가 나타났다. 39세미만의 세대에서는 지역의 거주지 환경 및 편의성이, 그리고 40세이상 59세 미만 및 60세이상 노년층에서는 사회 서비스 환경과 지역의 거주지 환경 및 편의성이 통계적인 의미가 있는 것으로 나타났다. 이러한 결과는 노인친화환경은 시니어뿐만 아니라 전 세대의 거주지역의 삶의 만족도에 영향을 준다는 것이 검증되었으며, 이러한 수치를 통해 다음과 같은 결과를 표현하였다. 첫째, 지역사회 시니어 주거 환경의 개선, 둘째, 지역사회 교통체계의 개선, 셋째, 시니어의 사회관계망 지속에 대한 정책적 노력 등이 필요한 것으로 나타났다.

갑상선기능이상 환자에서 크레아티닌과 시스타틴을 이용한 사구체여과율 검사결과의 상관성 연구

임구 을지대학교 대학원 2017 국내석사

갑상선기능이상 환자에서 혈청 크레아티닌과 시스타틴을 이용한 사구체여과율 검사결과의 상관성 연구 임구, 성호중 을지대학교 시니어헬스케어학과 Correlation Analysis of Serum creatinine based eGFR and Serum cystatin C based eGFR with Thyroid dysfunction patients. Gu Lim, Hojung Sung Department of Senior Healthcare, College of Health Science, Eulji University, Seongnam, Korea, Abstract Background : serum creatinine based eGFR and serum cystatin C is the most popular method for measurement of renal function. thyoid hormone is known to affect serum creatinine based eGFR and serum cystatin C based eGFR, however, the clinical significance of thyoid dysfunctional patients of renal function evaluation is yet to be completely elucidated. this study examined the effect of thyroid hormone on serum creatinine based eGFR and serum cystatin C based eGFR in patients with thyroid dysfunction. Method : 442 patients with hypothyroidism. 284 patients with hyperthyroidism. 35 patients with other thyroid disease. Correlation analysis between thyroid hormone and serum creatinine based eGFR, serum cystatin C based eGFR. Results : Correlation analysis between thyroid hormone and serum cystatin C based eGFR is indicated that serum cystatin C based eGFR is more independent biomarker than serum creatinine based eGFR with thyroid dysfunction patients. Conclusion : serum cystatin C based eGFR is more accurately reflect renal function than serum creatinine based eGFR with thyroid dysfunction patients.

A feasibility study of a visible/NIRF/gamma multi-modal laparoscope with SiPM-based gamma detection

Han, Young Been Graduate School, Eulji University 2019 국내석사

Recently, the cancer therapy has been greatly developed for radiation and chemotherapy method. However surgical resection is recommended for the majority of cancers. For this reason, intraoperative imaging study has emerged as surgical tools for identifying tumor margin. Intraoperative imaging has been studied using conventional image devices. The optical NIR image was acquired from fluorophore, and gamma and ultrasound image were acquired by using radioisotope tracer and ultrasound probe, respectively. However, each single modality has many limitations such as depth penetration, and spatial resolution. To overcome this limitation, multi-modality intraoperative imaging studies are ongoing. In multi-modality image, the gamma image was acquired by using CCD camera collecting scintillation photons from crystals. The CCD-based gamma image is difficult to evaluate gamma energy quantitatively. In this study, a multi-modal laparoscope was constructed. The SiPM-based gamma detector was constructed by using GATE Monte-Carlo simulation. The visible and NIR detectors consist of GRIN lens and CCD camera, respectively. The multi-modal laparoscope was used to evaluate the intrinsic performance of the gamma detector and simultaneous imaging was obtained. In future works, small animal experiment will be performed for the feasibility study of the multi-modal laparoscope.

Discovery of the internal standard metabolites for volume estimation in bloodstains

Lee, Seung Yeon 을지대학교 대학원 2022 국내석사

Bloodstains generated by blood leakage are often found at crime scenes and are valuable evidence for reconstructing the crime scene. Quantitative information on changes in bloodstain components over time can be used as an index for estimating the elapsed time of bloodstains. In addition, the physical information associated with the pattern of bloodstains is used to infer possible bloodshed situations. Bloodstains are affected by various environmental factors in the external environment and their volume changes during the drying process. The estimation of the elapsed time of bloodstain is based on quantifying and comparing quantitative changes in bloodstain components between time points and time points. In order to compare quantitative changes in bloodstain components, it is necessary to first check whether the results are quantitative results that depend on the volume of the initial blood state of bloodstains. Although there have been several attempts to estimate blood origin in bloodstain pattern analysis, studies on estimating blood origin volume in quantitative analysis of blood components are extremely rare. Therefore, in this study, metabolites were found that maintain a constant concentration over time in bloodstains prepared in various volumes. Six metabolites showing CV values of less than 20% between samples in the same volume within over time, and whose concentration increased in proportion to the volume increase in the source blood, were discovered as internal standard metabolites, and through the online database three MFs were identified.

Protective Effects of Oryza sativa and Gastrodia elata Blume on Ovalbumin-Induced Lung Inflammation in Rat Allergic Asthma Model

Park, Yu Rim Graduate School, Eulji University 2019 국내석사

Allergic asthma is a chronic lung disease caused by inhalation of external allergens. Symptoms such as dyspnea, cough, wheezing, chest tightness and sputum appear caused by abnormalities of bronchial smooth muscle. The incidence of allergic asthma in recent 20 years has been steadily increased, especially among children. Oryza sativa (OS) and Gastrodia elata Blume (GEB) are known to have excellent anti-inflammatory effect and are attracting attention as a health functional food. In this study, we investigated the protective effects of OS and GEB extracts in allergic asthma model induced by ovalbumin (OVA) in rats. Rats were randomly divided into five groups; ⅰ) negative control group, no administration during the experimental period, ⅱ) control (CON) group, ⅲ) OVA group, ⅳ) OS group and ⅴ) GEB group. Rats of OVA, OS and GEB groups were sensitized by intraperitoneal injection of 0.3 mg OVA into 1 ml saline containing 30 mg aluminium hydroxide (alum) on every other day for 14 days. The CON group received 30 mg alum into 1 ml saline. OVA-sensitized rats were challenged with 2% OVA in saline (50 µl) by intranasal inoculation on each side of the nose from days 29 to 35. The CON group was received only saline. The OS and GEB groups were received OS and GEB extracts (10 ml/kg, oral) from days 15 to 35 once daily. The CON and OVA groups were received distilled water (10 ml/kg, oral). All rats were sacrificed 24 h after the last administration. Level of total-IgE, the number of eosinophils and thickness of alveolar septa were decreased in the OS and GEB groups compared with the OVA group. In hematoxylin and eosin and Sirius red staining, pulmonary edema, infiltration of inflammatory cells, fibroblast proliferation and collagen deposition were decreased in the OS and GEB groups compared to the OVA group. In immunohistochemistry, expression of interleukin-1 beta (IL-1β), IL-4, IL-5, myeloperoxidase and CD206 was significantly decreased in the OS and GEB groups compared to the OVA group. In conclusion, OS and GEB extracts showed protective effects in OVA-induced allergic asthma rats by reduction of infiltration of inflammatory cells and macrophages. 알레르기성 천식은 외부 알레르겐의 침입으로 인해 기관지가 좁아지면서 호흡 곤란, 기침, 천명, 흉부 압박감, 객담 등과 같은 증상이 나타나는 만성 폐질환이다. 최근 20년간 알레르기 천식의 발병률은 특히 어린아이에게서 꾸준히 증가하고 있어 이에 따른 관심이 고조되고 있다. 흑미와 천마는 항염증, 항산화 효과가 뛰어난 것으로 알려져 있어 건강기능식품으로 주목 받고 있다. 본 연구에서는 난백 알부민 (OVA)으로 유도한 알레르기 천식 모델에서 흑미와 천마 추출물의 보호효과를 검색하기 위해 실시하였다. 랫드를 무작위로 5개의 군으로 나누었다. i) 실험 기간 동안 아무것도 투여하지 않은 음성 대조군, ii) OVA 감작을 하지 않고 21일 간 증류수 (10 ml/kg)를 경구 투여한 대조군 (n=5). iii) OVA 감작을 하고 증류수 (10 ml/kg)를 경구 투여한 천식군 (n=5). ⅳ) OVA 감작을 하고 흑미 추출물(10 ml/kg)을 경구 투여한 흑미군 (n=5). ⅴ)OVA 감작을 하고 천마 추출물 (10 ml/kg)을 경구 투여한 천마군 (n=5). 마지막 투여 24 시간 후에 졸레틸로 마취하여 희생시켰다. 혈청학적 방법을 통해 총 혈청 IgE를 측정하였다. 조직학적 변화 관찰을 위해 헤마톡실린-에오신, 시리우스 레드 염색을 진행하였고 알레르기 천식 관련 사이토카인의 발현을 면역조직화학염색으로 관찰하였다. 염증 세포의 침윤, 호산구 수, 폐포 중격의 면적은 대조군에 비해 천식군에서 증가하였고 흑미군과 천마군에서 감소하였다. 혈청의 총 IgE 수치는 천식군에 비해 흑미와 천마군에서 감소하였다. 조직병리학적 변화를 관찰한 결과, 천식군에서 폐 부종, 폐포 중격의 두께 증가 및 염증 세포의 침윤, 섬유모세포 증식, 콜라겐 섬유의 축적이 나타났다. 반면 흑미와 천마군에서 이러한 변화는 감소되었다. 또한, 면역조직화학염색에서 IL-1β, IL-4, IL-5, MPO, CD206의 발현이 대조군에 비해 천식군에서 유의적으로 증가 하였고 흑미와 천마군에서 감소하였다. 따라서, 본 연구에서 흑미와 천마 추출물의 투여가 염증세포와 대식세포의 침윤을 감소시킴으로써 OVA으로 유도한 알레르기 천식 모델에 대한 보호효과를 가진다는 것을 확인하였다.

Fisetin protectes H9c2 cardiomyoblast cells against H2O2induced apoptosis through Akt and ERK1/2 signaling pathways : Evaluation of cardiac muscle cell therapy efficacy of fisetin

Lee, Jeong Soo Graduate School, Eulji University 2018 국내박사

Fisetin (tetrahydroflavone), a nutral component of plants, is known to have direct radical scavenging, anti-inflammation and cell survival properties. This study investigated whether fisetin can protect myocardial cells against cell death induced by H2O2, which is associated with cardiovascular risk. The effects of fisetin on the expression of antioxidant proteins such as superoxide dismutase (SOD, Cu / Zn-SOD and Mn-SOD), catalase (CAT), and heme oxygenase (HO-1) in H9c2 cells exposed to H2O2 were analyzed by western blot Regulation of the enzyme involved in the removal of fisetin during to H2O2-induced oxidative stress resulted in an increase in Cu / Zn-SOD expression compared to that in cells treated with H2O2 alone at a concentration of 5 and 10 μM . However, HO-1, Mn-SOD and CAT expression was not increased after treatment with fisetin. These data indicate that fisetin reduces oxidative damage by enhancing the antioxidant capacity associated with Cu / Zn-SOD in cell. The enzyme can be rapidly a first line of defense when cell are exposed to oxygen-derived free radicals or organisms are under oxidative stress. These results indicate that fisetin protects H9c2 cells treated with H2O2 from reactive oxygen species (ROS) produced during oxidative stress and enhances antioxidant enzyme activity. Fisetin reduced H2O2-induced apoptosis in H9c2 cells, Further fisetin clearly showed cardioprotective effects and enhanced cell survival, suggesting that recovery of mitochondrial energy played a role in its protection. We therefore assessed the effect of fisetin on cell death by FACS analysis after double staining with annexin-V and propidium iodide. Survival increased in a concentration-dependent manner. relative to that in the control group, where H2O2 treated H9c2 cells were not exposed to fisetin, The effect of fisetin on the expression of apoptotic regulatory proteins including caspase-3, (Bax, and Bcl-2) in H2O2-stimulated cells was examined to confirm the anti-apoptotic effect of fisetin in H2O2-treated cells. Bcl-2 expression decreased after H2O2 treatment, whereas H2O2 treatment significantly increased the expression of cleaved caspase-3 and Bax compared to that in the control cells (without H2O2 treatment). Fisetin pretreatment significantly reduced both H2O2-induced Bax levels in cleaved caspase-3 and H9c2 cells. In addition, H2O2 treatment reduced the ratio of Bcl-2 to Bax compared with the normal control, whereas fisetin treatment induced recovery of the Bcl-2-/-Bax ratio in a dose-dependent manner. Fisetin increased protein kinase B (Akt) and extracellular regulated kinase (ERK) 1/2 phosphorylation. We examined signaling pathways that might, support the protective effect of fisetin against cell damage induced by H2O2. Activation of the PI3K-/-Akt pathway in H9c2 cells inhibits apoptosis and promotes cell survival, and mitogen-activated protein kinases (ERK1 / 2), including MAPKs, are involved in the regulation of apoptosis. Therefore, we examined whether the P13K/AKT pathway could be altered by fisetin in H2O2-exposed cell. H9c2 cells were treated with H2O2 for 30 minutes and then the expression and phosphorylation of Akt, ERK1-/-2 and glycogen synthase kinase (GSK) -3β were measured. Phosphorylation of Akt and ERK 1/2 were dramatically increased in a concentration-dependent manner after H9c2 cells exposed to H2O2 were treted with fisetin. However, no significant change in GSK-3 b type phosphorylation was observed after fisetin treatment of H2O2-exposed H9c2 cells. These results indicate that the Akt and ERK1-/-2 signaling pathways may be involved in the anti-apoptotic effects of fisetin in H9c2 cells exposed to H2O2 in particular. Inhibition of the Akt and ERK1-/-2 signaling pathways plays an important role in the regulation of apoptosis in H2O2-exposed H9c2 cells. H9c2 cells were pretreated with LY294002 (a PI3K / Akt pathway inhibitor, 20 μM) and PD98059 (an MEK inhibitor, 20 μM) and fisetin (10 μM to determine whether increased Akt and ERK1-/-2 phosphorylation contributeds to the cardioprotective effects of fisetin). In the absence or presence of H2O2, neither LY294002 nor PD98059 did affected cell viability. However, pre-treatment with LY294002 and PD98059 reduced the protective effect of fisetin in cell exposed to H2O2. When cells were pretreated with LY294002, PD98059 and fisetin the viability of the cells exposed to H2O2 decreased compared to that of cells treated with fisetin alone. It was This was further analyzed by Western blotting with. The effects of LY294002 and PD98059 decrease Akt and ERK1-/-2 phosphorylation, respectively by western blot analysis. Fisetin pre-treatment decreased both Akt and ERK1-/-2 the LY294002 and PD98059. treatment group. Also, LY294002 및 PD98059는 caspase-3, Bax, In addition, LY294002 and PD98059 reversed the protective effect of fisetin in H2O2 exposed cell and increased antioxidant enzyme activity as determined by the expression of apoptosis-related proteins such as cleaved caspase-3, Bax and Bcl. The, he data suggest that the protective action of against H2O2-induced death of H9c2 cells is at least in part mediated by the Akt and ERK1 /-2 signaling pathwasys. Fisetin increased the phosphorylation of apoptotic cells and intracellular ROS, while reducing the expression of Cu-/-Zn-superoxide dismutase (SOD and Akt cell kinase (ERK) 1/2 Let. In particular, fisetin significant reduction apoptosis by inhibiting cleaved caspase-3 and Bax-, and increased the anti-apoptotic activity of Bcl-2 the enzymes in H9c2 cells. However, heme oxygenase, Mn-SOD and catalase were not changed by fisetin under these conditions. Taken together, these data suggest that, the potential cardioprotective effects of fisetin may be dependent on Akt and Cu-/-Zn-SOD-mediated activation of Bcl-2 via the ERK1 / 2 pathway. Therefore fisetin could be useful in the treatment of myocardial cells caused by ischemia / reperfusion.

Mechanism of Hinokitiol Induced Autophagy and Apoptosis in Cancer Cells

Lee, Taebok Graduate School, Eulji University 2019 국내박사

Cancer is genetically, metabolically and infectiously induced life threatening disorder showing aggressively growing pattern with invasive tendency into the neighboring tissue and organs. In order to prevent this global menace from jeopardizing human life, enormous studies on carcinogenesis and cancer therapeutic agents with chemotherapy resistance have been intensively researched. Hinokitiol (β-thujaplicin) extracted from heart wood of cupressaceous is a well-known bioactive compound demonstrating anti-inflammation, anti-bacteria and anti-cancer effects on several cancer types via apoptosis and autophagy. This study proposed that hinokitiol activates transcription factor EB (TFEB) nuclear translocation for autophagy and lysosomal biogenesis regardless of nutrient condition in cancer cells. Mitochondria segmentation and β-catenin translocation into the nucleus under treatment of hinokitiol on non-small cell lung cancer (NSCLC) cells and HeLa cells were investigated. Hinokitiol exerted cytotoxicity on HeLa, HCC827 and gefitinib (Iressa) resistant HCC827 cells (HCC827-GRKU); moreover, artificially induced autophagy by overexpression of TFEB granted imperfect sustainability onto HeLa cells against hinokitiol. Taken together, hinokitiol is the prominent autophagy inducer and activator of TFEB nuclear translocation. Alternative cancer therapy via autophagy is pros and cons since the autophagy in cancer cells is related to prevention and survival mechanism depending on nutrition. To avoid paradox of autophagy in cancer therapy, fine-tuned regulation and application of hinokitiol in due course for suppressing cancer cells are recommended. Also, this study suggests that the anticancer effect of hinokitiol was reinforced by combination therapy with curcumin on HCC827 and HCC827-GRKU cells. The synergistic anticancer effect of co-treatment of curcumin and hinokitiol on HCC827 and HCC827-GRKU cells was the first evidence elucidating the alternative application of fine-tuned combination therapy on cancer cells with curcumin and hinokitiol.

A multimodal laparoscope for simultaneous NIR/gamma/visible fusion imaging

Kang, Han Gye Graduate School, Eulji University 2018 국내박사

Intraoperative near-infrared fluorescence (NIRF) guided laparoscopic surgery using indocyanine green (ICG) has been used widely to identify the sentinel lymph node (SLN) of prostate cancer patients. However, NIRF imaging is limited by the tissue penetration depth of less than 10 mm. In contrast, intraoperative gamma imaging of 99mTc-nanocolloid can identify the SLN located deep in the tissues. Moreover, preoperative SPECT/CT images of 99mTc-nanocolloid can be used as reference data during laparoscopic surgery. Recently, hybrid surgical technique using an optical/nuclear hybrid tracer ICG-99mTc-nanocolloid, has been developed and verified in clinical trials by European researchers. Nevertheless, no multimodal laparoscopic NIR/gamma/visible imaging system has been developed yet. For these reasons, we have developed a novel prototype multimodal laparoscope which can provide NIR/gamma/visible fusion images simultaneously for SLN mapping by using a wavelength division multiplexing method. The performance of the proposed multimodal laparoscope was evaluated using various phantom studies. The gamma imaging could complement the depth limit of NIR imaging, while NIR imaging provides high resolution fluorescence image in real-time. The proposed multimodal laparoscope can take advantages of the deep penetration capability of gamma imaging (140 keV), and high resolution of NIR/visible imaging. In conclusion, we first demonstrated the feasibility of a novel multimodal laparoscope which is capable of simultaneous NIR/gamma/visible imaging for SLN mapping using a hybrid ICG-99mTc-nanocolloid radiopharmaceutical. 목적 : 근적외선 형광 (Near infrared fluorescence: NIRF) 유도 복강경 수술은 전립선 암에서 감시림프절 (Sentinel lymph node: SLN) 생검 및 절제를 위해 전세계적으로 활발히 사용되어오고 있다. 하지만 대표전인 근적외선 형광물질인 인도시아닌그린(ICG)에서 방출되는 근적외선 형광의 조직 투과도가 10 mm 이하로 제한된다는 단점이 있어서 조직 깊은 곳에 위치한 감시림프절을 탐지 못 하여 위음성률이 높아질 수 있다. 반면에 방사성의약품인 99mTc-nanocolloid를 이용한 핵종유도 수술은 조직 깊은 곳에 위치한 감시림프절도 탐지할 수 있고 수술 전에 획득한 SPECT/CT 영상으로부터 감시림프절의 위치를 미리 파악할 수 있다는 장점이 있다. 때문에 최근 유럽에서는 근적외선 형광물질과 방사성의약품이 결합된 다중 추적자인 ICG-99mTc-nanocolloid를 이용하여 감시림프절 탐지의 민감도와 특이도를 높이는 수술법을 개발하였다. 하지만 근적외선 형광과 감마선 영상을 하나의 장치로 동시에 검출할 수 있는 장치는 개발되지 않았기 때문에 근적외선 형광 복강경과 감마선 프로브를 개별적으로 사용해야 한다는 단점이 있다. 따라서 본 연구에서는 근적외선 형광 영상, 감마선 영상, 가시광선 영상을 하나의 장치로 획득할 수 있는 융합 복강경을 개발하여 다중추적자인 ICG-99mTc-nanocolloid를 이용한 복강경 수술 시 감시림프절 탐지의 민감도와 특이도를 높이고자 한다. 새로 개발된 융합 복강경의 성능은 다양한 팬텀 실험을 통해 정량적으로 평가되었다. 융합 복강경의 감마 영상은 투과력이 약한 근적외선 형광 영상을 보완할 수 있었고 실시간 고분해능의 근적외선 형광/가시광선 영상을 이용하여 감시림프절 팬텀을 탐지할 수 있었다. 추후 본 연구에서 개발된 융합 복강경의 임상적 유효성을 동물 실험을 통하여 검증할 계획이다.

The Preventive Effect of Gastrodia Elata Blume Extract on Vancomycin-induced Acute Kidney Injury in Rats

LEE, YEON-SU 을지대학교 대학원 2021 국내석사

One of the components in Gastrodia elata Blume (GEB) extract is p-hydroxybenzyl alcohol, which inhibits reactive oxygen species (ROS) production, exhibits anti-inflammatory activity, and downregulates the free radical and lipid peroxide levels. Vancomycin hydrochloride (VAN), a glycopeptide antibiotic, is associated with several side effects, including nephrotoxicity. This study investigated the preventive effects of GEB extract on vancomycin-induced acute kidney injury in rats. Five-week-old male Sprague-Dawley rats were randomly divided into the following three groups: control (CON) group, orally administered distilled water (10 mL/kg BW); vancomycin (VAN) group, orally administered distilled water (10 mL/kg BW); Gastrodia elata Blume (GEB) group, orally administered GEB extract (10 mL/kg BW). The treatment period was 14 days. The VAN and GEB groups were intraperitoneally administered VAN (400 mg/kg BW) after oral administration for the last 3 days of the 14-day treatment period. The rats were anesthetized using isoflurane at 24 h after the last VAN administration and sacrificed. The kidney weight and the serum levels of blood urea nitrogen and serum creatinine levels in the GEB group were significantly lower than those in the VAN group. Histological analysis using hematoxylin & eosin and periodic acid Schiff staining revealed pathological changes in the renal section, such as tubular damage and basement membrane damage of the VAN group, which were mitigated in the renal section of the GEB group. Immunohistochemical analysis revealed that the expression levels of N-acetyl-D-glucosaminidase, myeloperoxidase, and tumor necrosis factor-alpha in the GEB group were decreased when compared with those in the VAN group. Compared with that in the VAN group, the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells was significantly lower in the GEB group. The levels of total glutathione, an antioxidant, in the GEB group were significantly higher than those in the VAN group. Compared with those in the VAN group, the malondialdehyde levels were lower in the GEB group. The findings of this study suggested that GEB extract prevents VAN-induced renal tissue damage by exerting antioxidant and anti-inflammatory effects.

불임 검사용 여성호르몬 5종에 대한 효소 화학발광 면역법 개발

김성수 을지대학교 대학원 2021 국내석사

현재 난임 부부는 지속적으로 증가하고 있고, 난임 시술에 따른 비용도 상승하여 심각한 경제적 부담이 되고 있어, 이를 해결하기 위해 국가적 차원의 ‘난임부부 시술비 지원사업’이 이루어지고 있다. 최근 난임 부부에 대한 건강보험을 적용해 난임 부부들이 시술을 받을 때 드는 검사비, 마취비, 약제비 등 비용 부담을 건강보험에서 지원 되어, 건강보험이 적용되면서 검사비용에 대한 부담이 감소해 난임 검사 수가 증가하고 있어 본 기술의 개발이 반드시 필요하다고 할 수 있다. 여성 난임의 원인이 매우 다양하므로 그 원인을 찾기 위한 검사로 초음파검사, 호르몬검사, 자궁-난관 조영술, 성교후검사, 자궁내막검사 등을 시행하고 있다. 호르몬 검사는 여성 난임 진단을 위한 간단하고 효율적인 방법으로 간주되어 왔다. 난임 진단을 위한 호르몬 검사에 이용되는 5가지 호르몬은 Luteinizing hormone (LH), Follicle-stimulating hormone (FSH), Estradiol(E2), Progesterone, Anti-Mullerian hormone (AMH)으로 혈액 내에 존재하는 양을 측정하는 방식이다. 이에 본 기술 개발은 주요 여성호르몬 5가지를 Chemiluminescence enzyme immunoassay(CLEIA)측정 시스템을 통하여 측정한다. CLEIA 측정 시스템에 경우 ELISA 시스템과 마찬가지로 5종류의(Direct CLEIA, Indirect CLEIA, Sandwich CLEIA, Competitive CLEIA, Bridge type CLEIA) 시스템으로 구성 할 수 있으며, direct CLEIA와 indirect CLEIA는 측정 방식의 차이로 CLEIA를 구분하는 방식이며 감도 때문에 주로 indirect CLEIA 측정방식을 많이 사용한다. 5 가지 호르몬 중 LH, FSH 및 AMH는 sandwich CLEIA 분석법을 기반으로 하고 다른 호르몬인 E2 및 progesterone은 competitive CLEIA 분석법을 기반으로 하였다. 경쟁 반응을 위해, 서로 다른 용량의 E2 및 progesterone이 micro well plate의 호르몬 분석 물에 결합 된 고정 된 담체 단백질 (예 : 소 혈청 알부민)과 경쟁적으로 반응했다. 이 연구에서 개발된 CLEIA 시스템은 분석 감도 (2 ~ 5배 더 높음) 및 분석 시간 (60분 이내) 측면에서 상업용 비색 ELISA 키트보다 우수하여 빠르고 저렴한 불임 호르몬 검사가 가능하다. 각 호르몬에 대한 재현성은 분석 평가에서도 허용 되었다. (분석 간 및 분석 내 분산 계수는 각각 20 % 및 10 % 이내였다). 또한, 우리는 현재 이 분석 플랫폼을 자동 액체 처리 시스템과 결합하여 높은 처리량의 결과와 재현성을 높이고 있다.