원거리 플라즈마 화학증착법으로 증착된 이산화규소박막의 물성

박영배,강진규,이시우,Park, Yeong-Bae,Gang, Jin-Gyu,Lee, Si-U 한국재료학회 1995 한국재료학회지 Vol.5 No.6

Silicon oxide thin films were deposited by remote plasma chemical vapor deposition (RPCYD). The effect of the operating variables, such as plasma power, deposition temperature and partial pressure of reactant on the material Properties of the silicon oxide film was investigated. By XPS, it was found out that the film was suboxide (O/Si<2) and small amount of nitrogen due to the plasma excitation was accumulated at the Si/SiO$_2$interface. The amount of dangling bonds at the Si/SiO$_2$interfaces were measured by ESR and the concentration of hydrogen bond was obtained by SIMS and FT-IR. The bond angle distribution(d$\theta$/$\theta$) was shown to be similiar to thermal oxide above 20$0^{\circ}C$ but the etch rate was higher than that of the thermal oxides due to the structural difference and the stress between silicon substrate and silicon oxide film.

PCR과 SDS-PAGE를 이용한 Bifidobacteria의 동정

박시홍,송희성,이지연,김해영 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-

SDS-PAGE를 이용한 단백질 패턴의 분석결과, 같은 종의 Bifidobacterium은 동일한 단백질 패턴을 보이며, 다른 종의 Bifidobacterium은 서로 다른 패턴을 보이고 있는 것을 확인하였다. 따라서 SDS-PAGE를 이용한 whole cell protein 패턴 분석으로 Bifidobacterium을 간편하고 빠르게 동정할 수 있는 가능성을 보였다. DNA homology 98% 이상으로 PCR에 의해 동정에 어려움이 많았던 B. catenulatum, B. Pseudocatenulatum group, B. animalis을 SDS-PAGE로 확인한 결과, B. catenulatum과 B. pseudocatenulatum, B. animalis는 뚜렷한 단백질 패턴의 차이를 보임으로써 구별이 가능했다. PCR 수행 시 primer제작은 기존에 보고된 것과 NCBI blast program을 통하여 제작을 하였으며, 그 결과 B. longum, B. bifidum, B. adolescentis 3종류의 종에서만 특이적으로 band가 나타나는 것을 확인할 수가 있었다. 본 실험에서는 SDS-PAGE와 PCR을 이용한 균주 동정법으로 신속하고 정확하게 Bifidobacterium을 탐색할 수 있었으며, 발효유 제조나 의약품 제조 등 산업적으로 이용 될 수 있을 것이다 (Mitsuoka, 1984). Bifidobacterium is a Gram-positive obligate anaerobic bacterium which is able to change cellular shape by nutritional conditions and dominant species in intestinal microflora of human. In order to identify Bifidobacteria spp. in human intestine, PCR and SDS-PAGE of whole cell proteins were used. Species-specific primer sets based on the 16S rRNA, 16S-23S intergenic spacer and heat shock protein genes of three Bifidobacteria spp. were constructed using NCBI blast program (Bifidobacteria adolescentis, B. longum and B. bifidum). PCR products using each primer sets showed the same sizes of expected target genes. From the SDS-PAGE of whole cell proteins in bifidobacteria, profiles of SDS-PAGE between 13 type strains showed distinct differences. These methods will be useful for monitoring and evaluating Bifidobacteria spp. in human intestine microbial population.

방전관용 전극개발 및 특성에 관한 연구

안성환,황동원,최준영,송시준,전용우,박원주,이광식 영남대학교 공업기술연구소 2001 工業技術硏究所論文集 Vol.29 No.1

This paper describes the development of sputtered tungsten electrode for discharge tube by making use of Sputtering method using plasma. The Xe discharge tube was designed and fabricated by using sputtered tungsten electrode, and the ICCD(Intensified Charged Coupled Device) and monochromator were used in order to observe the discharge image distribution and to measure the signal intensity. As a result, the emission light form the electrode was spreaded almost uniformly over a wide wavelength band, and the discharge image distribution and the signal intensity show quite a uniform distribution. The emission light from the Xe discharge tube was peaked at 488[nm] and 648[nm].

석면함유 슬레이트 지붕 물받이 퇴적물 중 석면 섬유 함유율

임지현,한솔민,김현석,신유민,박시은,허정윤,김민영,장봉기 순천향대학교 기초과학연구소 2022 순천향자연과학연구 논문집 Vol.28 No.1,2

This study attempted to determine the degree of asbestos release from the aging slate roof by comparing the asbestos content in the slate roof rain gutter with a colored steel plate (tin plate) over the slate roof. Four slate roof houses located in Haengmok-ri, Asan-si, Chungcheongnam-do, and one house constructed with a colored steel plate on the slate roof were selected to collect the sediment of the roof rain gutter. The asbestos fiber content was calculated by a point counting method using a polarization microscope after pretreatment with conversion treatment and hydrochloric acid treatment. The average asbestos content of the four slate roof rain gutter were 1.89%. However, asbestos was not detected in the Slate covering roof rain gutter, which were constructed on the slate roof. Asbestos fiber content was the highest at 2.89% in the slate roof rain gutter installed in 1976, followed by 2.44% in 1953. From the above results, it is necessary to minimize secondary damage as asbestos fibers released from slate roof houses to the surrounding atmosphere or leaked from slate roofs as rainwater may cause soil pollution and seriously affect residents' health. Although covering with colored steel plates (tin plates) has been shown to prevent the leakage of asbestos fibers to some extent, it is believed that a policy alternative to remove the slate roof as soon as possible is needed to solve the fundamental problem.

Analysis of <SUP>15</SUP>N-NH₄<SUP>+</SUP> stable isotope using ammonium diffusion technique

Si-yeong Park,Min-Seob Kim,Bo Ra Lim,Jaeseon Park,Eun-Jin Yoo,Sunkyoung Shin,Jeong-ki Yoon 한국분석과학회 2022 학술대회논문집 Vol.2022 No.5

Optical properties of DOM in Geum river watershed during monsoon period with storm event

Si Yeong Park,Min-Seob Kim,Bo Ra Lim,Pilyong Jeon,Seoyeon Hong,Da Rae Jeon,Sunhwa Hong,Eun mi Kim,Hyoung Seop Kim,Soohyung Lee,Yujeong Huh 한국분석과학회 2023 학술대회논문집 Vol.2023 No.11

LA-ICP/MS를 이용한 나무 나이테 중금속 농도 분석

박시영 ( Si-yeong Park ),김민섭 ( Min-seob Kim ),박재선 ( Jae-seon Park ),박현우 ( Hyun-woo Park ),김금희 ( Kum-hee Kim ),정현미 ( Hyen-mi Chung ),최종우 ( Jong-woo Choi ) 한국환경농학회 2018 한국환경농학회 학술발표논문집 Vol.2018 No.-

In order tn evaluate the precision and accuracy using LA-ICP/MS, thirty major and trace elements were analyzed with 3 USGS SRMs. And we investigated correlation between metal concentrations in five species tree-ring and ambient air heavy metal pollution history in ambient air. Total thirty major and trace elements in three USGS basalt glass reference materials, BIR-1G, BCR-2G and BHVO-2G were determined using LA-ICP/MS. Calibration was performed using NIST glass SRM 610, 612 and 614 in conjunction with internal standardization using Ca. Relative standard deviation (RSD) of determinations was below 10 % for most elements in all glasses under investigation. All the USGS SRMs showed negative correlations between RSD and concentrations for elements with logarithmic correlation coefficients ranging from -0.867 to -0.790. Furthermore, these values were similar results obtained in NIST glass SRM 610, 612, 614. In natural sample analysis, 7 heavy metals in 5 tree species (Platanus occidentalis, Salix koreensis, Chamaecyparis obtusa, Pinus densiflora, Ginkgo biloba) were determined using LA-ICP/MS, and calibration was performed using cellulose - matrix matched standards with 13C normalization. Four tree ring series showed that the Pb and Fe accumulation rates were higher between `92~`99 except for Ginkgo sp., but other elements (Cr, Mn, Cd, Zn, Sr) varied depending on species, which was caused by the physiological process of element uptake and radial mobility. Pb and Cd concentrations in tree ring of Pinus sp. have correlated with the ambient air heavy metal monitoring data which showed the correlation coefficients of 0.879 and 0.579, respectively. Pb and Cd concentrations in tree-ring of Salix sp. and Platanus sp. showed positive correlation with coefficients of 0.671 and 0.825, respectively. Therefore, our results suggested that Pb and Cd concentrations of Pinus sp. can be interpreted in terms of ambient air heavy metal pollution history.

TC/TN-IRMS를 이용한 수질시료 내 총 유기탄소 안정동위원소 분석법 소개

박시영 ( Si-yeong Park ),김민섭 ( Min-seob Kim ),임보라 ( Bo-ra Lim ),윤숙희 ( Suk-hee Yoon ),유은진 ( Hyen-mi Chung ),정현미 ( Jongwoo Choi ),최종우 한국환경농학회 2020 한국환경농학회 학술발표논문집 Vol.2020 No.-

The measurement of stable isotope ratios on total organic carbon (TOC) has long posed analytical problems and limited the use of this powerful tracer in biogeochemical studies in aquatic systems. Here, we provide a detailed description of a successful coupling of a custom-modified total organic carbon and nitrogen analyzer (Elementar, ISO TOC Cube) to an isotope-ratio mass spectrometer (Isoprime precision, CF-IRMS). The method is based on the wet oxidation of up to 20 mL aqueous sample in a closed reactor, produced CO₂ is carried over a water trap and purification column in a stream of He and introduced into the IRMS via a conventional open-split interface, enabling both quantification and δ¹³C analysis. Typical repeatability of δ¹³C analyses on TOC in this setup is in the order of 0.3‰ or better, comparable to that obtained in a more conventional elemental analyzer-IRMS setup. Typical overall system blank values for TOC analysis are in the order of 1 ㎍ C. Analytical conditions (reaction time, reagent concentrations) were optimized for samples, so that a single method can handle all types of environmental TOC samples. To evaluate a success of the method developed in this study, two international standards, IAEA-600 (δ¹³C = -27.7‰) and IAEA-CO-8 (δ¹³C = -5.7‰) were used. Measured their δ¹³C values were -27.7 ± 0.3‰ (n=100) for IAEA-600 and -5.7 ± 0.4‰ (n=100) for IAEA-CO-8, respectively, in excellent agreement with recommended values within the error. The method developed in this study will help researcher studying carbon cycle and tracing sources of TOC in the aquatic environment.

3개 실린더 주위의 비정상 유동 및 열전달 특성 연구

정영한(Yeong Han Jeong),김진영(Jin Yeong Kim),박정우(Jeong Woo Park),김시찬(Si Chan Kim),박태선(Tae Seon Park) 대한기계학회 2016 대한기계학회 춘추학술대회 Vol.2016 No.12

Unsteady flow and heat transfer characteristics around three cylinders are numerically analyzed. Under the condition of Re=100, the proximity effect of three circular cylinders are studied. As the distance between cylinders, the wall heat transfer rates are increased. The wall effects are disappeared for the distance of 1.5D. Also, the wall effect on the heat transfer rate is differently dependent on the cylinder angle.

증례 : 신장 ; 관상동맥 성형술 시행한 환자에서 Clopidogrel과 관련된 용혈성 요독증후군

박시형 ( Si Hyung Park ),박진한 ( Jin Han Park ),박상율 ( Sang Yool Park ),이은지 ( Eun Ji Lee ),전현수 ( Hyun Soo Jun ),김영훈 ( Yeong Hoon Kim ),김양욱 ( Yang Wook Kim ) 대한내과학회 2011 대한내과학회지 Vol.80 No.2S

Clopidogrel은 항혈소판제로 여러 임상영역에서 사용된다. 기존의 약제보다 부작용도 적어 안전하게 널리 쓸 수 있는 이 약제가 드물게는 이와 같은 심각한 합병증을 유발할 수 있다. 이에 우리는 이러한 합병증을 이해하고 주의 해야 한다. 저자들은 급성 관상동맥 증후군이 있었던 47세 남자 환자에서 이와 같은 합병증을 경험하였고, 약물 중단 및 치료적 혈장분리술 등으로 호전되었던 증례를 1예 경험하였기에 문헌고찰과 함께 보고하는 바이다. Thienopyridines are antiplatelet agents used in post-percutaneous coronary angioplasty patients and patients with acute coronary syndrome, stroke, and peripheral arterial disease. Ticlopidine has been shown to reduce the incidence of stent thrombosis, but it may also cause serious hematological side effects. Among the thienopyridines, clopidogrel is considered to be a safe alternative to ticlopidine because of its decreased incidence of hematological adverse effects. However, some hematological side effects can occur and may be fatal. In this case, a 47-year-old man complained of dyspnea and generalized edema. He had been taking clopidogrel after coronary angioplasty. His laboratory findings showed acute renal failure, microangiopathic hemolytic anemia, and thrombocytopenia, which were consistent with hemolytic uremic syndrome (HUS). After discontinuing clopidogrel and undergoing plasma exchange, he recovered fully. To our knowledge, this is the first reported case of clopidogrel-induced HUS in Korea. (Korean J Med 2011;80:S253-S257)