신장기능영상용 방사성의약품 Bz - MAG3 ( Benzoylmercaptoacetylglycylglycylglycine ) 의 키트화 및 체내분포

고창순(Chang Soon Koh),이명철(Myung Chul Lee),정준기(June Key Chung),이동수(Dong Soo Lee),김영주(Young Ju Kim),정재민(Jae Min Jeong),장영수(Young Soo Chang),조정혁(Jung Hyuk Cho) 대한핵의학회 1996 핵의학 분자영상 Vol.30 No.3

The MAG3 is a tubular excreting radiopharmaceutical for renal image. We synthe-sized benzoyl MAG3 (Bz-MAG3) and made a kit for labeling with Tc-99m. We checked the labeling effeciency of Tc-99m labeled MAG3 and biodistribution. Labeling efficiency was checked by TLC- SG (acetonitrile/H2O=2/1). After injecting of 1 mCi of Tc-99M-MAG3 to ICR-mice, Tmax(min), T1/2(min) were obtained in the renogram. Sequencial images (30sec, 2min, 5min, 10min, 15min, 20min) of TC-99m-MAG3 were compared with those of commercial Tc-99m-DTPA (Du Pont Merck Pharmaceutical Co.) kit. 1) The Rf value of synthesized Tc-99m-MAG3 was 0.78 and labeling efficiency was 97.5±1.9% (n=10). 2) The dynamic images of the Tc-99m-MAG3 were better than those of the Tc-99m-DTPA. 3) The Tmax(min.) and T1/2(min.) of Tc-99m-MAG3 (n=10) were 1.5±0.5 (left), 1.4±0.4(right), and 4.3±1.4 (left), 4.8±2.0 (right), respectivel. The Tmax(min.) and T1/2(min.) of Tc-99m-DTPA (n=7) were 2.7±1.6 (left), 2.7±1.6 right), and 3.8±1.7 (left), 4.5±2.7 (right), respectively. The quaility of image and labeling efficiency of the synthesized Bz-MAG3 kit were excellent, that it was supposed to be used in routine clinical work.

Dose-linear pharmacokinetics of oleanolic acid after intravenous and oral administration in rats

Jeong, Dong Won,Kim, Young Hoon,Kim, Hui Hyun,Ji, Hye Young,Yoo, Sun Dong,Choi, Won Rack,Lee, Soo Min,Han, Chang-Kyun,Lee, Hye Suk WILEY PUBLISHERS 2007 BIOPHARMACEUTICS AND DRUG DISPOSITION Vol.28 No.2

<P>The pharmacokinetics of oleanolic acid was evaluated in vitro and in vivo. From Caco-2 cell permeation studies, oleanolic acid was a low permeability compound with no directional effects, suggesting a low in vivo absorption mediated by a passive diffusion. Oleanolic acid was metabolically unstable following incubation with rat liver microsomes in the presence of NADPH. After intravenous injection at doses of 0.5, 1 and 2 mg/kg doses, oleanolic acid showed dose-linear pharmacokinetics as evidenced by unaltered CL (28.6–33.0 ml/min/kg), V<SUB>ss</SUB> (437–583 ml/kg), dose-normalized AUC (16.0–17.9 µg min/ml based on 1 mg/kg) and t<SUB>1/2</SUB> (41.9–52.7 min). Following oral administration of oleanolic acid at doses of 10, 25 and 50 mg/kg, T<SUB>max</SUB>, t<SUB>1/2</SUB>, dose-normalized C<SUB>max</SUB> (66–74 ng/ml based on 25 mg/kg) and dose-normalized AUC (5.4–5.9 µg min/ml based on 25 mg/kg) were comparable between 25 and 50 mg/kg dose, but the plasma concentrations at 10 mg/kg dose were not measurable as they were below the limit of quantitation (2 ng/ml). The absolute oral bioavailability was 0.7% for oral doses of 25 and 50 mg/kg. The extent of urinary excretion was minimal for both i.v. and oral doses. The very low oral bioavailability of oleanolic acid could be due to a poor absorption and extensive metabolic clearance. Copyright © 2007 John Wiley & Sons, Ltd.</P>

Crosslinked anion exchange membranes with primary diamine-based crosslinkers for vanadium redox flow battery application

Cha, Min Suc,Jeong, Hwan Yeop,Shin, Hee Young,Hong, Soo Hyun,Kim, Tae-Ho,Oh, Seong-Geun,Lee, Jang Yong,Hong, Young Taik Elsevier 2017 Journal of Power Sources Vol.363 No.-

<P><B>Abstract</B></P> <P>A series of polysulfone-based crosslinked anion exchange membranes (AEMs) with primary diamine-based crosslinkers has been prepared via simple a crosslinking process as low-cost and durable membranes for vanadium redox flow batteries (VRFBs). Chloromethylated polysulfone is used as a precursor polymer for crosslinked AEMs (CAPSU-x) with different degrees of crosslinking. Among the developed AEMs, CAPSU-2.5 shows outstanding dimensional stability and anion (Cl<SUP>−</SUP>, SO<SUB>4</SUB> <SUP>2−</SUP>, and OH<SUP>−</SUP>) conductivity. Moreover, CAPSU-2.5 exhibits much lower vanadium ion permeability (2.72 × 10<SUP>−8</SUP> cm<SUP>2</SUP> min<SUP>−1</SUP>) than Nafion 115 (2.88 × 10<SUP>−6</SUP> cm<SUP>2</SUP> min<SUP>−1</SUP>), which results in an excellent coulombic efficiency of 100%. The chemical and operational stabilities of the membranes have been investigated via ex situ soaking tests in 0.1 M VO<SUB>2</SUB> <SUP>+</SUP> solution and in situ operation tests for 100 cycles, respectively. The excellent chemical, physical, and electrochemical properties of the CAPSU-2.5 membrane make it suitable for use in VRFBs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The crosslinking between polymer chains are constructed by 4,4′-diaminobenzophenone. </LI> <LI> The crosslinked AEMs were fabricated by commercial polysulfone (PSU; Udel<SUP>®</SUP> P-3500). </LI> <LI> The crosslinked AEM based on PSU indicated good chemical and dimensional stability. </LI> <LI> The performance of crosslinked AEM showed high EE (86%) with high capacity retention. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

The Effect of pH and Various Cations on the GTP Hydrolysis of Rice Heterotrimeric G-protein α Subunit Expressed in Escherichia Coli

( Hak Soo Seo ),( Jin Yong Jeong ),( Min Yeop Nahm ),( Sam Woong Kim ),( Sang Yeol Lee ),( Jeong Dong Bahk ) 생화학분자생물학회 2003 BMB Reports Vol.36 No.2

Previously, we reported the biochemical properties of RGAl that is expressed in Escherichia coli(Seo et al., 1997). The activities of RGAl that hydrolyzes and binds guanine nucleotide were dependent on the MgCl_(2) concentration. The steady state rate constant (k_(cat)) for GTP hydrolysis of RGAl at 2 mM MgCl_(2) was 0.0075±0.0001 min^(-1). Here, we examined the effects of pH and cations on the GTPase activity. The optimum pH at 2mM MgCl_(2) was approximately 6.0; whereas, the pH at 2 mM NH_(4)Cl was approximately 4.0. The result from the cation dependence on the GTPase (guanosine 5`-triphosphatase) activity of RGAl under the same condition showed that the GTP hydrolysis rate (k_(cat)=0.0353 min^(-1)) under the condition of 2mM NH_(4)Cl at pH 4.0 was the highest. It corresponded to about 3.24-fold of the k_(cat) value of 0.0109 min^(-1) in the presence of 2mM MgCl_(2) at pH 6.0.

초기 자궁내막암 환자에서의 복강경유도하 질식 자궁절제술과 복식 자궁절제술에 대한 비교 연구

김민규 ( Min Kyu Kim ),최동석 ( Dong Seok Choi ),김우영 ( Woo Young Kim ),최철훈 ( Chel Hun Choi ),김태중 ( Tae Joong Kim ),이정원 ( Jeong Won Lee ),김병기 ( Byoung Gie Kim ),이제호 ( Je Ho Lee ),배덕수 ( Duk Soo Bae ) 대한산부인과학회 2006 Obstetrics & Gynecology Science Vol.49 No.12

Objective: To compare laparoscopic surgery with conventional abdominal surgery in patients with early stage endometrial cancer. Methods: A retrospective review of 63 patients with early stage endometrial cancer managed between March 2003 and May 2005. Two groups were defined whether they had been treated by laparoscopy (case group: n=26) or by laparotomy (control group: n=37). We compared age, body mass index (BMI), hemoglobin change, operation time, number of pelvic lymph nodes, hospital stay, case with adjuvant treatment and recurrence between two groups. Results: There was no statistical difference in characteristics (age, BMI, nulliparity, previous abdominal surgery, FIGO stage, histologic grade). between case and control group. In addition, there was no statistical difference in operation data and outcomes between two groups. Hemoglobin changes were 1.1 g/dL (case group) vs 1.7 g/dL (control group) (p=0.072). Operation time was 131 min vs. 115 min. The numbers of lymph nodes obtained were 8.7 vs 7.7 (right) 9.2 vs. 7.6 (left). Hospital stays were 8.4 vs. 9.2 days. Adjuvant treatment cases were 7 vs. 15. Recurrent case was one in each group. Two patients initially evaluated by laparoscopy were converted to laparotomy due to bleeding and adhesion. Conclusion: Laparoscopic surgery for treatment of early endometrial cancer is a safe and effective alternative to laparotomy . However, long-term survival and risk of recurrence have yet to be determined.

허웅의 ‘훈민정음’에 대한 인식과 영향관계 : 19세기 말∼20세기 전반기, ‘국어학’ 학술네트워크 고찰을 위하여

서민정(Seo, Min-jeong),최규수(Choi, Kyu-soo) 부산대학교 인문학연구소 2020 코기토 Vol.- No.90

‘훈민정음’은 일제강점기 이후 일본 제국에 저항할 수 있는, 민족적 자부심이 담긴 대표적인 유산으로 여겨왔다. 그리고 그러한 인식은 19세기 말∼20세기 초의 지식인, 특히 국어학자들의 훈민정음에 대한 연구성과와 직접 연결되어 있다. 특히 ‘허웅’은 20세기 전반기의 국어학 연구나 언어에 대한 인식 등을 현재까지 잇는 중간 역할을 하였다는 측면에서 ‘허웅’의 훈민정음 인식에 대해 주목할 필요가 있다. 이 연구는 앞선 연구에서 주로 다루어 온 주시경, 권덕규, 김윤경, 최현배, 홍기문 등의 연구 성과를 바탕으로 허웅의 ‘훈민정음’에 대한 인식이나 연구에 대해 고찰하고자 한다. 그것은 허웅(1974가, 나)에서 볼 수 있듯이 그의 말과 글의 관계나, 한글전용과 같은 언어 정책에 대한 입장 등은 대부분 ‘훈민정음’ 특히 ‘어제서문’의 해석과 관련하여 설명되고 있어서 그의 ‘훈민정음’에 대한 인식을 검토할 필요성이 있기 때문이다. 한편 허웅은 학문에 대한 태도는 실증적이고 엄정하다는 평가를 받고 있고 스스로도 그러한 관점에서 연구하고자 하였다. 그러나 훈민정음 서문의 해석에서는 앞선연구의 전통을 그대로 이어받아 실증적이고 비판적인 태도를 찾아보기 힘들었다. 이 연구에서는 그러한 허웅의 관점이 허웅의 학문적 태도에서 출발했다기보다는 훈민정음에 대한 우리의 인식이 그만큼 맹목적인 부분이 있었기 때문이었음을 강조하고자 하였다. 허웅의 훈민정음에 대한 인식은 『한글과 민족 문화』(1974ㄱ)에 선명히 나타나 있는데, 이외에도 『우리말과 글의 내일을 위하여』(1974), 『우리말과 글에 쏟아진 사랑』(1979), 「세종의 언어 정책과 국어 순화 정신」 (1980), 『이삭을 줍는 마음으로』(1981) 등에서도 그의 훈민정음에 대한 인식을 엿볼 수 있다. 이 연구에서는 위의 자료를 바탕으로 허웅의 훈민정음에 대한 인식을 고찰하고자 한다. Since the flowering period, ‘Hunminjeongeum’ has been understood as a representative heritage showing the pride of the people who oppose the Western modernity or resist the Japanese empire. In this process, it was natural that the research related to the Hunminjeongeum of intellectuals, especially Korean scholars, became the basis. So far, in the previous study, discussions about Hunminjeongeum such as Ju Si Kyung, Kwon Duk Kyu, Kim Yoon Kyung, Choi Hyun Bae, and Hong Gi Moon have been discussed to some extent. However, there is a need for this research in that the review of the ‘Hunminjeongeum’ by Mr. Huh has not been done yet. As we can see in Huh Woong(1974, I) in particular, the relationship between his words and writings, and the position of language policy, such as Hangul, are mostly explained in relation to the contents of ‘Hunminjeongeum’, especially ‘Preface’, which makes it more necessary to examine his perception of ‘Hunminjeongeum’. On the other hand, as discussed in Seo Min-jeong(2011), “Hunminjeongeum” and “Preface” were translated into the language of the 15th century (say) and translated into the language of the 20th century in the first half of the 20th century. The material that reveals Huh Woong’s awareness of Hunminjeongeum is “Hangul and National Culture”(1974). Other than that, for the sake of Korean and Writing tomorrow (1974), Korean and Writing Love(1979), Sejong’s Language Policy and Korean Refinement Spirit(1980), Isaac’s Mind, etc.(1981), and the Korean and Korean Cultures(1974, Sejong University, and the Korean People’s Association(1974), The Korean language policy is also discussed, and the teacher’s recognition of the Hunminjeongeum can be said to be followed by the Korean language policy. Based on the above data, I would like to examine the perception of Hunminjeongeum by Mr. Huh. In this study, we found that the attitude of Huh Hung"s scholarship showed a different pattern in the interpretation of the preface of Hunminjeongeum. And it was examined that such aspect did not overcome the limitations of the situation of the times rather than the problem of attitude of Heung. Based on these considerations, we should recognize that it is time to reinterpret and evaluate Hunminjeongeum.

The Effect of pH and Various Cations on the GTP Hydrolysis of Rice Heterotrimeric G-protein α Subunit Expressed in Escherichia Coli

Seo, Hak-Soo,Jeong, Jin-Yong,Nahm, Min-Yeop,Kim, Sam-Woong,Lee, Sang-Yeol,Bahk, Jeong-Dong Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.2

Previously, we reported the biochemical properties of RGA1 that is expressed in Escherichia coli (Seo et al., 1997). The activities of RGA1 that hydrolyzes and binds guanine nucleotide were dependent on the $MgCl_2$ concentration. The steady state rate constant ($k_{cat}$) for GTP hydrolysis of RGA1 at 2 mM $MgCl_2$ was $0.0075{\pm}0.0001\;min^{-1}$. Here, we examined the effects of pH and cations on the GTPase activity. The optimum pH at 2 mM $MgCl_2$ was approximately 6.0; whereas, the pH at 2 mM $NH_4Cl$ was approximately 4.0. The result from the cation dependence on the GTPase (guanosine 5'-triphosphatase) activity of RGA1 under the same condition showed that the GTP hydrolysis rate ($k_{cat}=0.0353\;min^{-1}$) under the condition of 2mM $NH_4Cl$ at pH 4.0 was the highest. It corresponded to about 3.24-fold of the $k_{cat}$ value of $0.0109\;min^{-1}$ in the presence of 2 mM $MgCl_2$ at pH 6.0.

담체가 Re-188-Hydroxyethylidene Diphosphonate의 표지와 생체내분포에 미치는 영향

장영수(Young Soo Chang),정재민(Jae Min Jeong),김보광(Bo Kwang Kim),조정혁(Jung Hyuk Cho),이동수(Dong Soo Lee),정준기(June Key Chung),이승진(Seung Jin Lee),김인걸(In Kul Kim),이상은(Sang Eun Lee),이명철(Myung Chul Lee) 대한핵의학회 2000 핵의학 분자영상 Vol.34 No.4

Purpose: Re-188-Hydroxyethylidene diphosphonate (HEDP) is a new cost-effective agent for systemic radioisotope therapy of metastatic bone pain. We investigated the influence of carrier for labeling and biodistribution of Re-188-HEDP using HEDP kit with or without carrier (KReO4). Materials and Methods: The kits (HEDP 15 mg, gentisic acid 4 mg and SnCl2.2H2O 4.5 mg) with or without carrier (KReO4 0.1 mg) were labeled with Re-188 solution, made available from an in-house generator by boiling for 15 min. We compared the labeling efficiency and stability of carrier-added and carrier-free preparations of Re-188-HEDP. Biodistribution and imaging studies of each preparation were performed in ICR mice (1.85∼3.7 MBq/0.1 ml) and SD rats (74.1∼85.2 MBq/0.5 ml). Results: The carrier-added preparation showed high labeling efficiency (95% at pH 5) and high stability in serum (88%, 3 hr). However, the carrier-free preparation showed low labeling efficiency (59% at pH 5) and low stability (43%, 3 hr). The carrier-added preparation showed high uptake in bone and low uptake in stomach and kidneys. However, the carrier-free preparation showed lower uptake in bone and higher uptake in both stomach and kidneys, which is supposed to be due to released perrhenate. The carrier-added preparation also showed better images with higher skeletal accumulation, lower uptake in other organs and lower soft tissue uptake than the carrier-free preparation. Conclusion: The results of these studies clearly demonstrate that addition of carrier perrhenate is required for high labeling efficiency, stability, bone uptake and good image quality of Re-188-HEDP. (Korean J Nucl Med 2000;34:344-52)

분변토를 이용한 VOCs 흡착에 관한 기초연구

김재홍,손희정,김장호,김수생,서정민 동아대학교 환경문제연구소 1997 硏究報告 Vol.20 No.2

This study was carried out two point view that reuse of sludge and adsorption of benzene, toluene and o-xylene of VOCs in cast, carbonized cast and activated carbon. The cation exchange capacity of cast and carbonized cast were 59.2, 112 meq/l00g, respectively. The specific surface were 560, 800 ㎡/g, respectively. The average removal rates of benzene by 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 15.0, 41.2, 88.2, 99.4% in 60min retention time. The average removal rates of toluene by 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 12.5, 34.2, 88.2, 99.5% in 60min retention time. The average removal rates of o-xylene 50g cast of 50% hydrous cast, anhydrous cast, carbonized cast, activated carbon were 8.8, 28.5, 84.8, 98.1% in 60min retention time. The adsorption efficiency of test absorbent was in order of benzene > toluene > o-xylene.

친환경 계면활성제 Potassium Cocoyl Glycinate (PCG)가 알칼리 전처리된 백합나무의 효소 당화에 미치는 영향

민병철(Byeong-Cheol Min),정수빈(Subin Jeong),송우용(Woo-Yong Song),성현아(Hyun-A Seong),신수정(Soo-Jeong Shin) 한국펄프·종이공학회 2018 펄프.종이기술 Vol.50 No.1

Potassium cocoyl glycinate (PCG) as a green surfactant was used to evaluate the possibility to substitute petrochemical surfactants for productivity improvement of enzymatic hydrolysis. Yellow poplar (Liriodendron tulipifera L. ) was subjected to NaOH (0.1%, 1.0% and 10.0%) pretreatment at 121°C for 60 min. Pretreated substrate (5.0% w/v) was hydrolyzed by enzyme under the variety PCG concentrations up to 0.3%. The optimum dose of PCG was around 0.03% at which it resulted in the highest total sugar production for all pretreatment conditions. The total sugar production of pretreated yellow poplar with 0.1%, 1.0% and 10.0% of NaOH increased from 2.3, 20.1, 20.8 g/L to 7.7, 25.7, 31.8 g/L under 0.03% of PCG concentration at 72 h hydrolysis. The effect of PCG to improve enzymatic hydrolysis was significantly promising as compared to other researches applying surfactants for hydrolysis. In conclusion, application of PCG was a promising alternative to replace petrochemical surfactants in enzymatic hydrolysis processes due to the high sugar production and positive influence on the ecosystem.