http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 황성민(Hwang seong min) (검색결과 21 건)
- 무료
- 기관 내 무료
- 유료
-
황성민,서성화,박인명,최경화,김도만,차재호,Hwang, Sung-Min,Seo, Seong-Hwa,Park, In-Myoung,Choi, Kyoung-Hwa,Kim, Do-Man,Cha, Jae-Ho The Korean Society for Microbiology and Biotechnol 2012 한국미생물·생명공학회지 Vol.40 No.2
Glycosynthase는 친핵성 아미노산을 비친핵성 아미노산으로 치환하여 당전이 산물의 가수분해를 막아서 당전이 효율을 증가시킬 수 있다. 이전 연구에서 본 실험실은 열에 안정하고 산에 강한 Thermoplasma acidophilum 유래의 ${alpha}$-glucosidase (AglA)가 당전이 활성이 있음을 입증하였으나 시간이 지남에 따라 당전이 산물이 가수분해 되었다. 이러한 AglA의 당전이 효율을 개선하기 위하여 친핵성 아미노산인 아스파라긴산을 글리신으로 치환하였다. 이 치환된 glycosynthase는 니켈 친화력 크로마토그래피를 통하여 정제되었으며, 정제된 돌연변이 단백질의 배당체를 합성하는 능력이 말토오스를 공여체로 그리고 p-nitrophenyl-${alpha}$-D-glucopyranoside($pNP{\alpha}G$)를 수용체로, 그리고 $pNP{\alpha}G$가 당공여체 및 수용체로 이용될 수 있는지 검사하였다. Glycosynthase를 이용한 당전이 산물의 수율은 약 42.5%를 보였으며 시간이 지남에 따라서 가수분해되지 않았다. 박막 크로마토그래피법을 이용한 반응산물의 분석은 수용체의 높은 농도에서 기존의 효소보다 많은 양의 배당체를 합성할 수 있음을 보여주었고, 특히 중성보다 낮은 pH 영역에서 가장 높은 활성을 보여줌을 확인하였다. 이러한 결과는 glycosynthase가 산업적으로 배당체를 합성하는데 유용성이 크다는 것을 나타낸다. Glycosynthase is an active site nucleophile mutant enzyme, prepared from glycosidase, which is capable of synthesizing oligosaccharide derivatives without the hydrolysis of the product. Thermoacidophilic ${\alpha}$-glucosidase of Thermoplasma acidophilum (AglA) exhibits a transglycosylating activity yielding various glycosides. AglA was converted to glycosynthase by the substitution of the catalytic nucleophile Asp-408 residue into non-nucleophile glycine in order to increase its ability to synthesize various glycosides by transglycosylation. The glycosynthase mutant was purified by Ni-NTA chromatography and its glycoside-synthesizing activity was measured by using an external nucleophile, sodium formate buffer, providing maltose as a donor and p-nitrophenyl-${\alpha}$-D-glucopyranoside ($pNP{\alpha}G$) as an acceptor, respectively. In addition, $pNP{\alpha}G$ was examined for its feasibility to act as both a donor and an acceptor, and products were compared with those of the wildtype enzyme. The mutant enzyme was found to catalyze the formation of a specific product from $pNP{\alpha}G$ with a yield of 42.5% without further hydrolysis, while the wild-type enzyme produced two $pNP{\alpha}G$ products at low yields. The results demonstrate the possibility of satisfactory yields for the reactions in the presence of small amounts of acceptor, and demonstrate that the high activity of the mutant, at pHs below neutrality, was applicable in the transfer of glucose from the natural donor.
-
황성민,노광명,정명준,허민,정하풍,서정원,박찬광,고요환,이대훈,Hwang, Seong-Min,Rho, Kwang-Myoung,Chung, Myung-Jun,Huh, Min,Jeong, Ha-Poong,Suh, Jeong-Won,Park, Chan-Kwang,Koh, Yo-Hwan,Lee, Dai-Hoon 대한전자공학회 1995 전자공학회논문지-A Vol.32 No.6
We proposed SAW (Self-Algined Selectively Grown W-Gate) MOSFET structure, and strudied electrical characteristics of the fabricated SAW MOSFETs. The threshold volgate of 0.21${\mu}$m SAW NMOSFET was 0.18 V and that of 0.24 ${\mu}$m SAW PMOSFET was -0.16 V. The subthreshold slope was 74 mV/decade for NMOSFET and 82 mV/decade for PMOSFET. The maximum transconductance of NMOSFET and PMOSFET, at V$_{GS}$=2.5 V and V$_{DS}$=1.5 V, were260 mS/mm and 122 mS/mm. The measured saturation drain current at V$_{GS}$=V$_{DS}$ =2.5 V was 0.574 mA/${\mu}$m for NMOSFET and -0.228 mA/${\mu}$m for PMOSFET. The gate resistance of SAW MOSFET was about m$\Omega$cm and the n+-p junction capacitance of SAW MOSFET was about 10% lowas than that of the conventional MOSFET's.
-
-
-
자기 주도적 학습이 강화된 조별 발표 물리 수업이 마이스터고 학생의 과학 관련 태도와 의사소통능력에 미치는 영향
황성민(Seong-Min Hwang), 최혁준(Hyukjoon Choi) 한국교원대학교 뇌·AI기반교육연구소 2020 Brain, Digital, & Learning Vol.10 No.4
The purpose of this study was to investigate the effects of group-led presentations with enhanced self-directed learning on science-related attitudes and communication skills of Meister high school students. Pre- and post-tests on science-related attitudes and communication skills were conducted, and surveys were conducted to determine class satisfaction. The conclusion of this study was as follows; First, the application of group presentations, which strengthened self-directed learning, positively changed students’ science-related attitudes. Second, the application of group presentations, which strengthened self-directed learning, did not give a positive result to the students communication ability.
-
-
복귀돌연변이시험 및 단회투여독성시험을 통한 맥문동 물 추출물의 안전성 평가
황성민(Seong Min Hwang),조성영(Sung Young Cho),김수영(Su Yeong Kim),노종현(Jong Hyun Nho) 대한한의학회 2025 대한한의학회지 Vol.46 No.3
Objectives: Liriope platyphylla has been traditionally used in Korean medicine for relieving cough, reducing lung heat, and nourishing the yin. However, scientific data regarding the safety of its water extract reamins insufficient. This study was conducted to evaluate the genotoxicity and acute oral toxicity of the Liriope platyphylla water extract through a bacterial reverse mutation test and a single-dose oral toxicity study in rats.<BR/>Methods: This study evaluated the genotoxic and acute oral toxicity of the Liriope platyphylla water extract. A bacterial reverse mutation assay (Ames test) was conducted using Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, as well as Escherichia coli WP2 uvrA, both with and without metabolic activation (S9 mix). Additionally, a single-dose oral toxicity study was performed in Sprague-Dawley rats according to OECD Test Guideline 423.<BR/>Results: No increase in revertant colonies or dose-dependent mutagenicity was observed in any strain, regardless of the presence of S9 mix. In the acute oral toxicity test, no mortality, clinical signs, or pathological changes were found at doses up to 2,000 mg/kg.<BR/>Conclusions: These results suggest that the Liriope platyphylla water extract is non-mutagenic and does not exhibit acute oral toxicity after single-dose administration, supporting its potential safety as a herbal resource for further development
-
-
Thermoplasma acidophilum 유래 α-glucosidase로 부터 생산된 glycosynthase 돌연변이 단백질의 개선된 당전이 효율
황성민 ( Sung Min Hwang ),서성화 ( Seong Hwa Seo ),박인명 ( In Myoung Park ),최경화 ( Kyoung Hwa Choi ),김도만 ( Do Man Kim ),차재호 ( Jae Ho Cha ) 한국미생물생명공학회 2012 한국미생물·생명공학회지 Vol.40 No.2
Glycosynthase is an active site nucleophile mutant enzyme, prepared from glycosidase, which is capable of synthesizing oligosaccharide derivatives without the hydrolysis of the product. Thermoacidophilic α-glucosidase of Thermoplasma acidophilum (AglA) exhibits a transglycosylating activity yielding various glycosides. AglA was converted to glycosynthase by the substitution of the catalytic nucleophile Asp-408 residue into non-nucleophile glycine in order to increase its ability to synthesize various glycosides by transglycosylation. The glycosynthase mutant was purified by Ni-NTA chromatography and its glycoside-synthesizing activity was measured by using an external nucleophile, sodium formate buffer, providing maltose as a donor and pnitrophenyl- α-D-glucopyranoside (pNPαG) as an acceptor, respectively. In addition, pNPαG was examined for its feasibility to act as both a donor and an acceptor, and products were compared with those of the wildtype enzyme. The mutant enzyme was found to catalyze the formation of a specific product from pNPαG with a yield of 42.5% without further hydrolysis, while the wild-type enzyme produced two pNPαG products at low yields. The results demonstrate the possibility of satisfactory yields for the reactions in the presence of small amounts of acceptor, and demonstrate that the high activity of the mutant, at pHs below neutrality, was applicable in the transfer of glucose from the natural donor.
-
에탄올 수용액의 Pervaporation 에서의 투과거동
배성렬 ( Seong Youl Bae ),이한선 ( Han Sun Lee ),황성민 ( Seong Min Hwang ),김희택 ( Hee Taik Kim ),히데히로구마자와 ( Hidehiro Kumazawa ) 한국공업화학회 1994 공업화학 Vol.5 No.1
In the process of pervaporation separation for aqueous ethanol solution through cellulose tri-acetate(CTA) membrane, the modelling on the solution-diffusion permeation mechanism was built up on the basis of sorption and permeation experimental results. Also its function type and parameter were examined. The composition of sorption equilibrium in three component system(Ethanol/Water/CTA) were compared with the calculated value by Flory-Huggins` equation using the pure component sorption data. In order to apply the thermodynamic equilibrium relationship between the membrane free composition in the membrane and the equilibrium composition in the liquid phase, the apparent activity coefficients in the membrane were correlated by Wilson and Van Laar type equations. Due to the nonideal behaviour in this system, however, the results were not satisfied. Diffusion equations were expressed with the concentration gradient considering permeate alone, and a concentration-dependent diffusion coefficient which includes a parameter was used. And this model was fitted with the measured permeation rates. If the permeation rate and the amount of sorption of one component were much larger than those of the other, the bulk flow term could not be negligible. The flux and selectivity were increased with increasing temperature, and with decreasing downstream pressure.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
