서프레셔를 적용한 액체금속이온원의 에너지 퍼짐 연구

민부기,오현주,조병성,강승언,최은하 한국진공학회 2011 한국진공학회 학술발표회초록집 Vol.40 No.0

UASB의 반응온도가 축산폐수 처리에 미치는 영향

민부기,이창현,최승렬,윤경진,권오훈,김재용 한국공업화학회 2012 한국공업화학회 연구논문 초록집 Vol.2012 No.-

운동에 의한 과활성산소종의 형성이 정신건강에 미치는 영향에 관한 연구

민부기 국제선무학회 2006 禪武學術論集 Vol.- No.16

PCM의 마이크로캡슐화 및 그 특성분석

민부기,권오훈,김진혁,윤경진,이창현,김재용 한국공업화학회 2011 한국공업화학회 연구논문 초록집 Vol.2011 No.-

뇌혈관 조영술에서 Neurologic complication과 Foreign-material에 대한 연구

민부기,안승원,김종만,대창민,고중석,민관홍,김성룡 대한인터벤션영상기술학회 2012 대한인터벤션영상기술학회지 Vol.15 No.1

목적: 뇌혈관 조영술 (Transfemoral Cerebral Angiogrphy: TFCA)의 부작용 (complication)에 대해 알아보고, 뇌혈관 조영술에 사용되는 시술재료에서 발생될 수 있는 Foreign material에 의한 Micro-embolism Risk에 대해 알아보고자 한다. 대상 및 방법: 뇌혈관 조영술에 사용되는 시술재료 중 공통적으로 쓰이는 조영제 자동주입기 연결선과 진단용 카테터를 대상으로 실험하였다. 실험은 뇌혈관 조영술과 동일한 조건으로 재현하였고, 새 제품과 재사용 제품을 비교하여 재사용 제품의 안전성을 분석하였다. 검사는 Filtration Method와 Cut and Microscope Method로 관찰, 분석하였다. 이때 측정 편차에 따른 오차를 줄이기 위해 방사선사 3명, 연구원 2명이 각각 동일한 방법으로 실험에 참여하였다. 결과: 실험결과 80개의 시술재료에서 8건의 Foreign material이 관찰되었다. Filtration Method에서는 Foreign material이 관찰되지 않았으나, Cut and Microscope Method에서 Foreign material이 발견되었다. 새 제품과 재사용 제품에서 모두 Foreign material이 발견되었고, 크기는 두 제품 공통적으로 100μm 미만이었다. 측정결과 발견된 Foreign material의 크기는 조영제 자동주입기 연결선에서 33.07~63.95μm, 진단용 카테터에서 24.84~52.71μm였다. 결론: 실험결과 Foreign material이 발견 되었지만 실제 시술과정을 재현한 Filtration method에서는 검출되지 않았다. Cut and Microscope method에서 발견된 7개의 입자 크기가 모두 100μm 미만으로 현미경으로 확인이 가능한 작은 입자였다. 그러나 Joseph H. Rapp는 60-100μm 미만의 입자에서도 뇌경색 (Infarction)이 발생할 수 있다.'고 발표 하였다.1 따라서 실험에서 발견된 Foreign material의 크기가 작고, 실제 조영제 주입시에 검출되지 않았더라도 complication 발생 가능성을 염두에 두고 시술에 임하여야 할 것이다.

임신과 수유기간동안 약물사용

閔富基 대한의사협회 1998 대한의사협회지 Vol.41 No.5

친환경적 그린 LED용 Power Inductor 개발

민부기,김진혁,권오훈,윤경진,이창현,김재용 한국공업화학회 2011 한국공업화학회 연구논문 초록집 Vol.2011 No.1

체외배양에서 인간 난포액이 생쥐의 배 발달에 미치는 영향

민부기,최기욱,김기석,이희섭,홍기연,이봉주,이선영,박승택,Min, Bu-Kie,Choi, Ki-Wook,Kim, Kie-Suk,Lee, Hee-Sub,Hong, Ki-Yeon,Lee, Bong-Ju,Lee, Sun-Young,Park, Seung-Teak 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

The follicular fluid (FF) of ovary contains various biological active products which affected on the growth of follicles and the fertilization of oocyte in physiological reproductive process of mammals. This study was designed to determine the effects of human FF on fertilization of oocyte and embryonal development in vitro culture. The FF was prepared as clear without blood contamination by needle aspiration from mature follicles of human at the time of oocytes retrieval for in vitro fertilization (IVF). As the medium for culture in vitro of embryonal cells, human tubal fluid (HTF) supplemented with follicular fluids at concentrations of 10%, 40% and pure FF were used. These effects were compared to control group of cultured embryos in HTF supplemented with 0.4% BSA (bovine serum albumin). For IVF, 64 eggs in control group, 67 eggs in 10% FF, 57 eggs in 40% FF and 64 eggs in pure FF were respectively allocated. And the rates of fertilization were almost similar in all groups as resulting 82.81% in control, 85.07% in 10% FF, 87.71% in 40% FF and 81.25% in pure FF. On the examination for embryonal cleavage from fertilized eggs, the rates of developing to 4 cell stage was similar in all groups, as results 98.11% in control, 98.27% in 10% FF and 98% in 40% FF but 78.84% in pure FF. And the rates of developing to 8-16 cell stage were significantly reduced as 44% in 40% FF and 44.23% in pure FF (p<0.05) compare to 71.69% in control media. As likewise, the rates of developing to morular stage were also significantly reduced to 36% (p<0.05) and 21.15% (p<0.01) respectively in 40% FF and pure FF. And the rates to blastocystic stage of embryo was lowest as 7.69% in pure FF (Table 1). The quality of embryonal cells on cleavage to the 8-16 cell stage was poorer, higher concentrations of FF. The rates of grade 1 in pure FF, as 23.07%, was lowest compare to those of other groups, in which the rates of grade 1 in control, 10% FF and 40% FF were 58.49%, 47.36% and 34% respectively. And on the contrary, the rate of grade 4 in pure FF was highest as 23.07%, while those were 5.66% in control, 8.77% in 10% FF and 20% in 40% FF (Table 2). On the viability of embryos, the rate of embryonal cell death was more rise, at the higher concentrations as well as longer exposure in the follicular fluid. At 48 hours after in vitro culture of embryos, the rate of survival embryos in pure FF was markedly lowered as 44.23%, compare to that of control (p<0.05). But there was not significant difference between the rates of survival embryos in each group beside the pure FF, which the rates were 77.35% in control, 70.17% in 10% FF and 60% in 40% FF respectively. And at 72 hours after in vitro culture, the rates of survival embryos were also significantly dropped to 21.15% in pure and 36% in 40% at concentration of FF compare to 62.26% in control (p<0.05, p<0.01). Finally, the rate of embryonal death at 96 hours after in vitro culture was highest as 82.69% in pure FF among all groups which those were 35.84 in control, 56.14% in 10% FF and 64% in 40% FF respectively (Fig. 1, 2, 3). In conclusion, this study suggests that the FF has no effects, in particular, to the in vitro fertilization of oocytes but exerted a bad effect to the cleavage, quality and viability of the embryonal cells during in vitro culture. However, the FF is harmful on embryonal development at conditions in higher concentration and especially on the embryos after $8{\sim}16$ cell stage.

第十四回 法의날 紀念辭

閔復基 한국법학원 1977 한국법학원보 Vol.- No.59

인간 난관세포와의 체외 공동배양과정에서 혈소판 활성요소가 생쥐배의 발달에 미치는영향

민부기,김기석,이희섭,홍기연,김흥곤,신무철,이찬근,최은하,Min, Bu-Kie,Kim, Kie-Seok,Lee, Hee-Sup,Hong, Kie-Youn,Kim, Heung-Gon,Shin, Mu-Cheol,Lee, Chan-Kun,Choi, Eun-Ha 대한생식의학회 1996 Clinical and Experimental Reproductive Medicine Vol.23 No.1

There are a number of problems during the process of culture in vitro on fertilization and embryo development compared to those on in vivo counterparts. And the platelet activating factor (PAF), which is found not only in mammalian spermatozoa but also preembryos, is implicated on reproductive process. To improve the environment of culture on in vitro fertilization and embryo development, coculture using salpingeal epithelial cells has been considered to accept the better result on pregnancy rate. This study was designed to determine if two different culture systems, coculture alone and PAF treated coculture, are positive or negative influence on process of in vitro fertilization and embryo culture in mouse. The cell cleavage rate reached to 2-4 cell stage at 24 hours of culture is 56.81% (50/88) and 48.21%(54/112) respectively, in PAF treated group which is added PAF on coculture and in coculture group. But the rate of cells cleavage was similar in both group after 48 hours of culture. The rate of unfertilization after insemination of oocytes was higher in coculture group(55..53%) than in PAF treated group(42.37%). And in assessment of undeveped embryos, the rate of equalized cell block was similar on both, coculture alone (35.3%)and PAF treated coculture(35.5%). while unequalized cell block was higher rate in PAF treated coculture(19.4%) than coculture alone (11.8%). But the rate of cytoplasmic degeneration of undeveloped embryos was significantly higher in PAF treated coculture than coculture alone. In conclusion, we have observed that PAF treated coculture is superior in the rates of in vitro fertilization and early embryo cell cleavage compared to those in coculture alone, but there is no difference on the rates of embryo develpments, cell degeneration, cell quality in both PAF treated coculture and coculture alone when the embryo cells were continuosly cultured for 48 hours or more.