Saccharomyces cerevisiae에서 Cycloinulooligosaccharide Fructanotransferase 유전자의 표층 발현

김현진,이재형,김현철,김연희,권현주,남수완,Kim, Hyun-Jin,Lee, Jae-Hyung,Kim, Hyun-Chul,Kim, Yeon-Hee,Kwon, Hyun-Ju,Nam, Soo-Wan 한국생명과학회 2007 생명과학회지 Vol.17 No.2

Paenibacillus macerans 유래의 cycloinulooligosaccharide fructanotransferase (CFTase) 유전자(cft, 2832 bp, 103.8 kDa)를 효모 표층발현 vector인 pCTcon (GAL1 promoter)에 subcloning 하였다. 구축된 pCTECFTN (9.0 kb)를 숙주세포인 s. cerevisiae EBY100에 형질전환한 후, uracil이 결핍된 배지와 inulin 함유배지에서 선별하였다. cft 유전자는 GAL1 promoter에 의해 효모 형질전환체에서 성공적으로 발현되었다. inulin으로부터 cyclofructans (CFs)로 생산하는 효소적 능력으로부터 표층발현 유무를 확인하였다. YPDG배지에서 48시간 배양 후 분획한 균체는 5.52 unit/ l 의 활성을 보였다. CF 생산을 위한 효소의 최적 반응 조건으로 pH 8.0, 반응온도 $50^{\circ}C$, 기질농도 5%, 기질은 Jerusalem artichoke 등의 inulin과 표층 발현 CFTase 효소반응 결과, cycloinulohexaose (CF6), cycloinuloheptaose (CF7), 그리고 cycloinulooctaose (CF8)이 생성되었고, 이 중에서 CF6가 주 생성물이었다.

Burden of Disease Due to Outdoor Air Pollution in Korea: Based on PM₁₀

김현진,윤석준,김형수,이건세,김은정,조민우,오인환,Kim, Hyun-Jin,Yoon, Seok-Jun,Kim, Hyeong-Su,Lee, Kun-Sei,Kim, Eun-Jung,Jo, Min-Woo,Oh, In-Hwan Korean Society of Environmental Health 2011 한국환경보건학회지 Vol.37 No.5

Purpose: This study measured the burden of disease in Korea related to outdoor air pollution using disability-adjusted life year (DALY). Materials and Methods: As a risk factor of outdoor air pollution, particulate matter with a diameter less than 10 ${\mu}m$($PM_{10}$) was used. First, $PM_{10}$-related diseases and their relative risk (RR) were selected by means of a literature review. Second, population attributable fractions were computed by using formulae including RR and population exposure to $PM_{10}$. Third, DALYs of $PM_{10}$-related diseases in Korea were estimated. Finally, the attributable burden of disease due to $PM_{10}$ was measured as the sum of the products that multiplied the DALYs of $PM_{10}$-related diseases by their population attributable fractions. Results: The disease burden of PM10 was 6.9 DALY per 1,000 persons in 2007. The attributable burden of $PM_{10}$ was 2.68 for lung cancer, 2.41 for COPD, 0.62 for ischemic heart disease, 0.61 for pneumonia, 0.55 for asthma, and 0.03 for preterm. Conclusions: This study showed the environmental burden of disease of $PM_{10}$ and burden of $PM_{10}$-related disease through objective data. It also suggested that active efforts are needed to continuously measure and reduce the burden of environmental diseases in Korea.

감모후유증(感冒後遺症)으로 기허형(氣虛型)호흡기 질환을 호소하는 환자의 한약치료에 관한 임상연구

김현진,한효정,장정아,박은영,안태한,서호석,김진원,Kim, Hyun-Jin,Han, Hyo-Jung,Jang, Jeong-A,Park, Eun-Young,An, Tae-Han,Seo, Ho-Seok,Kim, Jin-Won 대한한의학방제학회 2010 대한한의학 방제학회지 Vol.18 No.1

Objectives : In order to confirm a remedial effect and related influence of the clinic treatment by prescribing Samchuljojung-tang(蔘朮調中湯), Bojungikqi-tang(補中益氣湯) and Samso-um(蔘蘇飮) to patients who suffering from a chronic cough and rhinorrhea. Methods : 1. In a clinical observation and analysis method, we have selected the 23 patients who have visited National Oriental Medical Center, date from 2007.8.21 to 2008.7.31 and suffering from a chronic cough and rhinorrhea caused by the common cold sequelae. 2. Separated them into three types(I: cough type, II: rhinorrhea type, III: cough & rhinorrhea type). 3. Observed thoroughly the level of symptom and recorded it for each type with questionnaire survey at the beginning set 4. Prescribed Samchuljojung-tang, Bojungikqi-tang and Samso-um for type I, II, III respectively. 5. Observed the level of symptom and treatment afterwards affect, and recorded movements of intensity level for 3 types with same questionnaires. Results : 1. Type I : The survey turned out to be from 9 patients, initial level recorded 31.89 at the entry diagnosis, and next level that means changing of symptoms, recorded to 12.67 after prescribing herb medication. 2. Type II : From 11 patients, initial level was 32.82 and next level was 13.45. 3. Type III : From 3 patients, initial level was 37.67 and next one was 16.00. Conclusions : It is concluded that there is a significant remedial effect and related influence of the clinic treatment for each type of patients who suffering from a chronic cough and rhinorrhea caused by the common cold sequelae.

Nd:YAG 레이저에 의한 다층 박막의 미소 점 마킹

김현진,신용진,Kim, Hyun-Jin,Shin, Yong-Jin 한국광학회 2004 한국광학회지 Vol.15 No.4

We separated the multilayer structure of CD-R(compact disk-recordable) and investigated optimal spot marking conditions and physical and chemical transitions in response to various laser beam energh levels. Spot marking(80 ${\mu}{\textrm}{m}$ spot size) was produced on the surface of each layer using a Q-switched Nd:YAG laser between 27 mJ and 373mJ. By investigating resulting pit formation with Optical Microscopy(OM) and Optical Coherence Tomography(OCT), we analyzed the formation process of spot marking in the multilayer structure of different chemical composition. The localized heating of the substrate in the multilayer thin film caused the short temporal thermal expansion, and absorbed optical energy between reflective and dye interfaces melted dye and increased the volume. During the cooling phase, formation of pit and surrounding rim can be explained by three distinct processes; effect of surface tension, evaporation by spontaneous temperature increase due to laser energy, and mass flow from the recoil pressure. Our results shows that the spot marking formation process in the multilayer thin film is closely related to the layers' physical, chemical, and optical properties, such as surface tension, melt viscosity, layer thickness, and chemical composition.

식품분야에서 Systems Biology 기술의 응용

김현진,Kim, Hyun-Jin 한국식품연구원 2011 食品技術 Vol.24 No.2

Plant Stress Response를 이용한 과채류의 Phytochemicals 함량 증가 기술

김현진,Kim, Hyun-Jin 한국식품연구원 2009 食品技術 Vol.22 No.4

커뮤니티를 고려한 주거환경개선요소 설정 및 중요도 분석

김현진,구자훈,원제무,Kim, Hyun-Jin,Koo, Ja-Hoon,Won, Jai-Mu 한국주거학회 2009 한국주거학회 논문집 Vol.20 No.6

This study initially analyzes the meaning of Community-Based Residential Environment Improvement. A residential environment improvement factor was determined through an analysis of the function, components, Planning techniques of the community, the evaluation criteria of the residential environment, and through relative literature documenting plans for the adjustment of urban and housing environments. In terms of the methodological approach, decisions were made based on the results of an expert focus-group-interview, a goodness-of-fit test, and by using the analytic network decision-making method from which the correlation among items can be considered. Results of this analysis show that the, order of importance of factors for a community-based residential environment is as follows: interactional character, cognitive character, and locational character. Furthermore, the most important aspect for each of these factors are the neighbor-hood relationship in terms of the interactional character, common consciousness for the cognitive character and place for locational character. The results of this study can be used by linking them with questionnaire items for basic resident research when a residential environment improvement project or a plan for the adjustment of urban and housing environments is planned.

해외 기술: Metabolomics 기술을 활용한 식품의 건강 기능성 연구

김현진,Kim, Hyun-Jin 한국식품연구원 2010 食品技術 Vol.23 No.1

러-우 전쟁 이후 NATO의 전략 변화와 한국의 안보전략적 함의 고찰

김현진,배일수,Kim Hyun Jin,Bae Il Soo 국제문화기술진흥원 2024 문화기술의 융합 Vol.10 No.3

NATO는 러시아-우크라이나 전쟁 이후 그동안 유지해오던 평화유지 전략에서 탈피하고 다자안보협의체 강화, 전략적 안정성 추구, 군비 확충, 협력안보 및 인도-태평양 지역까지 대화·협력을 강화할 것을 천명하였다. NATO의전략 변화는 직·간접적으로 한국의 안보환경에 영향을 미친다. 이를 명확히 분석하고 대응할 수 있는 정책 및 전략을수립해야 위협을 억제하고 국가이익을 보호할 수 있다. NATO는 중국과 러시아를 위협으로 명시하고 있다. 우리는NATO와 협력과 연대를 기회로 삼아 소다자 안보협력 및 동맹 강화, 방산시장 개척 및 확대, 우크라이나 재건사업참여 기회 확대를 얻을 수 있을 것이다. After the Russia-Ukraine War, NATO broke away from the peacekeeping strategy that it had maintained and declared that it would strengthen the multilateral security consultative body, pursue strategic stability, expand armaments, cooperate security, and strengthen dialogue and cooperation in the Indo-Pacific region. Changes in NATO's strategy directly and indirectly affect Korea's security environment. Only by clearly analyzing this and establishing policies and strategies to respond to it can the threat be suppressed and national interests protected. NATO designates China and Russia as threats. By using cooperation and solidarity with NATO as an opportunity, we will be able to strengthen security cooperation and alliances, develop and expand the defense industry market, and expand opportunities to participate in the Ukraine reconstruction project.

다중 주사 경로 회로 기판을 위한 내장된 자체 테스트 기법의 연구

김현진,신종철,임용태,강성호,Kim, Hyun-Jin,Shin, Jong-Chul,Yim, Yong-Tae,Kang, Sung-Ho 대한전자공학회 1999 電子工學會論文誌, C Vol.c36 No.2

The IEEE standard 1149.1, which was proposed to increase the observability and the controllability in I/O pins, makes it possible the board level testing. In the boundary-scan environments, many shift operations are required due to their serial nature. This increases the test application time and the test application costs. To reduce the test application time, the method based on the parallel opereational multiple scan paths was proposed, but this requires the additional I/O pins and the internal wires. Moreover, it is difficult to make the designs in conformity to the IEEE standard 1149.1 since the standard does not support the parallel operation of data shifts on the scan paths. In this paper, the multiple scan path access algorithm which controls two scan paths simultaneously with one test bus is proposed. Based on the new algorithm, the new algorithm, the new board level BIST architecture which has a relatively small area overhead is developed. The new BIST architecture can reduce the test application time since it can shift the test patterns and the test responses of two scan paths at a time. In addition, it can reduce the costs for the test pattern generation and the test response analysis. 인쇄 회로 보드 수준의 테스팅을 위해 제안된 IEEE 표준 1149.1은 보드상의 테스트 지점에 대한 제어용이도와 관측용이도를 향상시켜 보드의 테스트를 용이하게 해준다. 그러나, 경계 주사 환경에서는 테스트 입력과 테스트 결과에 따른 데이터가 하나의 주사 연결에 의해서 직렬로 이동된다. 이는 테스트 적용시간을 증가시키고 따라서 테스트에 드는 비용을 증가시킨다. 테스트에 소모되는 시간을 줄이기 위해 병렬로 다중주사 경로를 구성하는 방법이 제안되었다. 하지만 이는 여분의 입출력 핀과 내선을 필요로 한다. 더구나 IEEE 표준 1149.1은 주사 경로 상에 있는 IC들의 병렬 동작을 지원하지 않기 때문에 표준에 맞게 설계하기가 어렵다. 본 논문에서는 하나의 테스트 버스로 두 개의 주사 경로를 동시에 제안하는 다중 주사 경로 접근 알고리즘에 기초하여 적은 면적 오버헤드를 가지고 빠른 시간 내에 보드를 테스트할 수 있는 새로운 보드수준의 내장된 자체 테스트 구조를 구현하였다. 제안된 내장된 자체 테스트 구조는 두 개의 주사 경로에 대한 테스트 입력과 테스트 결과를 이동시킬 수 있으므로 테스트에 소모되는 시간을 줄일 수 있고 또한 테스트 입력의 생성과 테스트 결과의 분석에 소모되는 비용을 줄일 수 있다.