치아 회분말과 연석고 매식 후 골 재생 과정에서 Transforming growth factor-β1의 발현

허영철 조선대학교 대학원 2005 국내박사

Expression of Transforming growth factor-β1 in bone regeneration after the implantation of particulate dentin and plaster of Paris Huh Young Chul Advisor : Prof., Su-Gwan Kim DDS, Ph.D. Department of Dentistry, Graduate School of Chosun University This study was performed to investigate the expression of growth factor transforming growth factor (TGF)-β1 in a rat calvarium defect model using particulate dentin and/or plaster of Paris, and correlate the bone regeneration process with the histologic events. Thirty-two Sprague-Dawley rats were divided into 4 groups of 8 animals each. A 1.0 cm-sized calvarial defects were made and the defect was filled with different graft materials as follows : Group A, the defects were filled with a mixture of particulate dentin and plaster of Paris with a 2:1 ratio; Group B, the defects were filled with plaster of Paris only; Group C, defects were filled with particulate dentin only; Group D, untreated control group. The animals were sacrificed at 1, 2, 4, 8 weeks after implantation. Excised wound tissues were processed for histology, immunohistochemistry and RT-PCR for the analysis of TGF-β1 expression. Gene expression of TGF-β1 was detected for all experimental groups. The highest gene expression was observed in the specimen taken at the first week after implantation in Group A. According to the histologic and immunohistochemical studies, TGF-β1 positive osteoblast-like cells were found in the early stage of healing after the implantation of particulate dentin and plaster of Paris. These findings suggest that TGF-β1 may be related to new bone formation at the early healing process after the implantation of particulate dentin and plaster of Paris.

성견에서 이산화탄소 레이저 조사후 임프란트의 표면 변화

양석진 조선대학교 대학원 2006 국내석사

Not only the implantation itself but also the long term stability of the implant became a matter of concern because of the popularization of implantation. A lot of treatment method has been offered. But it doesn't seem that the clinical research plan has been established. It is necessary to remove the contaminant effectively and reduce the damage of implant surface and soft tissue during the procedure. The proper use of CO₂ laser can minimize the problems above and can be used to treat peri-implantitis. The purpose of this study was to establish a standard irradiation amount of CO₂ laser. It was made from the measurement of the temperature change of the surface and the micro-changes of the surface when the osseointegrated impalnts of the adult dogs were exposed to the CO₂ laser of 1, 2, 3, 5 W of output. The peri-implantitis can be effectively treated by CO₂ laser of 2∼5W output without any damages on the surface of the implant.

성견에서 하악골 수평골신장술 후 임플란트 지연 식립시 조직형태계측학적 평가

선주림 조선대학교 대학원 2006 국내석사

Purpose : This study was aimed to evaluate osseointegration at implantation after consolidation in the distracted narrow alveolar bone. Materials and Methods : Three adult mongrel dogs, weighting about 9-10kg, were used in this study. The lower premolars were extracted and horizontal distraction was performed using distraction device after 8 weeks. 8 weeks after distraction, screw-type implants is installed. The dogs are sacrificed after 4 weeks. Results : Direct bone contact is achieved and there were no significant difference between control group and experimental group at implantation area after 4 weeks. Conclusion : Implantation after horizontal distraction in narrow alveolar ridge is useful technique.

임프란트 수술환자의 만족도

김정선 조선대학교 대학원 2006 국내석사

Introduction The satisfaction of implant patients, the quality of life, and the self-esteem were evaluated by self-administered questionnaire. And the correlations among them were investigated in this study. Methods The questionnaires composed of 66 questions on socio-demographic factors, quality of life, self-esteem, service of the dental hospital, and the implant surgery itself were given 91 subjects. Results The correlation between the quality of life and self-esteem was statistically significant (group A). And the correlation between the satisfaction at implant surgery itself and that at the service of the dental hospital was statistically significant, too (group B). But, the correlation between group A and B did not have statistical significance. Conclusion Continuous studies are necessary for objective analysis of patients' subjective responses and establishment of systematic theses.

백서에서 초기 골 형성에 미치는 키토산과 치아회분말의 효과

박순식 조선대학교 대학원 2006 국내석사

Purpose : The purpose of this study was to evaluate the healing effect of grafting materials after implantation of chitosan and tooth-ash mixture. Material and Method : The critical size defect of rat calvarium, 8mm size in diameter, was used and 75 rats were divided into 5 experimental groups. The control group was performed only artificial bone defect in the frontal area of rat and experimental group was divided 4 parts. The experimental group 1 was grafted with tooth-ash, the experimental group 2 was grafted with pig tooth-ash and plaster of Paris mixture, experimental group 3 was grafted with pig tooth-ash and chitosan combination, experimental group 4 was grafted with chitosan only. The experimental rats were sacrificed at the 2nd , 4th and 8th weeks after operation. Result : The results were as follows ; More significant new bone forming activity was found in all experimental group than control group. In all groups, there were more significant new bone forming activity was found in 8 weeks group than 4 weeks group. There were slightly more new bone forming activity in group 3. Conclusion : These results suggested that the mixture of chitosan and tooth-ash might be effective materials for new bone formation in osseous defects.

오염된 자가치아 뼈이식재의 처치방법에 따른 골형성능 비교

진수영 조선대학교 대학원 2012 국내석사

Purpose The purpose of this study was to evaluate new bone formation and bone to implant contact histomorphometrically using contaminated autogenous teeth bone graft materials which was re-sterilized with EO gas, autoclave and adapted to patients having alveolar bone defect during implant placement procedure. Method The experiment was conducted using Dentis�� HAPTITE 3.7mm diameter,10mm length implant and autogenous teeth bone graft aimed at bred 6 hybrid dogs with about 15kg aging 6 to 12 months under same condition. The experiment group was composed to no treatment group, sterilization using EO gas group, and sterilization using autoclave group according to sterilizing method after contamination of autogenous teeth bone graft. Experimented animals were sacrificed after 4 weeks and 8 weeks of post-experiment, clinical evaluation was done during osteocomma collection, and bone to implant contact ratio(BICR) and new bone formation ratio(NBFR) were evaluated using the slides made from collected tissues. Results The result of bone to implant contact ratio (BICR) of dressing-free group and the sterilization using autoclave group in 4 weeks group showed lower level versus control group, the sterilization using EO gas group, however, it compare to difference with control group. And the result of the dressing-free group in 8 weeks group resulted in lower proportion of implant bone contact, yet the result was not statistically significant. The new bone formation ratio inside bone defect area of conta- minated group and the sterilization using autoclave group in 4 weeks group resulted in lower level versus control group, the sterilization using EO gas group, however, showed no-regardful difference with control group. And in 8 weeks group, the sterilization using autoclave group resulted in significant lower level versus control group, however, other experiment groups showed no-regardful difference. Conclusion According to the above result, the sterilization using ethylene oxide gas might be considered favorable than high pressure sterilization to the new bone formation and implant osseo-integration in case of re-use of contaminated autogenous teeth bone graft materials.

배양 상아질모세포 성장과 세포외 기질형성에 미치는 resveratrol의 효과

신상훈 조선대학교 대학원 2016 국내석사

Resveratrol (3,4',5,-trihydroxystilbene), a phytoalexin that is present in grapes, exerts a variety of actions to reduce superoxides, prevent diabetes mellitus and inhibit inflammation. Furthermore, resveratrol acts as a chemopreventive agent and induces apoptotic cell death in various types of cancer cells. However, the role of resveratrol in odontoblastic cell differentiation is still unclear. In this study, therefore, the effect of resveratrol on regulating odontoblast differentiation was examined in MDPC-23 mouse odontoblastic cells derived from mouse dental papilla cells.

혈소판 풍부 피브린이 골재생에 미치는 효과

유재식 조선대학교 대학원 2016 국내박사

임플란트 식립을 위해서는 우선 골질과 골양이 풍부해야 하는데, 최근 많은 양의 골과 양질의 골을 얻기위해 다양한 연구들이 이루어지고 있다. 골재생시 많은 양의 골과 양질의 골을 얻기 위해서 최근 골 형성을 촉진시키는 물질을 적용하는 시도가 이루어지고 있다. 골 형성을 촉진시키는 물질로는 골유도단백질(bone morphogenic protein, BMP), 혈소판 유래 성장인자(platelet derived growth factor, PDGF), 형질 전환 성장인자(transforming growth factor, TGF)등이 있으며, 이러한 성장인자는 혈소판이 분해되면서 다량 분비하게 된다. 이에 착안하여 본 연구에서는 PRF를 이용하여 PRF내의 성장인자가 조골 세포의 증식과 분화에 어떤 영향을 미치는지 알아보고, 성견의 골 결손부위에 골 이식술을 시행했을 때 PRF의 효과를 알아보고자 하였다. 그 결과 PRF는 MG-63세포의 ALP 활동과 석회화 및 광화를 촉진시키며, 골형성과 관계된 생체지표 유전자 역시 증폭되는 것을 확인하였다. 성견에서도 방사선학적, 조직학적 분석을 한 결과 2주군에서 PRF를 섞은 bio-oss를 이식한 실험군 II에서 bio-oss만을 이식한 실험군 I과 대조군에 비해 신생골 형성이 증가되는 것을 관찰하였다. 이에 PRF는 골 재생에 매우 큰 효과가 있다는 결론을 내렸으며, 간단한 방법으로 얻을수 있는 훌륭한 골이식 첨가제라는 것을 보여주었다.

Sema6A 표적작용을 통한 microRNA-203의 구강암세포 성장 억제

임형섭 조선대학교 2017 국내박사

MicroRNA(miRNA)는 21-25개의 염기서열로 구성된 noncoding RNA 분자로, 표적 유전자의 3’-UTR 부위에 상보적인 염기서열을 가지고 그 표적유전자의 단백합성을 억제시키거나 촉진시킨다. Oncogenic miRNA는 종양과 관련된 miRNA로 최근 여러 종양에서 oncogenic miRNA 발현 분석을 통해 다양한 종양의 조기진단, 예후측정에 응용가능성을 시사하고 있다. 본 연구에서는 구강암세포에서 miRNA들의 발현을 비교․분석하여 구강암 특이 miRNA를 확립하고, miRNA-203 제어에 의한 종양유전자 Sema6A 표적작용을 통한 구강암세포 성장억제의 분자적 기전을 밝히고자 하였다. 구강암세포에서 miRNA-203 제어에 의한 구강암세포 성장억제 작용기전을 규명하기 위해 사람 구강암세포주 YD-38에 miRNA-203을 처리한 후, miRNA microarray 분석, PCR 분석, 세포증식 분석, 세포사멸 분석, immunoblotting 및 luciferase activity 분석 등을 시행하여 다음과 같은 결과를 얻었다. 1. 정상 사람 구강각화세포 NHOK에 비해 YD-38 구강암세포에서 miRNA-203의 발현이 현저히 감소하였다. 2. YD-38 세포에서 miRNA-203은 세포의 성장을 뚜렷이 억제시켰다. 3. YD-38 세포에서 miRNA-203은 세포 apoptosis를 유도하였다. 4. YD-38 세포에서 miRNA-203은 종양유전자 Sema6A의 발현을 억제하였다. 5. YD-38 세포에서 miRNA-203은 종양유전자 Sema6A를 억제함으로써 luciferase 활성을 감소시켰다. 본 연구의 결과로서 사람 구강암세포주 YD-38에서 miRNA-203은 종양억제 miRNA로써 기능을 하여 종양유전자 Sema6A의 발현을 감소시켜 구강암세포 증식억제 및 세포 apoptosis를 유도하는 것으로 사료된다. --------------------------------------------------------------------- 중심어: miRNA-203, 종양유전자, 구강암, Sema6A, 세포사멸

성견 하악골에서 다양한 드릴링 속도에 따른 임플란트 초기 골유착에 관한 비교 연구

서동욱 조선대학교 2016 국내석사

I. Purpose Successful osseointegration of implants can be influenced by the drilling speeds of implant installation. This study evaluated the effect of drilling speed on early bone healing in mandible of dogs. II. Material and methods Six young adult dogs were selected and two mandibular premolars and two molars were extracted from both sides. After two months of healing, 3 hydroxyapatite surfaced Ф3.7*10mm fixtures (OneQ, Dentis, Daegu, Korea) were implanted with drilling speeds of 50, 800, and 1200 rpm on the right side first and then the left side after 2 weeks. Implant stability quotient (ISQ) was measured upon insertion, and after 4 weeks for the right side, and after 2 weeks for the left side using Osstell Mentor®. The animals were euthanized after allowing 4 weeks and 2 weeks of healing for the right and left side of the mandible, respectively. The implant and surrounding tissue were histologically analyzed. III. Results The implants were stable upon insertion for both groups and although implant stability steadily increased for 4 weeks, no statistically significant differences were observved between the surgical protocols. From histomorphological evaluation, a significant increase from 2 to 4 weeks was observed for all groups for bone-implant contact(BIC), whereas no significant differences were detected between 2 and 4 weeks for the 50 and 800 rpm groups. Based on the ISQ measurement, all implants were measured higher than 60 for each drilling speed from both 2 and 4 weeks group. The 1200 rpm group showed a higher value than the 50 rpm group at 2 weeks and 4 weeks. (p<0.05) From the results of a histomorphometric analysis, new bone formation around the implant was highest for the 800 rpm group at 2 weeks and the 1200 rpm group at 4 weeks. The BIC of the superior half of the alveolar bone was highest for the 800 rpm group at 2 weeks and the 1200 rpm group at 4 weeks. There was no statistically significant differences. IV. Conclusion This study suggests that 50, 800, and 1200 rpm are drilling speeds which can expect favorable outcome, yet, higher drilling speed presented overall the best biological responses. However, further studies are needed for statistical significance.