http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Kim, J.E.,Kim, H.J.,Pandit, S.,Chang, K.W.,Jeon, J.G. Inverni Della Beffa S.p.A ; Elsevier Science 2011 Fitoterapia Vol.82 No.3
Rheum undulatum root has been used traditionally in Korea for the treatment of dental diseases. The purpose of this study was to separate a fraction from R. undulatum showing anti-acid production activity against Streptococcus mutans biofilms and identify the main components in that fraction. Methanol extract of R. undulatum root and its fractions were prepared. To select a fraction exhibiting anti-acid production activity, suspension glycolytic pH-drop assay was performed. Among the fractions tested, dichloromethane fraction exhibited the strongest activity in a dose-dependent manner. To examine the effect of the selected fraction on the anti-acid production of S. mutans biofilms, 74h old S. mutans biofilms were used. The selected fraction reduced the initial rate of acid production of S. mutans biofilms at sub-minimum inhibitory concentration (MIC) levels. HPLC qualitative analysis of the selected fraction indicated that the presence of anthraquinone derivatives, such as aloe-emodin, emodin, chrysophanol and physcion, as main components.
Ban, S.H.,Kwon, Y.R.,Pandit, S.,Lee, Y.S.,Yi, H.K.,Jeon, J.G. Inverni Della Beffa S.p.A ; Elsevier Science 2010 Fitoterapia Vol.81 No.1
Polygonum cuspidatum root has been traditionally used for the treatment of dental diseases in Korea. The purpose of this study was to evaluate effects of P. cuspidatum root on the development of dental caries, especially its effects against bacterial viability and caries-inducing factors of Strptococcus mutans and Strptococcus sobrinus. Among methanol extract of P. cuspidatum root and its fraction tested, ethyl acetate fraction, composed of polydatin, resveratrol, anthraglycoside B, and emodin, showed inhibitory effects on glycolytic acid production and glucosyltransferase activity of S. mutans and S. sobrinus in addition to antibacterial activities.
Raghavendran, H.R.B.,Rekha, S.,Cho, H.K.K.J.H.,Jang, S.S.,Son, C.G. Inverni Della Beffa S.p.A ; Elsevier Science 2012 Fitoterapia Vol.83 No.6
Panax ginseng is an indigenous medicinal herb and has traditionally been used among Asian population for relief of many human ailments. We investigated the prophylactic role of Korean P. ginseng extract (KG) against X-ray irradiation-induced emesis in an acute rat pica model. Rats were treated with KG (12.5, 25, 50mg/kg orally at -48, -24 and 0h) prior to X-ray irradiation (6Gy), and intake of kaolin and normal food and body weight changes examined as an index of the acute emetic stimulus. Levels of serotonin in small intestine tissue were assessed and histopathology of gastric tissue, small intestine and colon examined specific staining. Pre-treatment with KG (12.5 and 25mg/kg) reduced X-ray irradiation-induced kaolin intake at 24h. Normal food intake was improved in rats treated with 25mg/kg KG. The anti-emetic effect of KG was further confirmed on the basis of serotonin release, histopathological findings. Our findings collectively indicate that KG protects against X-ray irradiation-induced acute pica to a moderate extent, leading to improved feeding behavior in rats.
Choi, J.Y.,Kim, H.,Choi, Y.J.,Ishihara, A.,Back, K.,Lee, S.G. Inverni Della Beffa S.p.A ; Elsevier Science 2010 Fitoterapia Vol.81 No.8
Hydroxycinnamic acid amides of serotonin (HCAAS) [caffeoylserotonin (compound 1), p-coumaroylserotonin (compound 2), and feruloylserotonin (compound 3)] are secondary metabolites produced in plants. The aim of this study was to investigate the cytoprotective effects of HCAAS based on intracellular reactive oxygen radical (ROS) generation, lipid peroxidation, protein carbonylation, and phosphorylation of histone H2AX in H<SUB>2</SUB>O<SUB>2</SUB> treated-HepG2 and HaCaT cells. We have shown that HCAAS showed various strong antioxidant activities in hydrogen peroxide treated both cell lines, suggesting that these compounds may play as chemotherapeutic agents for preventing or reducing the oxidative stress-induced diseases.
3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase inhibitory effect of Vitis vinifera
Koo, M.,Kim, S.H.,Lee, N.,Yoo, M.Y.,Ryu, S.Y.,Kwon, D.Y.,Kim, Y.S. Inverni Della Beffa S.p.A ; Elsevier Science 2008 Fitoterapia Vol.79 No.3
Activity-guided fractionation of the methanol extract of Vitis vinifera bark led to the isolation of ε-viniferin, ampelopcin A, vitisin A and vitisin B. Vitisin A and vitisin B showed a remarkable inhibitory activity against 3-hydroxy-3-methylglutaryl-Coenzyme A (HMG-CoA) reductase with IC<SUB>50</SUB> value of 42.1 μM and 23.9 μM, respectively.
A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus
Kim, H.J.,Lee, J.Y.,Kim, S.M.,Park, D.A.,Jin, C.,Hong, S.P.,Lee, Y.S. Inverni Della Beffa S.p.A ; Elsevier Science 2009 Fitoterapia Vol.80 No.1
Calpains are calcium-dependent proteases that cleave a variety of intracellular substrates. The overactivation of μ-calpain is associated with a wide range of disease conditions. To search for calpain inhibitors from natural products, the phytochemical constituents of the ethyl acetate fraction of the whole plant of Orostachys japonicus (Crassulaceae) were studied. The various chromatographic separation of this fraction led to the isolation of a new tannin, (-)-epicatechin 5-gallate (1) along with 9 known compounds. Their structures were elucidated by spectroscopic and chemical analyses. Among them, (-)-epicatechin 5-gallate (1) and kaempferol (9) exhibited moderate inhibitory activity against μ-calpain with IC<SUB>50</SUB> values of 18.0+/-2.9 and 15.4+/-2.0 @?g/ml, respectively.
Characterization of a cinnamoyl derivative from broccoli (Brassica oleracea L. var. italica) florets
Survay, N.S.,Kumar, B.,Upadhyaya, C.P.,Ko, E.,Lee, C.,Choi, J.N.,Yoon, D.Y.,Jung, Y.S.,Park, S.W. Inverni Della Beffa S.p.A ; Elsevier Science 2010 Fitoterapia Vol.81 No.8
A new intact glucosinolate Cinnamoyl derivative [6'-O-trans-(4''- hydroxy cinnamoyl)-4-(methylsulphinyl) butyl glucosinolate] (A) has been isolated from Broccoli (Brassica oleracea L. var. italica) florets. The compound was isolated and characterized by LC, MS-ESI, FTIR, <SUP>1</SUP>H and <SUP>13</SUP>C NMR as well as <SUP>1</SUP>H-<SUP>1</SUP>H COSY, DEPT 135<SUP>o</SUP> spectrometric experiments.
Kim, D.Y.,Park, Y.G.,Quan, H.Y.,Kim, S.J.,Jung, M.S.,Chung, S.H. Inverni Della Beffa S.p.A ; Elsevier Science 2012 Fitoterapia Vol.83 No.1
As part of our search for biologically active anti-osteoporotic agents that enhance the differentiation and mineralization of osteoblastic MC3T3-E1 cells, we identified the ginsenoside Rd as the most active compound among ginsenosides. In this study, we showed that Rd stimulates osteoblastic differentiation and mineralization, manifested by the up-regulation of differentiation markers (alkaline phosphatase and osteogenic genes) and von Kossa/Alizarin Red staining, respectively. Rd induces the mRNA expression of bone morphogenetic protein-2 (BMP-2) and the secretion of the corresponding protein into media in a concentration-dependent manner. The mRNA expression and enzyme activity of alkaline phosphatase (ALP) were suppressed when MC3T3-E1 cells were exposed to noggin, a BMP-2 antagonist. The level of phosphorylated AMP-activated protein kinase (pAMPK) protein was also up-regulated by Rd in a time- and concentration-dependent manner. Rd-induced ALP activity, mineralization, and BMP-2 production were all inhibited by either Ara-A (AMPK inhibitor) or siRNA targeting AMPK. In addition, we investigated whether Rd-induced BMP-2 transduces signals through the Smad signaling pathways. Rd induced a significant level of phosphorylation of Smad1/5, and this effect was blocked when the cells were transfected with siRNA targeting Smad4, indicating that Smad1/5 must form complex with Smad4 to translocate into the nucleus and regulate the transcription of osteogenic genes. In summary, these results indicate that Rd induces the differentiation and mineralization of MC3T3-E1 cells through the activation of the AMPK/BMP-2/Smad signaling pathways. These findings provide a molecular basis for the osteogenic effect of Rd in MC3T3-E1 cells.
Lee, G.S.,Hong, E.J.,Gwak, K.S.,Park, M.J.,Choi, K.C.,Choi, I.G.,Jang, J.W.,Jeung, E.B. Inverni Della Beffa S.p.A ; Elsevier Science 2010 Fitoterapia Vol.81 No.1
Chamaecyparis obtusa (C. obtusa) is a conifer in the cypress family Cupressaceae, native to northeast Asia. The essential oils of C. obtusa have antibacterial and antifungal effects and several products such as hygienic bands, aromatics, and shampoos contain these oils as a natural source of antimicrobial/antifungal agents. Interestingly, some consumers suffering from baldness and/or other forms of hair loss have reported a hair growth promoting effect of shampoos containing these oils. In the present study, the hair growth promoting effect of C. obtusa oils was elucidated in an animal model. C. obtusa oils promoted the early phase of hair growth in shaved mice. In addition, we examined the molecular effect of C. obtusa oils on the regulation of hair morphogenesis and hair growth using the human keratinocyte cell line HaCaT. In the current study of hair growth regulating genes, the expressions of vascular endothelial growth factor (VEGF), transforming growth factor (TGFβ1), and keratinocyte growth factor(KGF) have been analyzed by real-time PCR in HaCaT cells. The essential oils of C. obtusa were divided into seven fractions for treatment of HaCaT cells. VEGF transcripts were induced by fractions 6 and 7; however, TGFβ1 and KGF mRNA levels were unchanged by C. obtusa oils or fractions. Fraction 7 was separated into seven sub-fractions and studied further. Sub-fractions E and D significantly increased VEGF and KGF gene expression without up-regulating the hair growth inhibition factor, TGFβ1. The components of the two sub-fractions were further analyzed by gas chromatography and mass spectrometry. Cuminol, eucarvone, and calamenene were common to these two sub-fractions, although the effects of these individual components were not determined. Taken together, these results suggest that C. obtusa oils promote hair growth in an animal model and a positive regulator of hair growth, VEGF, was induced by particular components of these oils.
Kim, J.S.,Song, H.J.,Ko, S.K.,Whang, W.K.,Sohn, U.D. Inverni Della Beffa S.p.A ; Elsevier Science 2010 Fitoterapia Vol.81 No.2
Heme oxygenase-1 (HO-1) is one of the antioxidant enzymes which help protect against cellular damage. The present study examined the ability of Quercetin-3-O-β-d-glucuronopyranoside (QGC), flavonoid glucoside extracted from Rumex Aquaticus Herba, to induce expression of HO-1 and analyzed its signaling mechanism in cultured feline esophageal epithelial cells (EEC). Culture of the esophageal epithelial cells from cat was prepared. The data suggested that QGC could result in enhanced antioxidant enzyme defense system via HO-1 expression and Nrf2 translocation involving both the ERK and PI3K-Akt pathways as well as partly PKC pathways in EEC.