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F₁rat o''ztu''rk,Hasan Babacan,Ruhi Nalc.ac₁,Alper Kus.tarc₁ 대한치과교정학회 2009 대한치과교정학회지 Vol.39 No.6
Objective: The purpose of this study was to compare the shear bond strength (SBS) of brackets and microleakage of a tooth-adhesive-bracket complex bonded with a direct and an indirect bonding technique after thermocycling. Methods: Fifty non-carious human premolars were divided into two equal groups. In the direct bonding group a light-cured adhesive and a primer (Transbond XT) was used. In the indirect-bonding group, a light-cured adhesive (Transbond XT) and chemical-cured primer (Sondhi Rapid Set) were used. After polymerization, the teeth were kept in distilled water for 24 hours and thereafter subjected to thermal cycling (500 cycles). For the microleakage evaluation, 10 teeth from each group were further sealed with nail varnish, stained with 0.5% basic fuchsin for 24 hours, and examined under a stereomicroscope. Fifteen teeth from each group were used for SBS testing with the universal testing machine and adhesive remnant index (ARI) evaluation. Data were analyzed using the Mann-Whitney U test, Chi-square test, and Fisher's exact test. Results: There were no statistical differences on SBS and microleakage between the two bonding techniques. The indirect bonding group had a significantly lower ARI score. Bracket failures were obtained between enamel-resin interfaces. Conclusions: The type of bonding technique did not significantly affect the amount of microleakage and SBS. 본 연구는 직접 또는 간접 부착법으로 부착한 교정용 브라켓을 열순환 처리 후에 전단 결합 강도(shear bond strength, SBS)와 치아-접착제-브라켓 복합체의 미세 누출을 비교하기 위하여 시행하였다. 50개의 치아우식증이 없는 사람 소구치를 구하여 동등한 2개의 군으로 나누었다. 직접 부착군에서는 광중합 접착제 및 primer (Transbond XT)를 사용하였고 간접 부착군에서는 광중합 접착제와(Transbond XT) 화학중합 primer (Sondhi Rapid Set)를 사용하였다. 중합 후에 치아는 24시간 동안 증류수에 보관하였고 이후 500회 열순환 처리를 하였다. 미세누출의 평가를 위해 각 군에서 10개의 치아에 nail varnish로 추가 봉연을 실시하고 0.5% basic fuchsin에 24시간 동안 염색한 후 입체현미경으로 검경하였다. 만능시험기를 이용하여 각 군의 치아 15개로 SBS를 계측하였고 adhesive remnant index (ARI)를 평가하였다. 결과 자료는 Mann-Whitney U test, Chi-square test, Fisher's exact test로 통계 분석하였다. 직접 부착군과 간접 부착군 사이에 SBS 및 미세누출의 통계적으로 유의한 차이는 보이지 않았으며 간접 부착군에서 통계적으로 유의하게 ARI 점수가 낮았다. 브라켓의 탈락은 법랑질-레진 계면에서 발생하였다. 이상의 결과로 보아 부착법의 차이는 미세누출의 양과 SBS에 유의한 영향을 미치지 않는다.
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Biotransformation of propolis phenols by L. plantarum as a strategy for reduction of allergens
Hatice Kalkan Yıldırım,Erhan Canbay,S¸ ahin O¨ ztu¨rk,Ozan Aldemir,Eser Y. So¨zmen 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.6
Propolis is a natural product produced by honeybees. It has antioxidant effects as well as antimicrobial, antiseptic, antibacterial, anti-inflammatory and antimutagenic properties. Except these important healthy properties some cytotoxic effects causing allergies also have been reported. In this study have been evaluated changes of phenolic compounds including allergens molecules found in propolis. Before biotransformation, propolis samples were treated with different solvent (ethanol and polyethylene glycol) to facilitate solvation of solid samples. Biotransformation was done by three different strains of Lactobacillus plantarum (10, 8014, ATCC). Results demonstrated the importance of used solvent/ treatment for extraction procedure and strains of L. plantrum. The lowest values of main allergens were determined as 321 ng/mL for BCAFE, 320 ng/mL for 1.1 DMAECAFE and 8.02 ng/mL for CAPE. The study is the first work deal with evaluation of bioconversion of propolis by different L. plantarum strains and their effects on phenolic profile.
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Seo, Jungmok,Shin, Jung-Youn,Leijten, Jeroen,Jeon, Oju,Bal Ö,ztü,rk, Ayç,a,Rouwkema, Jeroen,Li, Yuancheng,Shin, Su Ryon,Hajiali, Hadi,Alsberg, Eben,Khademhosseini, Ali American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.16
<P>Biophysical cues can potently direct a cell’s or tissue’s behavior. Cells interpret their biophysical surroundings, such as matrix stiffness or dynamic mechanical stimulation, through mechanotransduction. However, our understanding of the various aspects of mechanotransduction has been limited by the lack of proper analysis platforms capable of screening three-dimensional (3D) cellular behaviors in response to biophysical cues. Here, we developed a dynamic compression bioreactor to study the combinational effects of biomaterial composition and dynamic mechanical compression on cellular behavior in 3D hydrogels. The bioreactor contained multiple actuating posts that could apply cyclic compressive strains ranging from 0 to 42% to arrays of cell-encapsulated hydrogels. The bioreactor could be interconnected with other compressive bioreactors, which enabled the combinatorial screenings of 3D cellular behaviors simultaneously. As an application of the screening platform, cell spreading, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) were characterized in 3D gelatin methacryloyl (GelMA) hydrogels. Increasing hydrogel concentration from 5 to 10% restricted the cell spreading, however, dynamic compressive strain increased cell spreading. Osteogenic differentiation of hMSCs was also affected by dynamic compressive strains. hMSCs in 5% GelMA hydrogel were more sensitive to strains, and the 42% strain group showed a significant increase in osteogenic differentiation compared to other groups. The interconnectable dynamic compression bioreactor provides an efficient way to study the interactions of cells and their physical microenvironments in three dimensions.</P> [FIG OMISSION]</BR>
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Bülent Çetin,Hakan Özer,Ahmet Cakir,Tas¸kin Polat,Atilla Dursun,Ebru Mete,Erdog˘an O¨ ztu¨rk,Melek Ekinci 한국식품영양과학회 2010 Journal of medicinal food Vol.13 No.1
The objective of this study was to determine the chemical compositions of the essential oil and hexane extract isolated from the inflorescence, leaf stems, and aerial parts of Florence fennel and the antimicrobial activities of the essential oil, hexane extract, and their major component, anethole, against a large variety of foodborne microorganisms. Gas chromatography and gas chromatography-mass spectrometry analysis showed that the essential oils obtained from inflorescence, leaf stems, and whole aerial parts contained (E)-anethole (59.28–71.69%), limonene (8.30–10.73%), apiole (trace to 9.23%), β-fenchyl acetate (3.02–4.80%), and perillene (2.16–3.29%) as the main components. Likewise, the hexane extract of the plant sample exhibited a similar chemical composition, and it contained (E)-anethole (53.00%), limonene (27.16%), γ-terpinene (4.09%), and perillene (3.78%). However, the hexane extract also contained less volatile components such as n-hexadecanoic acid (1.62%), methyl palmitate (1.17%), and linoleic acid (1.15%). The in vitro antimicrobial assays showed that the essential oil, anethole, and hexane extract were effective against most of the foodborne pathogenic, saprophytic, probiotic, and mycotoxigenic microorganisms tested. The results of the present study revealed that (E)-anethole, the main component of Florence fennel essential oil, is responsible for the antimicrobial activity and that the essential oils as well as the hexane extract can be used as a food preservative. This study is the first report showing the antimicrobial activities of essential oil and hexane extract of Florence fennel against probiotic bacteria.
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