Lactobacillus aquaticus sp. nov., isolated from a Korean freshwater pond.

Mañ,es-Lá,zaro, Rosario,Song, Jaeho,Pardo, Isabel,Cho, Jang-Cheon,Ferrer, Sergi Society for General Microbiology 2009 International journal of systematic and evolutiona Vol.59 No.9

<P>A Lactobacillus strain, IMCC1736T, was isolated recently from a Korean freshwater pond following an extensive study of the microbial community in this ecosystem. Its 16S rRNA gene was sequenced and phylogenetic analysis placed this strain within the Lactobacillus salivarius group, closely related to Lactobacillus satsumensis NRIC 0604T, with 97.9% sequence similarity. In the present work, the taxonomic status of strain IMCC1736T has been re-evaluated. It was characterized phylogenetically, genotypically and phenotypically and, based on DNA-DNA hybridization values, this strain represents a novel Lactobacillus species. Strain IMCC1736T can be differentiated genotypically from its closest relatives by randomly amplified polymorphic DNA analysis and ribotyping patterns; phenotypically, it can be distinguished by its inability to grow in 5% NaCl and at pH 3.3 and by certain carbohydrate fermentations. Strain IMCC1736T is Gram-positive, catalase-negative and microaerophilic. Cells are motile rods and show homofermentative metabolism. The name Lactobacillus aquaticus sp. nov. is proposed, with strain IMCC1736T (=CECT 7355T=DSM 21051T) as the type strain.</P>

Multistep electron transfer systems based on silicon phthalocyanine, [60]fullerene and trinitrofluorenone

Martí,n-Gomis, Luis,Ohkubo, Kei,Ferná,ndez-Lá,zaro, Fernando,Fukuzumi, Shunichi,Sastre-Santos, Á,ngela Royal Society of Chemistry 2010 Chemical communications Vol.46 No.22

<P>The synthesis and photodynamics in the absence and in the presence of Mg<SUP>2+</SUP> ions of a novel TNF-C<SUB>60</SUB>-SiPc-C<SUB>60</SUB>-TNF pentad are reported. The redox gradient approach allows to obtain a long-lived CS state of 160 ns and 200 μs in the absence and in the presence of Mg<SUP>2+</SUP> ions, respectively.</P> <P>Graphic Abstract</P><P>The redox gradient approach in a TNF-C<SUB>60</SUB>-SiPc-C<SUB>60</SUB>-TNF pentad allows the generation of a long-lived CS state of 160 ns/200 μs in the absence/presence of Mg<SUP>2+</SUP> ions respectively. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c002077j'> </P>

Rational design of a phthalocyanine–perylenediimide dyad with a long-lived charge-separated state

Blas-Ferrando, Vicente M.,Ortiz, Javier,Bouissane, Latifa,Ohkubo, Kei,Fukuzumi, Shunichi,Ferná,ndez-Lá,zaro, Fernando,Sastre-Santos, Á,ngela The Royal Society of Chemistry 2012 Chemical communications Vol.48 No.50

<P>A new ZnPc–PDI dyad presenting for the first time a charge-separated state lower in energy than the triplet excited state of the ZnPc and PDI has been synthesized. The rational design implies the substitution of the ZnPc with phenoxy groups and the bay substitution of the PDI with sulfonyl substituents. The lifetime of the charge-separated state was 72 μs.</P> <P>Graphic Abstract</P><P>The longest charge-separated state so far, 72 μs, without the addition of external components, for phthalocyanine–perylenediimide arrays has been described. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cc31087b'> </P>

Supramolecular Zinc Phthalocyanine-Imidazolyl Perylenediimide Dyad and Triad: Synthesis, Complexation, and Photophysical Studies

Cé,spedes-Guirao, F. Javier,Ohkubo, Kei,Fukuzumi, Shunichi,Ferná,ndez-Lá,zaro, Fernando,Sastre-Santos, Á,ngela Wiley (John WileySons) 2011 Chemistry, an Asian journal Vol.6 No.11

<P>Two new supramolecular architectures based on zinc phthalocyanine (Pc) and imidazolyl-substituted perylenediimide (PDI), ZnPc/DImPDI/ZnPc 1 and ZnPc/ImPDI 2, have been prepared. A strong electron-donor, ZnPc-8, which contained eight tert-octylphenoxy groups was synthesized to ensure high solubility, thereby reducing aggregation in solution and providing ??-donor features while avoiding regioisomeric mixtures. Also, PDI units were functionalized with tert-octylphenoxy groups at the bay positions, which provide solubility to avoid aggregation in solution, together with one and two imidazole moieties in the amide position, PDI-6 and PDI-4, respectively, to be able to strongly coordinate with the ZnPc complex. Supramolecular complexation studies by (1)H NMR spectroscopy and ESI-MS demonstrate a high coordinative binding constant between imidazole-substituted PDI-4 or PDI-6 and ZnPc-8. The same results were confirmed by UV/Vis and fluorescence titration studies. UV/Vis titration studies revealed the formation of a 1:1 complex ZnPc/ImPDI 2 for the systems ZnPc-8 and PDI-6 and a 2:1 complex ZnPc/DImPDI/ZnPc 1 for the interaction of ZnPc-8 and PDI-4. The binding constant in both cases was determined to be on the order of 10(5) M(-1). Femtosecond laser flash photolysis measurements provided a direct proof of the charge-separated state within both supramolecular assemblies by observing the transient absorption band at 820 nm due to the zinc phthalocyanine radical cation. The lifetimes of charge-separated states are (9.8±3) ns for triad 1 and (3±1) ns for dyad 2. As far as we know, this is the first time that a radical ion pair has been detected in a supramolecular assembled ZnPc-PDI system and has obtained the longest lifetime of a charge-separated state published for ZnPc-PDI assemblies.</P>

Light harvesting zinc naphthalocyanine–perylenediimide supramolecular dyads: long-lived charge-separated states in nonpolar media

El-Khouly, Mohamed E.,Gutié,rrez, Ana M.,Sastre-Santos, Á,ngela,Ferná,ndez-Lá,zaro, Fernando,Fukuzumi, Shunichi The Royal Society of Chemistry 2012 Physical chemistry chemical physics Vol.14 No.10

<P>Photoinduced electron-transfer dynamics of self-assembled donor–acceptor dyads formed by axial coordination of zinc naphthalocyanine, ZnNc, and perylenediimide (PDI) bearing either pyridine (py) or imidazole (im) coordinating ligands were investigated. The PDIim unit was functionalized with <I>tert</I>-octylphenoxy groups at the bay positions, which avoid aggregation providing solubility, to examine the effect of the bulky substituents at the bay positions on the rates of electron-transfer reactions. The combination between zinc naphthalocyanine and perylenediimide entities absorbs light over a wide region of the visible and near infrared (NIR) spectrum. The binding constants of the self-assembled ZnNc:PDIpy (<B>1</B>) and ZnNc:PDIim (<B>2</B>) in toluene were found to be 2.40 × 10<SUP>4</SUP> and 1.10 × 10<SUP>5</SUP> M<SUP>−1</SUP>, respectively, from the steady-state absorption and emission measurements, indicating formation of moderately stable complexes. The geometric and electronic calculations by using an <I>ab initio</I> B3LYP/6-311G method showed the majority of the highest occupied frontier molecular orbital (HOMO) on the zinc naphthalocyanine entity, while the lowest unoccupied molecular orbital (LUMO) was on the perylenediimide entities, suggesting that the charge-separated states of the supramolecular dyads are ZnNc&z.rad;<SUP>+</SUP>:PDI&z.rad;<SUP>−</SUP>. The electrochemical results suggest the exothermic charge-separation process <I>via</I> the singlet states of both ZnNc and PDI entities in nonpolar toluene. Upon coordination of perylenediimide to ZnNc, the main quenching pathway involved charge separation <I>via</I> the singlet-excited states of ZnNc and PDIs. Clear evidence of the intramolecular electron transfer from the singlet-excited state of ZnNc to PDI within the supramolecular dyads in toluene was monitored by the femtosecond laser photolysis by observing the characteristic absorption band of the PDI radical anion (PDI&z.rad;<SUP>−</SUP>) and the ZnNc radical cation (ZnNc&z.rad;<SUP>+</SUP>) in the visible and NIR regions. The rate constants of charge-separation (<I>k</I><SUB>CS</SUB>) processes of the self-assembled dyads <B>1</B> and <B>2</B> were determined to be 4.05 × 10<SUP>10</SUP> and 1.20 × 10<SUP>9</SUP> s<SUP>−1</SUP>, respectively. The rate constant of charge recombination (<I>k</I><SUB>CR</SUB>) and the lifetime of charge-separated states (<I>τ</I><SUB>CS</SUB>) of dyad <B>1</B> were determined to be 2.34 × 10<SUP>8</SUP> s<SUP>−1</SUP> and 4.30 ns, respectively. Interestingly, a slower charge recombination (2.20 × 10<SUP>7</SUP> s<SUP>−1</SUP>) and a longer lifetime of the charge separated state (45 ns) were observed in dyad <B>2</B> in nonpolar toluene by utilizing the nanosecond transient measurements. The absorption in a wide section of the solar spectrum and the high charge-separation/charge-recombination ratio suggest the usefulness of the self-assembled zinc naphthalocyanine–perylenediimide dyads as good photosynthetic models.</P> <P>Graphic Abstract</P><P>Good combination! Efficient electron transfer and relatively long-lived charge-separated states are achieved of light harvesting zinc naphthalocyanine:perylenediimide supramolecular dyads using the femtosecond transient absorption technique in nonpolar toluene. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c2cp23285e'> </P>

C₆₀ dimers connected through pleiadene bridges: fullerenes talking to each other

Sastre-Santos, Á,ngela,Parejo, Concepció,n,Martí,n-Gomis, Luis,Ohkubo, Kei,Ferná,ndez-Lá,zaro, Fernando,Fukuzumi, Shunichi Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.5

<P>New C<SUB>60</SUB> dimers 1, 2 and 3 that differ in the distance between the two balls, connected through pleiadene bridges have been synthesized. Different synthetic strategies have been used to prepare C<SUB>60</SUB> dimers 1–3. A diastereoisomeric mixture was obtained for C<SUB>60</SUB> dimer 1, which was separated by column chromatography and characterized by NMR. By CV measurements, no interaction between the two balls was found in the ground state in any type of C<SUB>60</SUB> dimer. By reduction with dimeric benzylnicotinamide [(BNA)<SUB>2</SUB>], a two electron reductant, the interaction between both C<SUB>60</SUB> units has been analyzed using electron paramagnetic resonance (EPR). For C<SUB>60</SUB> dimer 3, upon addition of 1 equivalent (BNA)<SUB>2</SUB> no electronic interaction in the C<SUB>60</SUB>˙<SUP>−</SUP>–C<SUB>60</SUB>˙<SUP>−</SUP> was observed due to the long distance between the C<SUB>60</SUB> balls (20 Å). However, for C<SUB>60</SUB> dimer 1, where the distance between radicals is shorter, 10 Å, the electronic interaction in the di(radical anion), C<SUB>60</SUB>˙<SUP>−</SUP>–C<SUB>60</SUB>˙<SUP>−</SUP> in the presence of 1 equivalent of (BNA)<SUB>2</SUB> was observed by EPR, corresponding to a triplet biradical.</P> <P>Graphic Abstract</P><P>Addition of 1 equivalent of (BNA)<SUB>2</SUB> to dimer 1 yields the C<SUB>60</SUB>˙<SUP>−</SUP>–C<SUB>60</SUB>˙<SUP>−</SUP> triplet biradical where electronic interaction is observed. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0jm02635b'> </P>

Early Effect of 0.005% Estriol Vaginal Gel on Symptoms and Signs of Vulvovaginal Atrophy

Lázaro-Carrasco de la Fuente Jesús,Cuerva González Marcos,González Rodríguez Silvia,Delgado Marín Juan Luis,Cuevas Castillo Carmen,Nieto Magro Concepción 대한폐경학회 2022 대한폐경학회지 Vol.28 No.2

Objectives: This study aims to assess the effect of ultralow dose 0.005% estriol vaginal gel in women with genitourinary syndrome of menopause (GSM).Methods: In this prospective and multicenter single-arm study, efficacy was assessed by the evaluation of the epithelial maturation value (MV), vaginal pH, symptoms and signs of vulvovaginal atrophy. Tolerability, acceptability, and the effect on intimate relationships were also evaluated.Results: We included 35 postmenopausal women with moderate-to-severe vaginal dryness. The most bothering symptom reported was vaginal dryness. The mean increase in the MV after 7 and 14 days of treatment were 22.1 (P < 0.001) and 39.9 (P < 0.001) points, with an increase in the superficial cells of 17.7 percentage points (pp) (95% confidence interval [CI], 7.9–27.4; P < 0.001) and 41.4 pp (95% CI, 28.2–54.6; P < 0.001) observed at the timepoints. Additionally, the pH decreased by 0.6 ± 0.7 (mean ± SD) at 7 days (P < 0.0001) and by 1.1 ± 0.8 at 14 days (P < 0.0001) from a baseline mean value of 6.3 ± 0.8. The severity of vaginal dryness (range, 0 [none] to 3 [severe]) was significantly reduced by a mean of 1.4 points (P < 0.0001) at 7 days and 2 points (P < 0.0001) at 14 days. Conclusions: Ultralow dose 0.005% estriol vaginal gel produced a rapid improvement of most relevant symptoms and signs of GSM. This clinically meaningful response was observed from the initial days of treatment, confirming a fast onset and a progressive action.

Volunteer Translators and Interpreters in the Field of Spanish Non-profit Organizations : An Approach to Their Profiles and Professional Identities

Raquel Lázaro Gutiérrez,Cristina Álvaro Aranda 한국외국어대학교 통번역연구소 2021 한국외국어대학교 통번역연구소 학술대회 Vol.2021 No.01

Evaluation of MgCo2O4 nanoparticles as a gas sensor for the detection of acetone in the diabetic and non-diabetic range

J. P. Morán-Lázaro,F. López-Urías,E. Muñoz-Sandoval,M. Courel-Piedrahita,A. Carreon-Alvarez,V. M. Rodríguez-Betancourtt,I. Zamudio-Torres,E. S. Guillén-López,A. Palafox-Corona 대한금속·재료학회 2023 ELECTRONIC MATERIALS LETTERS Vol.19 No.1

The acetone contained in human breath is of great interest for the health sector as it is a marker that allows to diagnoseand control diabetes in a non-invasive way. However, its concentration is extremely low. Therefore, high-performanceacetone sensors are still a challenge. With this in mind, MgCo 2 O 4 nanoparticles were synthesized using a microwaveassistedcolloidal route with subsequent calcination. Structural and morphological characterizations were done through varioustechniques. The MgCo 2 O 4 sensor was fabricated with the sample calcined at 500 °C. The sensing results showed that thesensor could detect acetone vapors ranging from 0.5 to 50 ppm at an optimum operating temperature of 250 °C with a highresponse, repeatability, stability, and selectivity. These sensing characteristics revealed that MgCo 2 O 4 could be used as a newsensor material to detect acetone in exhaled human breath.

Multidisciplinary Approach to an Extended Pressure Sore at the Lumbosacral Area

윤세훈,정의철,Hudson Alex Lázaro 대한성형외과학회 2016 Archives of Plastic Surgery Vol.43 No.6

A pressure sore wound is often extensive or complicated by local infection involving adjacent soft tissue and bone. In this case, a regional flap after simple debridement is not adequate. Here, we present a case of an extensive pressure sore in the sacral area with deep tissue infection. A 43-year-old female patient with a complicated sore with deep tissue infection had a presacral abscess, an iliopsoas abscess, and an epidural abscess in the lumbar spine. After a multidisciplinary approach performed in stages, the infection had subsided and removal of the devitalized tissue was possible. The large soft tissue defect with significant depth was reconstructed with a free latissimus dorsi musculocutaneous flap, which was expected to act as a local barrier from vertical infection and provide tensionless skin coverage upon hip flexion. The extensive sacral sore was treated effectively without complication, and the deep tissue infection completely resolved. There was no evidence of donor site morbidity, and wheelchair ambulation was possible by a month after surgery.