SMYD3-associated pathway is involved in the anti-tumor effects of sulforaphane on gastric carcinoma cells

Qing-Qing Dong,Qiu-Tong Wang,Lei Wang,Ya-Xin Jiang,Mei-Ling Liu,Hai-Jie Hu,Yong Liu,Hao Zhou,Hong-Peng He,Tong-Cun Zhang,Xuegang Luo 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.4

Sulforaphane (SFN), a natural compound derived from cruciferous vegetables, has been proved to possess potent anti-cancer activity. SMYD3 is a histone methyltransferase which is closely related to the proliferation and migration of cancer cells. This study showed that SFN could dose-dependently induce cell cycle arrest, stimulate apoptosis, and inhibit proliferation and migration of gastric carcinoma cells. Accompanied with these anticancer effects, SMYD3 and its downstream genes, myosin regulatory light chain 9, and cysteine-rich angiogenic inducer 61, was downregulated by SFN. Furthermore, overexpression of SMYD3 via transfection could abolish the effects of SFN, suggesting that SMYD3 might be an important mediator of SFN. To the best of our knowledge, this is the first report describing the role of SMYD3 in the anti-cancer of SFN. These findings might throw light on the development of novel anti-cancer drugs and functional food using SFN-rich cruciferous vegetables.

Hemocyte Changes after the Extirpation of the Hemopoietic Organ-wing Disc Complexes in the Silkworm, Bombyx mori (Lepidoptera: Bombycidae)

( Qing Xiang Zhou ),( Xing Jia Shen ),( Yong Zhu Yi ),( Ai Hua Xia ),( Zhi Fang Zhang ) 한국잠사학회 2006 International Journal of Industrial Entomology Vol.13 No.2

We successfully extirpated all four hemopoietic organ-wing disc complexes of the fifth instar larvae of Bombyx mori, and found that most of the treated silkworms could still develop into the moths. We investigated the changes of the circulating hemocytes and evaluated the effects of extirpation on the hemopoiesis. The results showed that proliferation of circulating hemocytes was sufficient to allow development of the silkworms which complexes were totally extirpated. We also found that hemopoitic organ-wing disc complexes extirpation might cause a certain hemopoietic compensation of the remainder complexes during early spinning. Exogenous hormones such as 20-β-hydrox-yecdysone and juvenile hormone analog had a positive effect on hemocytes proliferation.

Comparative Phylogenetic Relationships and Genetic Structure of the Caterpillar Fungus Ophiocordyceps sinensis and Its Host Insects Inferred from Multiple Gene Sequences

Qing-Mei Quan,Qing-Xia Wang,Xue-Li Zhou,Shan Li,Xiao-Ling Yang,Yun-Guo Zhu,Zhou Cheng 한국미생물학회 2014 The journal of microbiology Vol.52 No.2

Ophiocordyceps sinensis (Ascomycota: Ophiocordycipitaceae)is a native fungal parasite of Hepialidae caterpillars and oneof the most economically important medicinal caterpillarfungi in China. However, little is known about the phylogeneticand evolutionary relationships between O. sinensis andits host insects. In this study, nuclear ITS and β-tubulin sequencesfrom O. sinensis and mitochondrial COI, COII, andCytb sequences from its hosts were analyzed across 33 populationssampled from five regions in China. Phylogenetically,both O. sinensis and its hosts were divided into three geographicallycorrelated clades, and their phylogenies were congruent. Analysis of molecular variance and calculated coefficientsof genetic differentiation revealed significant geneticdivergence among the clades within both O. sinensis (FST=0.878, NST=0.842) and its hosts (FST=0.861, NST=0.816). Estimatedgene flow was very low for O. sinensis (Nm=0.04) andthe host insects (Nm=0.04) among these three clades. Manteltests demonstrated a significant correlation (P<0.01) betweenthe genetic distances for O. sinensis and its hosts, as well as asignificant association (P<0.05) between geographic and geneticdistances in both. The similar phylogenetic relationships,geographic distributions, and genetic structure and differentiationbetween O. sinensis and its hosts imply that they have coevolved.

A Study on the Development Strategy of China’s Aquatic Products Export to South Korea

Zhou Qing 한중경제문화학회 2023 한중경제문화연구 Vol.24 No.-

Aquatic products have been exported and exported in large quantities as they provide high-quality protein, promote brain development and maintain cardiovascular health. China’s exports of aquatic products will reach 21. 926billion U. S. dollars in 2021, up 15. 31 percent year-on-year and 23. 03 billion U. S. dollars in 2022, an increase of 5. 04 percent year-on year. Based on this background, this paper will analyze the export structure of Chinese fisheries products based on HS code and analyze the competitiveness of Chinese fisheries products through trade competition index (TC). The paper points out the existing problems in China’s export of fishery products to Korea and puts forward a targeted and effective development strategy.

Improved Method for Calculating Magnetic Field of Surface-Mounted Permanent Magnet Machines Accounting for Slots and Eccentric Magnet Pole

Zhou, Yu,Li, Huaishu,Wang, Wei,Cao, Qing,Zhou, Shi The Korean Institute of Electrical Engineers 2015 Journal of Electrical Engineering & Technology Vol.10 No.3

This paper presented an improved analytical method for calculating the open-circuit magnetic field in the surface-mounted permanent magnet machines accounting for slots and eccentric magnet pole. Magnetic field produced by radial and parallel permanent magnet is equivalent to that produced by surface current according to equivalent surface-current method of permanent magnet. The model is divided into two types of subdomains. The field solution of each subdomain is obtained by applying the interface and boundary conditions. The magnet field produced by equivalent surface current is superposed according to superposition principle of vector potential. The investigation shows harmonic contents of radial flux density can be reduced a lot by changing eccentric distance of eccentric magnet poles compared with conventional surface-mounted permanent-magnet machines with concentric magnet poles. The FE(finite element) results confirm the validity of the analytical results with the proposed model.

Apoptotic Effects of psiRNA-STAT3 on 4T1 Breast Cancer Cells in Vitro

Zhou, Yue,Tian, Lin,Zhang, Ying-Chao,Guo, Bao-Feng,Zhou, Qing-Wei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.16

Background: The aim of this study was to investigate the effect of a Lipofectamine2000 (Life2000) Transfection Reagent transfected psiRNA-STAT3 plasmid on 4T1 breast cancer cells. Materials and Methods: MTT was used to detect the cell proliferation of breast cancer 4T1 cells at different periods (0h, 6h, 8h, 10h); the cell cycle was assessed by flow cytometry; variation of apoptosis and mitochondrial membrane potential was observed under a fluorescence microscope; immunohistochemical staining was used to determine the expression of caspase-3 and cyclin-D1 protein. Results: An obvious effect of inhibition to 4T1 cancer cells could be observed at 8h after the psiRNA-STAT3 was transfected. Typical alterations of apoptotic morphological features were visible in the psiRNA-STAT3 treatment group. Mitochondrial membrane potential decreased significantly, the number of cells was increased in G0/G1 phase, and the number of cells was decreased in S phase, and the data were statistically significant (p<0.05), compared with the Scramble and Mock groups. Expression of caspase-3 protein was increased significantly, while that of cyclin D1 was significantly decreased. Conclusions: Life2000 transfected psiRNA-STAT3 plasmid can inhibit 4T1 tumor cell proliferation and promote apoptosis of 4T1 tumor cells, which process depends on the regulation of expression of cyclin D1 and caspase-3 protein.

Original Article: Food Science/Microbiology : Antioxidant and Antimicrobial Activities of Camellia Oleifera Seed Oils

( Qing Fen Zhou ),( Xue Jing Jia ),( Qian Qian Li ),( Rui Wu Yang ),( Li Zhang ),( Yong Hong Zhou ),( Chun Bang Ding ) 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.2

The antioxidant and antimicrobial activities of Camellia oleifera seed oil were studied. Four kinds of seed oil samples were prepared, crude oil and refined oil, extracted by cold pressing method (CPC, CPR), and organic solvent extraction (OSC, OSR). Antioxidant activity analysis was measured in 2,2-azinobis (3- ethylbenzothiazoline-6-sulfonic acid)-diammonium salt, ferric reducing Ability of Plasma, and 2,2-diphenyl-1-picrylhydrazyl assays. Besides, the percentage of inhibition of red blood cells hemolysis induced by 2,2´-azobis(2-amidnopropane) dihydrochlorid, the lag time of LDL conjugated dienes formation in vitro, and the inhibitors of loss in tryptophan fluorescence were all used to estimate the antioxidant activity of the samples. The total phenolic contents (TPC) were detemined by Folin-Ciocalteu method. The TPC of the C. oleifera seed oils can be arranged in descending order: CPC (1.9172 μg/mL) > OSC (1.5218 μg/mL) > CPR (1.0611 μg/mL) > OSR (0.6782 μg/mL). And the oils were investigated for activity against Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger. The results showed the antioxidant activity of crude oil by cold pressing method was stronger than others, and all oils did inhibit activity of the top three bacteria expert A. niger. The further significance of the study contributes to measure the antioxidant and antimicrobial activity of the potential health benefits by the different methods of preparation and the oil of C. oleifera seeds acting as free radical scavenger, pharmaceuticals and preservatives may offer some information in medicine and cosmetic not just in food field.

L^p-SOLUTIONS FOR REFLECTED BSDES WITH TIME DELAYED GENERATORS

Zhou, Qing Korean Mathematical Society 2016 대한수학회보 Vol.53 No.3

In this paper, we establish the existence and uniqueness of the solution for a class of reflected backward stochastic differential equations with time delayed generator (RBSDEs with time delayed generator, in short) in the case when the terminal value and the obstacle process are $L^p$-integrable with p ${\in}$]1, 2[ for a sufficiently small Lipschitz constant of the generator and the time horizon T.

Metabolomics Investigation of Cutaneous T Cell Lymphoma Based on UHPLC-QTOF/MS

Zhou, Qing-Yuan,Wang, Yue-Lin,Li, Xia,Shen, Xiao-Yan,Li, Ke-Jia,Zheng, Jie,Yu, Yun-Qiu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Objectives: The identification of cutaneous T cell lymphoma (CTCL) biomarkers may serve as a predictor of disease progression and treatment response. The aim of this study was to map potential biomarkers in CTCL plasma. Design and Methods: Plasma metabolic perturbations between CTCL cases and healthy individuals were investigated using metabolomics and ultrahigh performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). Results: Principal component analysis (PCA) of the spectra showed clear metabolic changes between the two groups. Thirty six potential biomarkers associated with CTCL were found. Conclusions: Based on PCA, several biomarkers were determined and further identified by LC/MS/MS analysis. All of these could be potential early markers of CTCL. In addition, we established that heparin as a nticoagulant has better pre-treatment results than EDTA with the UHPLC-QTOF/MS appraoch.

Overview of aristolochic acid nephropathy: an update

Zhou Qing-Qing,Jiang Lei,Tao Su,Gang Liu,Yang Liu 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.5

Aristolochic acid nephropathy (AAN) is a rapidly progressive renal interstitial fibrosis caused by medical or environmental exposure to aristolochic acid (AA). Since the outbreak of AAN in Belgium was reported nearly 30 years ago, the safety of herbal remedies has drawn considerable attention, and AAN has become a global public health problem. Breakthroughs have been made to better understand the disease, including the toxicity of AAs, the possible mechanisms of AAN, the disease patterns, and the pathological features; however, some critical problems remain unresolved. Because of the insidious onset of the disease, the incidence of AAN and the prevalence of exposure to AAs are unknown and might be largely underestimated. During the past decades, AA-containing herbs have been strictly administrated in many regions and the occurrence of AAN has declined sharply, yet cases of AAN are still sporadically reported. Despite the progress in the understanding of the disease’s pathogenesis, there is no effective treatment for delaying or reversing the renal deterioration caused by AAN. Therefore, the risk of exposure to AAs should be taken seriously by public health workers and clinicians. In this review, we updated the latest data on AAN, summarized the advances throughout these years, and put forward some challenges for future research.