Carbon Paper-Supported NiCo/C–N Catalysts Synthesized by Directly Pyrolyzing NiCo-Doped Polyaniline for Oxygen Reduction Reaction

Zhongliang Deng,Qingfeng Yi,Yuanyuan Zhang,Huidong Nie,Guang Li,Liang Yu,Xiulin Zhou 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.1

In this study, we report the findings that the C–N composites containing Ni and Co (Ni1Co1/C–N, Ni3Co1/C–N, Ni6Co1/C–N, Ni9Co1/C–N, Ni10Co0/C–N and Ni0Co10/C–N) can be produced by direct pyrolysis of the NiCo-doped polyaniline (PANI) precursors in N2 atmosphere at 800 ℃ and show efficient electroactivity for oxygen reduction reaction (ORR) in alkaline media. Distribution and compositions of the catalysts were characterized by SEM, TEM, EDS and XRD techniques. The catalysts were loaded on carbon paper to prepare gas diffusion electrodes, in which electrocatalytic activity for ORR in alkaline media was investigated by voltammetric techniques. The ORR current density on these carbon paper-supported NiCo/C–N catalysts exhibits a linear increase with the negative shift of ORR potential. The ORR onset potential is around 0.2 V (versus Ag/AgCl) in alkaline media. Among the prepared catalysts, the catalyst Ni6Co1/C–N presents the largest ORR current density, which is 68.5 mA cm -2@-0.8 V (versus Ag/AgCl) in alkaline media. Moreover, Ni6Co1/C–N catalyst also presents good electrocatalytic activity stability for ORR.

Blood–brain barrier disruption caused by neonatal sevofluraneinduced depends on exposure time and is reversible in mice

Zhongliang Sun,Maiko Satomoto,Yushi U Adachi,Koshi Makita 대한마취통증의학회 2019 Korean Journal of Anesthesiology Vol.72 No.4

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Apocynin preserves glutamatergic neurons in the basolateral amygdala in mice with neonatal sevoflurane exposure

ZhongLiang Sun,Maiko Satomoto,Yushi U Adachi,Koshi Makita 대한마취통증의학회 2017 Korean Journal of Anesthesiology Vol.70 No.3

Background: Neonatal exposure to anesthetics induces neuronal apoptosis and long-term cognitive dysfunction in rodents. We showed that the nicotinamide adenine dinucleotide phosphate-oxidase inhibitor apocynin not only reduces neurotoxicity by decreasing superoxide levels and preventing mitochondrial dysfunction but also improves long-term memory impairment in neonatal mice exposed to sevoflurane. We also found that after the contextual fear conditioning test, glutamatergic neurons expressed c-Fos (neural activation) regardless of previous exposure to sevoflurane. Moreover, there were fewer c-Fos-expressing glutamatergic neurons in the basolateral amygdala (BLA) after exposure to sevoflurane than after exposure to carrier gas. In this study, we investigated whether the administration of apocynin prior to sevoflurane exposure would preserve glutamatergic neurons in the BLA. Methods: Apocynin (50 mg/kg) was injected intraperitoneally into six-day-old male mice 30 min before 6 h of exposure to 3% sevoflurane or carrier gas only. The mice were allowed to mature and then were subjected to the contextual fear conditioning test. The neural activation and neuron population in the BLA were investigated 2 h later. Results: Administration of apocynin prior to neonatal sevoflurane exposure not only prevented learning deficits but also preserved c-Fos-expressing glutamatergic neurons in the BLA. Conclusions: Apocynin mitigates the cognitive impairment induced by neonatal sevoflurane exposure and preserves c- Fos-expressing glutamatergic neurons in the basolateral amygdala.

Identification and comparative analysis of the pearl oyster Pinctada fucata hemocytes microRNAs in response to Vibrio alginolyticus infection

Zhongliang Wang,Bei Wang,Gang Chen,Yishan Lu,Jichang Jian,Zaohe Wu 한국유전학회 2017 Genes & Genomics Vol.39 No.10

MicroRNAs (miRNAs) are a class of noncoding RNA molecules that function as negative regulators of gene expression and play important roles in a wide spectrum of biological processes, including in immune response. However, the physiological regulation function of Pinctada fucata miRNAs, specially their immunomodulation has not been explored yet. Here, two small RNA libraries from hemocytes of P. fucata with or without Vibrio alginolyticus infection were constructed and sequenced using the high-throughput Illumina deep sequencing technology. In total, 11,939,992 and 11,083,327 raw reads, corresponding to 10,993,546 and 9,988,179 clean reads, were respectively obtained in the control and infected libraries. A total of 276 miRNAs, including 225 known miRNAs and 51 putative novel miRNAs, were identified by bioinformatic analysis. By using pairwise comparison between two libraries, 93 miRNAs were found to be significantly differentially expressed, with 42 and 51 miRNAs exhibiting up-regulation and down-regulation, respectively. Thereinto, some known miRNAs were considered to be immune-related. Real-time PCR were implemented for 6 miRNAs co-expressed in the control and infected samples, and agreement was confirmed between the high-throughput sequencing and real-time PCR data. After miRNA targets were predicted, GO and KEGG pathway enrichment analysis were performed, and the results indicated that ten of the differentially expressed miRNAs were involved in immunerelated pathways, and might participate in the host immune response to V. alginolyticus. These results of identification and comparative analysis of miRNAs might deepen our understanding of host-pathogen interactions and immune defense mechanisms in P. fucata.

Fabrication and application of silicalite-1 and TS-1 hollow fibers with polyethylene imine (PEI) fibers as substrates

Zhongliang Sun,Yongming Zhang,Xiangyun Deng,Jianbao Li,Chunrong Xiong 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.1

Silicalite-1 and titanium containing silicalite-1 (TS-1) hollow fibers were fabricated with polyethylene imine (PEI) fibers as substrates. The acid treated PEI fibers were positively charged and can be effectively coated with silicalite-1 or TS-1 nanocrystals from their colloidal solution. The adsorbed silicalite-1 or TS-1 nanocrystals grew up and became more compact upon two days of vapor phase Ostwald ripening in autoclave. Silicalite-1 and TS-1 hollow fibers were obtained after calcination at 400 8C in air. The shell thickness of the hollow fibers is 1 mm. Hydroxylation of phenol with H2O2 was investigated over TS-1based catalysts in order to compare influences of nanocrystals composed macrostructure and Al2O3binder on the catalytic activities.

The Bovine Iris Location Method Based on Dynamic Contour Tracking and Least Square Principle

Zhongliang Luo,Yingbiao Jia 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.7

Bovine iris recognition technology plays a very important role in meat food traceability system about large-scale livestock individual identification. In order to improve the precision and speed of bovine iris location, iris inner and outer edge location method based on dynamic contour tracking and least square principle ellipse fitting were respectively proposed in the paper, according to the characteristics of bovine iris image. For iris inner edge location, firstly, the pupil centroid was determined, and pupil sub-image was separated from eye image, then, using dynamic contour tracking method based on the non-initialized level set model to track the boundary of pupil, and got iris inner boundary. For iris outer edge location, noise reducing template was determined by pupil sub-image, which combined with mathematical morphology operators to further suppress random noise, so, ideal outer edge detection curve was obtained. Finally, it got iris outer edge of ellipse fitting based on least squares principle. Experimental results shown that this method was less running time, high location accuracy, it has a certain practicality in large-scale livestock meat food traceability.

Therapeutic effects of intravenous administration of bone marrow stromal cells on sevofluraneinduced neuronal apoptosis and neuroinflammation in neonatal rats

ZhongLiang Sun,Maiko Satomoto,Koshi Makita 대한마취통증의학회 2015 Korean Journal of Anesthesiology Vol.68 No.4

Background: Sevoflurane exposure during the early postnatal period causes neuroinflammation and neuronal apoptosis in rodents. Bone marrow stromal cells (BMSCs) have been shown to protect and repair the damaged central nervous system, for example in ischemic stroke models. In this study, we investigated whether intravenous administration of BMSCs ameliorated neurodegeneration, induced by sevoflurane exposure, in neonatal rats. Methods: Sprague-Dawley rat pups (postnatal day 7) were exposed to 2% sevoflurane for 6 h (vehicle group, n = 7). BMSCs were administered 30 min after induction of sevoflurane anesthesia (BMSCs group, n = 7). The pups were exposed to carrier gas only, as a negative control (mock anesthesia group, n = 4). We assessed the therapeutic effects of BMSC treatment by measuring expression of the pro-inflammatory cytokine interleukin-6 (IL-6), and levels of cleaved caspase-3, in brain tissues immediately following sevoflurane anesthesia. Results: Analysis of the cleaved caspase-3 bands revealed that levels of activated caspase-3 were elevated in the vehicle group compared with the mock anesthesia group, indicating that a single exposure to sevoflurane at subclinical concentrations can precipitate neuronal apoptosis. BMSC treatment did not suppress apoptosis induced by sevoflurane exposure (compared with the vehicle group). The vehicle group had higher proinflammatory cytokine IL-6 protein levels compared with the mock anesthesia group, indicating that sevoflurane exposure induces IL-6 expression. BMSC treatment suppressed sevoflurane-induced increases in IL-6 expression, indicating that these cells can inhibit the neuroinflammation induced by sevoflurane exposure (vehicle group vs. BMSC group). Conclusions: Intravenous administration of BMSCs reduces neuroinflammation, but does not attenuate apoptosis induced by sevoflurane exposure.

Expression of VEGF-C/VEGFR-3 in Human Laryngeal Squamous Cell Carcinomas and its Significance for Lymphatic Metastasis

Wang, Zhongliang,Chen, Yao,Li, Xiaofeng,Xu, Li,Ma, Wei,Chang, Lingmei,Ju, Funian Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.1

Objectives: Expression of vascular endothelial growth factor C (VEGF-C)and vascular endothelial growth factor feceptor-3 (VEGFR-3) in laryngeal squamous carcinoma and its relationship to lymph node metastasis were investigated. Methods: VEGF-C and VEGFR-3 gene expression in 30 cases of normal laryngeal mucosa tissue (NLM), primary laryngeal carcinoma cell carcinomas (PLC) and cervical lymph nodes (CLN) was examined by reverse transcription polymerase chain reaction (RT-PCR). Protein levels of VEGF-C expression were determined by immunohistochemical staining in 60 cases of PLC. Results: Expression of VEGF-C and VEGFR-3 different among NLM, PLC and CLN in the same patient. In PLC, expression was significantly higher in lymph node positive group than in the lymph node negative group and associated with histological grade of differentiation; Expression of VEGF-C and VEGFR-3 was not linked with age, sex, site or T stage. Conclusions: A close correlation was found between VEGF-C/VEGFR-3 expression and lymph node metastasis in PLC, suggesting a role in metastasis of laryngeal carcinomas.

Regulation of chicken vanin1 gene expression by peroxisome proliferators activated receptor α and miRNA-181a-5p

Wang, Zhongliang,Yu, Jianfeng,Hua, Nan,Li, Jie,Xu, Lu,Yao, Wen,Gu, Zhiliang Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.2

Objective: Vanin1 (VNN1) is a pantetheinase that can catalyze the hydrolysis of pantetheine to produce pantothenic acid and cysteamine. Our previous studies showed that VNN1 is specifically expressed in chicken liver. In this study, we aimed to investigate the roles of peroxisome proliferators activated receptor α (PPARα) and miRNA-181a-5p in regulating VNN1 gene expression in chicken liver. Methods: 5'-RACE was performed to identify the transcription start site of chicken VNN1. JASPAR and TFSEARCH were used to analyze the potential transcription factor binding sites in the promoter region of chicken VNN1 and miRanda was used to search miRNA binding sites in 3' untranslated region (3'UTR) of chicken VNN1. We used a knock-down strategy to manipulate PPARα (or miRNA-181a-5p) expression levels in vitro to further investigate its effect on VNN1 gene transcription. Luciferase reporter assays were used to explore the specific regions of VNN1 targeted by PPARα and miRNA-181a-5p. Results: Sequence analysis of the VNN1 promoter region revealed several transcription factor-binding sites, including hepatocyte nuclear factor 1α (HNF1α), PPARα, and CCAAT/enhancer binding protein α. GW7647 (a specific agonist of PPARα) increased the expression level of VNN1 mRNA in chicken primary hepatocytes, whereas knockdown of PPARα with siRNA increased VNN1 mRNA expression. Moreover, the predicted PPARα-binding site was confirmed to be necessary for PPARα regulation of VNN1 gene expression. In addition, the VNN1 3'UTR contains a sequence that is completely complementary to nucleotides 1 to 7 of miRNA-181a-5p. Overexpression of miR-181a-5p significantly decreased the expression level of VNN1 mRNA. Conclusion: This study demonstrates that PPARα is an important transcriptional activator of VNN1 gene expression and that miRNA-181a-5p acts as a negative regulator of VNN1 expression in chicken hepatocytes.

Testing a Hypothesis of Fault Plane Determination Based on CMT Centroid Locations

Wu, Zhongliang,Kim, So Gu 한국지진연구소 1996 SAFE Vol.2 No.1

It was proposed that the hypocenter location reflects the initiation of the earthquake rupture, while the CMT centroid reflects the centroid of the moment release. In this note such a hypothesis is tested by analyzing the recent CMT data. It is assumed that if the hypothesis were acceptable, the vector from the hypocenter location to the centroid location would lie in one of the nodal planes of the focal mechanism, and the rupture would propagate along the hypocenter-to-centroid vector with the apparent rupture velocity being of the same order as the S-wave velocity or less, The CMT results from May 1992 to February 1994 are analyzed. The results show that the hypothesis seems acceptable in an overall sense. Furthermore, for shallow earthquakes, it may be possible to deduce the preferred nodal plane by comparing the hypocenter location and the centroid location.