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      • SCIESCOPUSKCI등재

        Immunization of BALB/c mice with <i>Brucella abortus </i>2308Δ<i>wbkA</i> confers protection against wild-type infection

        Zhi-qiang,Li,Dan,Gui,Zhi-hua,Sun,Jun-bo,Zhang,Wen-zhi,Zhang,Hui,Zhang,Fei,Guo,Chuang-fu,Chen 대한수의학회 2015 JOURNAL OF VETERINARY SCIENCE Vol.16 No.4

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 7시까지 원문보기가 가능합니다.

        Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the <i>Brucella (B.) abortus</i> 2380 <i>wbkA</i> mutant (2308Δ<i>wbkA</i>) and evaluated its virulence. The survival of 2308Δ<i>wbkA</i> was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The <i>wbkA </i>mutant elicited an anti-<i>Brucella</i>-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308Δ<i>wbkA</i> could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The <i>WbkA </i>antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308Δ<i>wbkA</i> is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.

      • KCI등재SCOPUS

        Myopericytoma Involving the Parotid Gland as Depicted on Multidetector CT

        Zhi-Gang,Chu,Jian-Qun,Yu,Zhi-Gang,Yang,Zhi-Yu,Zhu,Hong-Mei,Yuan 대한영상의학회 2009 Korean Journal of Radiology Vol.10 No.4

        Myopericytoma is a newly proposed subgroup of perivascular tumors in the World Health Organization classification of soft tissue tumors. In this study, we report a case of a benign myopericytoma with detailed multidetector CT (MDCT) findings in the parotid gland, a location that has not been described for this type of tumor previously. The clinical presentation, imaging features, histopathological and immunohistochemical findings, and the differential diagnosis with other tumors in the parotid gland are described and reviewed.

      • KCI등재SCOPUS

        Immunization of BALB/c mice with Brucella abortus 2308DwbkA confers protection against wild-type infection

        Zhi-qiang,Li,Dan,Gui,Zhi-hua,Sun,Jun-bo,Zhang,Wen-zhi,Zhang,Hui,Zhang,Fei,Guo,Chuangfu,Chen 대한수의학회 2015 JOURNAL OF VETERINARY SCIENCE Vol.16 No.4

        Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308DwbkA) and evaluated its virulence. The survival of 2308DwbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308DwbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308DwbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.

      • RALY RNA Binding Protein-like Reduced Expression is Associated with Poor Prognosis in Clear Cell Renal Cell Carcinoma

        Cui,,Zhi-Wen,Xia,,Ye,Ye,,Yi-Wang,Jiang,,Zhi-Mao,Wang,,Ya-Dong,Wu,,Jian-Ting,Sun,,Liang,Zhao,,Jun,Fa,,Ping-Ping,Sun,,Xiao-Juan,Gui,,Yao-Ting,Cai,,Zhi-Ming Asian Pacific Organization for Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

        The molecular mechanisms involved in the progression of clear cell renal cell carcinomas (ccRCCs) are still unclear. The aim of this study was to analyse the relationships between expression of RALYL and clinical characteristics. In 41 paired samples of ccRCCs and adjacent normal tissues, we used real-time qPCR to evaluate the expression of RALYL mRNA. RALYL protein levels were determined in 146 samples of ccRCC and 37 adjacent normal tissues by immunohistochemistry. Statistical analysis was used to explore the relationships between expression of RALYL and the clinical characteristics (gender, age, tumor size, T stage, N stage, M stage, survival times and survival outcome) in ccRCC. In addition, these patients were follow-up period 64 months (range: 4~116months) to investigate the influence on prognosis. We found significantly differences between ccRCC tissues and normal tissues (p<0.001, paired-sample t test) in mRNA levels of RALYL. Immunohistochemistry analyses in 146 ccRCC samples and 37 adjacent normal tissues showed significantly lower RALYL protein levels in ccRCC samples (${\chi}^2$-test, p<0.001), inversely correlating with tumour size (p=0.024), T stage (0.005), N stage (p<0.001) as well as M stage (p=0.019), but not age (p=0.357) and gender (p=0.348). Kaplan-Meier survival analysis demonstrated that people with lower level of RALYL expression had a poorer survival rate than those with a higher level of RALYL expression, significantly different by the log-rank test (p=0.011). Cox regression analysis indicated that RALYL expression (p=0.039), N stage (p=0.008) and distant metastasis (p<0.001) were independent prognosis factors for the overall survival of ccRCC patients. We demonstrated that the expression of RALYL was significantly low in ccRCC and correlated with a poor prognosis in a large number of clinical samples. Our findings showed that RALYL may be a potential therapeutic target as well as a poor prognostic factor.

      • Comparison and Analysis of Street Tree in Beijing, China, and Daejeon, South Korea : Mainly focused on species diversity

        Li,Zhi,Hua,,Seo,Byung,Key,,Li,Zhi,Hui 배재대학교 자연과학연구소 2011 自然科學論文集 Vol.22 No.1

        중국 북경과 한국 대전의 가로수 비교 분석을 통해서 가로수의 다양성과 생태적, 미적 그리고 사회기능성을 높이기 위한 기초자료를 제공하고자 문헌조사와 현지답사를 통해 수행한 연구결과는 다음과 같다. 두 도시의 공통점은 가로수의 종 다양성이 낮으면서도 일부 수종이 큰 비율을 차지하고 있다는 점과, 상록수의 비율이 매우 낮았다는 점, 그리고 대전의 일부 가로수 구간에서 지피식물과 혼식하여 좀 더 풍부한 경관을 창출한 것을 제외하고는, 가로수의 배치 형태 또한 두 도시 모두 단순한 열식형태라는 점과 가로수 수령이 높지 않다는 점을 들 수 있다. 차이점으로는 북경 가로수는 외래종의 비율이 6.3%로 낮은 반면 대전 가로수는 외래종의 비율이 66.1%로 월등히 높아서 대전의 지역특성을 나타내지 못하였다는 점이 가장 컸다. 하지만 북경 가로수의 관리 상태는 대전의 가로수에 비해 양호한 편은 아닌 것으로 보였다. 한편, 화목류, 단풍이 예쁘게 드는 수종, 열매를 볼 수 있는 수종 등은 색감이 좋을 뿐만 아니라 계절감을 제공해 주므로 두 도시 모두 더 많이 도입할 필요가 있을 것으로 판단되었다.

      • KCI등재SCIESCOPUS

        Simultaneous Information and Power Transfer for Multi-antenna Primary-Secondary Cooperation in Cognitive Radio Networks

        Zhi,Hui,Liu,Wen,Jun,Xu,Sheng,Yu,Li,Cheng,Zhi,Long,Jia,Ru,Lin 한국전자통신연구원 2016 ETRI Journal Vol.38 No.5

        In this paper, cognitive radio and simultaneous wireless information and power transfer (SWIPT) are effectively combined to design a spectrum-efficient and energy-efficient transmission paradigm. Specifically, a novel SWIPT-based primary-secondary cooperation model is proposed to increase the transmission rate of energy/spectrum constrained users. In the proposed model, a multi-antenna secondary user conducts simultaneous energy harvesting and information forwarding by means of power splitting (PS), and tries to maximize its own transmission rate under the premise of successfully assisting the data delivery of the primary user. After the problem formulation, joint power splitting and beamforming optimization algorithms for decode-and-forward and amplify-and-forward modes are presented, in which we obtain the optimal PS factor and beamforming vectors using a golden search method and dual methods. Simulation results show that the proposed SWIPTbased primary-secondary cooperation schemes can obtain a much higher level of performance than that of non-SWIPT cooperation and non-cooperation schemes.

      • KCI등재SCOPUS

        Fault Analysis and Tolerant Control for High Speed PEMS Maglev Train End Joint Structure with Disturbance Rejection

        Zhi-Qiang,Wang,Zhi-Qiang,Long,Xiao-Long,Li 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.3

        It is observed in test operation that for permanent magnet electro-magnetic suspension (PEMS) type high speed maglev train, when a single levitation unit in an end joint structure fails to work, the performance of the entire joint structure deteriorates rapidly, whereas in electro-magnetic suspension (EMS) type maglev system, there is no such phenomenon under the same failure. To solve this problem and to enhance system performance under faulty condition, fi rstly a comparative modeling of EMS and PEMS maglev end joint structure system was made to analyze the underlying reasons. Then a dynamic compensation method and a disturbance rejection method based on disturbance estimation and feedback linearization were proposed respectively. The performances of the proposed measures are verifi ed and compared in simulations.

      • KCI등재SCOPUS

        The horizontal stability of an FLNG with different turret locations

        Zhi-Tian,Xie,Jian-Min,Yang,Zhi-Qiang,Hu,Wen-Hua,Zhao,Jing-Rui,Zhao 대한조선학회 2015 International Journal of Naval Architecture and Oc Vol.7 No.2

        The horizontal stability of a Single-Point Moored (SPM) Floating Liquefied Natural Gas (FLNG) facility is investigated. Both numerical and experimental studies have been conducted for this SPM FLNG. The numerical simulations feature well the experimental data. The effects of the turret locations are studied based on the validated numerical model. Statistic results of the vessel's motions with different turret locations are conducted and compared. The results show that the longitudinal location of the turret has a significant influence on the horizontal stability, which has a strong relationship with the yaw and roll motions. The calculated top tensions on the hawsers also develop a regular change as changing the turret's location. The investigation will provide a brief of principles with more details for the design of the ongoing project.

      • KCI등재

        Molecular Characterization of Ca_(∨)2.3 in Rat Trigemical Ganglion Neurons

        Zhi,Fang,Kim,,Joong-Soo,Oh,,Seog-Bae| The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

        R-type(Ca_(∨)2.3) calcium channel contributes to pain sensation in peripheral sensory neurons. Six isoforms of Ca_(∨)2.3 that result from combinations of presence or delection of three inserts(inserts Ⅰ and insert in the Ⅱ-Ⅲ loop, and insert Ⅲ in N-terminal regions) have been demonstrated to be present in different mammalian tissues. However, the molecular basis of Ca_(∨)2.3 in trigeminal ganglion(TG) neurons is not known. In the present study, we determined which isoforms of Ca_(∨)2.3 are expressed in rat TG neurons using the RT-PCR analysis. Whole tissue RT-PCR analyses revealed that only two isoforms, Ca_(∨)2.3a and Ca_(∨)2.3e, were present in TG neurons. From single-cell RT-PCR, we found that Ca_(∨)2.3e rather than Ca_(∨)2.3a was the major isoform expressed in TG neurons, and Ca_(∨)2.3e was preferentially detected in small-sized neurons that express nociceptive marker, transient receptor potential vanilloid 1(TRPV1). Our results suggest that Ca_(∨)2.3e in trigeminal neurons may be a potential target for the pain treatment.

      • KCI등재

        Transcriptome profile of Chinese bush cricket, Gampsocleis gratiosa: A resource for microsatellite marker development

        Zhi‐Jun,Zhou,Xiao-Yan,KOU,Lei-YangQIAN,Jing,LIU 한국곤충학회 2016 Entomological Research Vol.46 No.3

        The Chinese bush cricket, Gampsocleis gratiosa, has a long history as a pet in China. To date, the sequencing of its whole genome is unavailable as a non‐model organism. Transcriptomic information is also scarce for this species. The G. gratiosa transcriptome was sequenced using Illumina HiSeq 2000 paired‐end sequencing technology. In total 52 million clean reads with an average length of 90 bp were generated, which produced 74,821 unigenes with a mean length of 580 bp and an N50 length of 759 bp. In total 29,674 (39.66%) unigenes were successfully annotated against the NR, NT, Swiss‐Prot, Gene Ontology (GO), Clusters of Orthologous Groups of proteins (COGs) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. Further functionally classified of unigenes against GO, COGs and KEGG found that a total of 11,935 (19.95%) unigenes were categorized into 61 GO terms, 19,576 unigenes were clustered into 25 COG functional categories and 17,971 unigenes were assigned to 258 KEGG pathways. In addition, 2093 microsatellite loci were identified, of which 591 loci had flanking sequences suitable for polymerase chain reaction (PCR) primer design. The transcriptome profile of G. gratiosa contributed to the accumulation of orthopteran genomic data, and the microsatellite loci provided useful tools for future studies of this and other closely related Gampsocleis species.

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