韩国汉语学习者的礼貌用语教学研究

张蕊 ( Zhang¸ Rui ) 중국어문학회 2021 中國語文學誌 Vol.- No.74

Chinese and Korean have differences in traditional culture, way of thinking, psychological quality and customs due to their different nationalities. These differences are also reflected in the polite expressions that reflect social etiquette norms. This paper first combed the definition of Chinese politeness and politeness principles, and analyzed the differences and similarities between Chinese and Korean in politeness terms through specific examples. Secondly, a questionnaire survey is conducted among Korean undergraduates who have different degrees of Chinese learning experience. It analyzed the misuse and characteristics of Korean students’ Polite expressions, and understands the interference factors in the acquisition of polite expressions “Depreciate yourself and respect others” is one of the five principles of polite language in Chinese, and it is also the center of the five principles, and is an important connotation of Chinese culture. Chinese polite language requires high vocabulary changes. Different pragmatic phenomena will produce different polite effects. In the choice of wording, full consideration should be given to the identity, age, and occasion of both parties in the conversation. On the one hand, Korean uses a respectful vocabulary system to highlight the speaker’s respect for the other party. On the other hand, Korean has a very strict honorific style, which expresses respect through a grammatical system. At the end of the thesis, three suggestions for polite language teaching were put forward, and the teaching plan was designed. First, understand the obstacles to students’ acquisition and increase the importance of polite language teaching. Second, pay attention to the understanding and application of pragmatic functions, and emphasize cultural cognition in combination with specific scenarios. Third, make up for the lack of teaching and teaching materials, and conduct polite language teaching in stages.

Study and Design of High Strength and Low Heat Generation Polymer Composites by Molecular Dynamics Simulation

Rui-Rui Zhang,Bin Wu,Peng Chen,Jia-Sheng Qian 한국고분자학회 2020 폴리머 Vol.44 No.6

The molecular dynamics simulation was used to study and design a polymer composites system with excellent mechanical strength and less heat generation in a dynamic process. A series of system factors such as filler loading, surface modification onto filler, and network of cross-linking filler particles on mechanical and heat generation of polymer composites are systematically considered. It is found that the surface grafting onto fillers can restrain the heat generation of polymer composite in the dynamic process, while it shows less effect on the mechanical property. A network of cross-linking filler particles can be fabricated by a combination of grafting chains onto fillers. By filling such a network into the polymer, the mechanical and heat generation properties of polymer composites are significantly improved. Simulation results can help experimental fabrication of polymer composites with excellent mechanical and heat generation properties.

Historical Long-term Exposure to Pentachlorophenol Causing Risk of Cancer - A Community Study

Zheng, Rui-Zhi,Zhang, Qing-He,He, Yi-Xin,Zhang, Qian,Yang, Lin-Shen,Zhang, Zhi-Hua,Zhang, Xiu-Jun,Hu, Jing-Ting,Huang, Fen Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Background: Pervious studies suggested occupational workers exposure to pentachlorophenol (PCP) might contribute to increased risk of cancer. However, few studies have focused on associations between PCP and cancer risk at the community level. Objective: The present study was to explore the cancer risk for the community population living long-term in a PCP contaminated area. Methods: All the cancer cases diagnosed in 2009-2011 in Tongling City were collected. The cancer patients' residencies were geo-referenced in each district. The historical PCP usage for each district of Tongling was calculated as the PCP pollution index, which was further used to divide into PCP exposure categories. Standardized rate ratios (SRRs) of cancer incidence were applied to detect the cancer risk as exposure grade elevated. Correlation analysis was performed to analyze the relationship between PCP pollution and cancer incidence. Results: A total of 5,288 cancer cases (3,451 male and 1,837 female) were identified. PCP usage was correlated with the incidence of leukemia (r=0.88, P=0.002) for males, and with cancer of the esophagus for males (r=0.83, P=0.008) and females (r=0.71, P=0.020). Compared with the low exposure category, significant SRRs for total cancer sites was obtained for high PCP exposure category (SRR=1.61, 95%CI=1.59-1.62). Most SRR values of the cancer sites were significantly increased as exposure grade elevated and exposure time extended. Conclusion: The present study found that community residents living in the PCP contaminated area had increased risk of cancers. Leukemias, lymphomas and nasopharyngeal and esophageal cancers are most possibly associated with PCP exposure.

A magnetostrictive self-powered biosensor based on Au-BaTiO3-FeGa & PDMS

Qiang Zhang,Meng Xu,Yan Liu,Chunyan Zhang,Rui Zhang,Zhiyuan Fu,Jianlong Ji,Riguang Zhang,Shengbo Sang 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.117 No.-

Piezoelectric flexible sensors have been used to detect biomolecules such as sweat and glucose because oftheir passive, simple structure and high sensitivity. This paper proposes a novel flexible piezoelectric Au-BaTiO3-FeGa & PDMS biosensor in which magnetostrictive deformation amplifies the surface stress generatedby biomolecules combining on the thin film. The modification process of bovine serum albumin(BSA) binding with the sensor was initially determined by the first principles approach. Then, the sensingmechanism was verified by finite-element simulation. Based on the simulation results, flexible Au-BaTiO3-FeGa & PDMS biosensors were prepared, modified, and measured. The structure, modification,and detection of the sensors were analyzed by digital microscopy, Fourier transform infrared spectrometry(FTIR), atomic force microscopy (AFM), and scanning electron microscopy (SEM). The responses ofthe biosensors detecting different BSA solution concentrations under magnetic fields were then investigated. Experimental results indicate that the biosensor has the highest sensitivity under a magnetic fieldof 30 mT.

A novel multi-physics field optimization method for GaN HEMT circuit design

Zhang, Rui,Wang, Yibo,Xu, Honghua The Korean Institute of Power Electronics 2021 JOURNAL OF POWER ELECTRONICS Vol.21 No.3

A device modeling and topology design method suitable for high-power-density and high-temperature applicationare important to develop with the development of devices. A novel multi-physics analysis model based on GaN high electron mobility transistor (HEMT) is proposed in this paper. The coupled electromagnetic and electrothermal model for GaN HEMT is beneficial to simulate a device's external characteristic waveform affected by different stray parameters in the surrounding. In addition, the proposed method can accurately reflect the switching loss and transient switching process of GaN HEMT.

Genetic diversity and population structure in five Inner Mongolia cashmere goat populations using whole-genome genotyping

Zhang Tao,Wang Zhiying,Li Yaming,Zhou Bohan,Liu Yifan,Li Jinquan,Wang Ruijun,Lv Qi,Li Chun,Zhang Yanjun,Su Rui 아세아·태평양축산학회 2024 Animal Bioscience Vol.37 No.7

Objective: As a charismatic species, cashmere goats have rich genetic resources. In the Inner Mongolia Autonomous Region, there are three cashmere goat varieties named and approved by the state. These goats are renowned for their high cashmere production and superior cashmere quality. Therefore, it is vitally important to protect their genetic resources as they will serve as breeding material for developing new varieties in the future.Methods: Three breeds including Inner Mongolia cashmere goats (IMCG), Hanshan White cashmere goats (HS), and Ujimqin white cashmere goats (WZMQ) were studied. IMCG were of three types: Aerbas (AEBS), Erlangshan (ELS), and Alashan (ALS). Nine DNA samples were collected for each population, and they were genomically re-sequenced to obtain high-depth data. The genetic diversity parameters of each population were estimated to determine selection intensity. Principal component analysis, phylogenetic tree construction and genetic differentiation parameter estimation were performed to determine genetic relationships among populations.Results: Samples from the 45 individuals from the five goat populations were sequenced, and 30,601,671 raw single nucleotide polymorphisms (SNPs) obtained. Then, variant calling was conducted using the reference genome, and 17,214,526 SNPs were retained after quality control. Individual sequencing depth of individuals ranged from 21.13× to 46.18×, with an average of 28.5×. In the AEBS, locus polymorphism (79.28) and expected heterozygosity (0.2554) proportions were the lowest, and the homologous consistency ratio (0.1021) and average inbreeding coefficient (0.1348) were the highest, indicating that this population had strong selection intensity. Conversely, ALS and WZMQ selection intensity was relatively low. Genetic distance between HS and the other four populations was relatively high, and genetic exchange existed among the other four populations.Conclusion: The Inner Mongolia cashmere goat (AEBS type) population has a relatively high selection intensity and a low genetic diversity. The IMCG (ALS type) and WZMQ populations had relatively low selection intensity and high genetic diversity. The genetic distance between HS and the other four populations was relatively high, with a moderate degree of differentiation. Overall, these genetic variations provide a solid foundation for resource identification of Inner Mongolia Autonomous Region cashmere goats in the future. Objective: As a charismatic species, cashmere goats have rich genetic resources. In the Inner Mongolia Autonomous Region, there are three cashmere goat varieties named and approved by the state. These goats are renowned for their high cashmere production and superior cashmere quality. Therefore, it is vitally important to protect their genetic resources as they will serve as breeding material for developing new varieties in the future. Methods: Three breeds including Inner Mongolia cashmere goats (IMCG), Hanshan White cashmere goats (HS), and Ujimqin white cashmere goats (WZMQ) were studied. IMCG were of three types: Aerbas (AEBS), Erlangshan (ELS), and Alashan (ALS). Nine DNA samples were collected for each population, and they were genomically re-sequenced to obtain high-depth data. The genetic diversity parameters of each population were estimated to determine selection intensity. Principal component analysis, phylogenetic tree construction and genetic differentiation parameter estimation were performed to determine genetic relationships among populations. Results: Samples from the 45 individuals from the five goat populations were sequenced, and 30,601,671 raw single nucleotide polymorphisms (SNPs) obtained. Then, variant calling was conducted using the reference genome, and 17,214,526 SNPs were retained after quality control. Individual sequencing depth of individuals ranged from 21.13× to 46.18×, with an average of 28.5×. In the AEBS, locus polymorphism (79.28) and expected heterozygosity (0.2554) proportions were the lowest, and the homologous consistency ratio (0.1021) and average inbreeding coefficient (0.1348) were the highest, indicating that this population had strong selection intensity. Conversely, ALS and WZMQ selection intensity was relatively low. Genetic distance between HS and the other four populations was relatively high, and genetic exchange existed among the other four populations. Conclusion: The Inner Mongolia cashmere goat (AEBS type) population has a relatively high selection intensity and a low genetic diversity. The IMCG (ALS type) and WZMQ populations had relatively low selection intensity and high genetic diversity. The genetic distance between HS and the other four populations was relatively high, with a moderate degree of differentiation. Overall, these genetic variations provide a solid foundation for resource identification of Inner Mongolia Autonomous Region cashmere goats in the future.

TSHR Variant Screening and Phenotype Analysis in 367 Chinese Patients With Congenital Hypothyroidism

Zhang Hai-Yang,Wu Feng-Yao,Li Xue-Song,Tu Ping-Hui,Zhang Cao-Xu,Yang Rui-Meng,Cui Ren-Jie,Wu Chen-Yang,Fang Ya,Yang Liu,Song Huai-Dong,Zhao Shuang-Xia 대한진단검사의학회 2024 Annals of Laboratory Medicine Vol.44 No.4

Background: Genetic defects in the human thyroid-stimulating hormone (TSH) receptor (TSHR) gene can cause congenital hypothyroidism (CH). However, the biological functions and comprehensive genotype–phenotype relationships for most TSHR variants associated with CH remain unexplored. We aimed to identify TSHR variants in Chinese patients with CH, analyze the functions of the variants, and explore the relationships between TSHR genotypes and clinical phenotypes. Methods: In total, 367 patients with CH were recruited for TSHR variant screening using whole-exome sequencing. The effects of the variants were evaluated by in-silico programs such as SIFT and polyphen2. Furthermore, these variants were transfected into 293T cells to detect their Gs/cyclic AMP and Gq/11 signaling activity. Results: Among the 367 patients with CH, 17 TSHR variants, including three novel variants, were identified in 45 patients, and 18 patients carried biallelic TSHR variants. In vitro experiments showed that 10 variants were associated with Gs/cyclic AMP and Gq/11 signaling pathway impairment to varying degrees. Patients with TSHR biallelic variants had lower serum TSH levels and higher free triiodothyronine and thyroxine levels at diagnosis than those with DUOX2 biallelic variants. Conclusions: We found a high frequency of TSHR variants in Chinese patients with CH (12.3%), and 4.9% of cases were caused by TSHR biallelic variants. Ten variants were identified as loss-of-function variants. The data suggest that the clinical phenotype of CH patients caused by TSHR biallelic variants is relatively mild. Our study expands the TSHR variant spectrum and provides further evidence for the elucidation of the genetic etiology of CH.

Targeting cathepsin L in the regulation of apoptosis in peripheral T-cell lymphoma

Zhang Rui,Ruan Yanjie,Zhao Yiming,Jin Fengbo,Yang Mingzhen,Zhai Zhimin 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.3

Backgrounds Peripheral T-cell lymphoma (PTCL) is a heterogeneous lymphoproliferative neoplasms derived from post-thymic T lymphocytes and mature NK cells. Although the incidence is rare, PTCL is frequently recurrence with pore prognosis. Cathepsin L is a member of cysteine proteases strongly related to the carcinogenesis and progression of malignant tumors. Objectives To test the expression of cathepsin L in PTCL and to clarify the possible role of cathepsin L in the pathogenesis of PTCL. Results The public datasheets showed a significant increase of cathepsin L in PTCL than normal T-cells. This result was confirmed by the collected tumor samples, in which the pathological changes and the immunohistochemical test were consistent with PTCL diagnosis. Two PTCL cell lines, Jurkat and Hut78, with a stable knockdown of cathepsin L were successfully established. The RNAseq analysis reported a total of eight co-differentially expressed genes between cathepsin Llow Jurkat vs vector Jurkat and cathepsin Llow Hut78 vs vector Hut78 two datasheets. The apoptosis array reported abnormal expression of several pro-apoptosis proteins including Fas, Bax, sTNF-R1, IGFBP-6, etc. According to the gene function enrichment analysis, p53 signaling pathway was involved in loss of cathepsin L in PTCL cells in vitro. In addition, the levels of above differentially expressed genes/proteins were finally checked, and our data confirmed an alteration of p53, apoptosis regulation-related factors (PAK4, SESN2) and apoptosis-related factors (Fas, Bax, sTNF-R1, and IGFBP-6). Conclusion Taken together, we demonstrated a role of cathepsin L in apoptosis by regulation of p53 signaling pathway. Our finding might provide a new possible candidate drug target for clinical treatment of PTCL in future. Backgrounds Peripheral T-cell lymphoma (PTCL) is a heterogeneous lymphoproliferative neoplasms derived from post-thymic T lymphocytes and mature NK cells. Although the incidence is rare, PTCL is frequently recurrence with pore prognosis. Cathepsin L is a member of cysteine proteases strongly related to the carcinogenesis and progression of malignant tumors. Objectives To test the expression of cathepsin L in PTCL and to clarify the possible role of cathepsin L in the pathogenesis of PTCL. Results The public datasheets showed a significant increase of cathepsin L in PTCL than normal T-cells. This result was confirmed by the collected tumor samples, in which the pathological changes and the immunohistochemical test were consistent with PTCL diagnosis. Two PTCL cell lines, Jurkat and Hut78, with a stable knockdown of cathepsin L were successfully established. The RNAseq analysis reported a total of eight co-differentially expressed genes between cathepsin Llow Jurkat vs vector Jurkat and cathepsin Llow Hut78 vs vector Hut78 two datasheets. The apoptosis array reported abnormal expression of several pro-apoptosis proteins including Fas, Bax, sTNF-R1, IGFBP-6, etc. According to the gene function enrichment analysis, p53 signaling pathway was involved in loss of cathepsin L in PTCL cells in vitro. In addition, the levels of above differentially expressed genes/proteins were finally checked, and our data confirmed an alteration of p53, apoptosis regulation-related factors (PAK4, SESN2) and apoptosis-related factors (Fas, Bax, sTNF-R1, and IGFBP-6). Conclusion Taken together, we demonstrated a role of cathepsin L in apoptosis by regulation of p53 signaling pathway. Our finding might provide a new possible candidate drug target for clinical treatment of PTCL in future.

Equivocal Association of RAD51 Polymorphisms with Risk of Esophageal Squamous Cell Carcinoma in a Chinese Population

Zhang, Shu-Xiang,Yang, Shan,Xu, Chang-Qing,Hou, Rui-Ping,Zhang, Chuan-Zhen,Xu, Cui-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Aim: To study the contribution of genetic variation in RAD51 to risk of esophageal squamous cell carcinoma (ESCC). Methods: Three single nucleotide polymorphisms (SNPs) in RAD51 (rs1801320, rs4144242 and rs4417527) were genotyped in 316 ESCC patients and 316 healthy controls in Anyang area of China using PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Demographic variables between cases and controls were statistically compared by T test and Chi-square test. Hardy-Weinberg equilibrium was evaluated by the Chi-square test. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated to measure any association with ESCC. Haplotype frequencies were estimated by Phase 2.1. Result: The genotype frequencies of rs1801320, rs4144242 and rs4417527 in patients with ESCC demonstrated no significant differences from those in control group (P>0.05). When the haplotypes of these three SNPs were constructed and their relationships with ESCC risk investigated, however, CGG was observed to increase the risk (P=0.020, OR=2.289). Conclusions: There was no association between the three SNPs of RAD51 and ESCC susceptibility in our Chinese population. However, the CGG haplotype might be a risk factor.

Poster Session : Cytoprotective activity of carpinus tschonoskii against H2O2 induced oxidative stress

( Rui Zhang ),( Kyoung Ah Kang ),( Mei Jing Piao ),( Ho Jin You ),( Byoung Sam Yoo ),( Young Taek Yang ),( Jin Won Hyun ) 생화학분자생물학회 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-