A Tissue-Engineered Conduit for Urinary Diversion Using Bone Marrow Mesenchymal Stem Cells and Bladder Acellular Matrix

Yunhe Xiong,Wen-Biao Liao,Sixing Yang,Lingchao Meng,Chao Song 한국조직공학과 재생의학회 2015 조직공학과 재생의학 Vol.12 No.3

Ileal conduits are commonly used in bladder cancer treatment and in pediatric patients who require urinary diversion surgeries. We constructed a tissue-engineered conduit using bone marrow mesenchymal stem cells (BMSCs) and a bladder acellular matrix (BAM) and transplanted it into rabbits for urinary diversion to evaluate the feasibility of its clinical application. Rabbit BMSCs were isolated, expanded in vitro, and were then treated with a conditional medium for 3 weeks, allowing the BMSCs to transform into urothelium-like cells. These cells were seeded onto BAM and cultured for another week. The cell-matrix grafts were then sewn into a conduit approximately 4 cm in length and 1 cm in diameter and were implanted as conduits for urinary diversion in 12 male rabbits. Rabbits were sacrificed at 1, 2, and 4 week after operation. Histologic examinations were performed using hematoxylin and eosin staining and tissue structures were evaluated by using immunohistochemistry. BMSCs can transform into urothelium-like cells that express the urothelium-specific proteins uroplakin 1A (UPK1A), cytokeratin 7 (CK7), and cytokeratin 13 (CK13). These cells are able to generate epithelial coverage in the conduit lumen based on BAM. Immunohistochemistry showed positive staining with AE1/AE3, UPK1A, and tight junction protein 1 (ZO-1), indicating the presence of mature and functional epithelial cells on the lumen of the conduit. BMSCs may represent a new source cell for urinary tissue engineering, and it is feasible to construct a conduit with transformed BMSCs and BAM for urinary diversion in rabbits.

Comparative study of the popularity of urban parks in Harbin and Vladivostok

Yunhe Ding,Liang Mao,Volovik Anna Korea Institute for Humanities and Social Sciences 2024 Journal of design futures (JDF) Vol.1 No.-

Background: The traditional assessment of urban green spaces is measured only by the Park's compliance with specific standards, including physical measurements such as the number of green spaces and the area per capita. However, this approach does not consider the sociopersonal parameters responsible for the indicators of a person's emotional attitude toward the Park, which can increase the attendance of city parks. Objectives: This study summarizes the theoretical knowledge gained in the field of park attendance, selects the popularity of urban parks as a subject not previously studied, and uses the popularity model of urban parks to analyze the popularity of urban parks in Harbin (China) and Vladivostok (Russia). Methods: By comparing the survey data and analyzing the data obtained by constructing a model of the popularity of urban parks, the results of the model were obtained, and the error of the resulting model was estimated for various sets of factors affecting the popularity and accessibility of urban parks. Results: The results show that both physical and socio-personal factors influence the popularity and accessibility of the Park, and it can also be said that if the Park does not have proper popularity among the population, then perceived accessibility is an essential factor in visiting it. These results have implications for the design of new urban parks. Conclusions: This study establishes a crosscultural framework aiding architects in addressing park popularity complexities, and paves the way for future research to refine critical factors and develop advanced models considering diverse city-specific elements.

Design Sensitivity Analysis of Static Aeroelastic Stresses via Iterative Adjoint-Based Method

Yunhe Cheng,Qin Sun 한국항공우주학회 2021 International Journal of Aeronautical and Space Sc Vol.22 No.6

Design sensitivity analysis of static aeroelastic stresses constitutes an important issue for the gradient-based integrated design optimization of wing structure, and can be analytically performed using a new modified stiffness matrix. However, introducing a modified stiffness matrix into the calculation will give rise to additional computational effort. Additionally, the direct method is incompetent for the problem containing a large number of design variables. In this research, a novel iterative adjoint-based method is presented for stress sensitivity analysis of flexible wing, the basis of which lies in the coupling between aerodynamic potential-flow panel model and structural finite element (FE) model. By virtue of an iterative approach for evaluating adjoint variable, we can obviate the modification to original stiffness matrix. Moreover, the adjoint-based method is more superior to the direct method when there are a large number of design variables. To demonstrate the verification of the algorithms, a rectangular wing, a swept wing and a large-scale unmanned aerial vehicle (UAV) are employed as numerical examples, and the design sensitivities of element equivalent stresses are calculated and analyzed.

A Study on the Potential of UGC New Media Platforms for Art Education: A Case Comparison Based on Wikipedia and Bilibili

장윤학(Yunhe Zhang) 학습자중심교과교육학회 2023 학습자중심교과교육연구 Vol.23 No.15

목적 본 연구의 목적은 UGC 모델에 따른 뉴미디어 플랫폼인 위키백과와 삐리삐리의 비교를 통해 사용자가 콘텐츠 편집과 평론에 참여하는 가운데 예술교육이 어떻게 실현될 수 있는지 탐구하는 것이다. 방법 이를 위해 UGC 모델의 뉴미디어 플랫폼인 위키백과와 삐리삐리게 사례 비교법을 적용했다. 미디어 편집 방식, 지식 링크, 미디어 참여자 등 몇 가지 측면을 구체적으로 분석해 두 플랫폼을 비교 연구한다. 데이터를 수집하고 위키백과 및 삐리삐리 플랫폼에 대한 자세한 정보를 얻기 위해 본 연구에서는 둘의 콘텐츠 특성과 사용자 상호 작용 모드 등 관련 정보를 수집하여 둘 사이의 차이점을 발견했다. 결과 연구결과는 다음과 같다. 첫째, 사용자가 어떤 예술가나 예술작품에 대한 자신의 관점이나 예술지식을 텍스트 편집을 통해 공유하는 것은 예술에 관한 내용이나 정보에 대해 고민한 후의 행위이며, 그 자체로 예술교육의 의미가 있다. 둘째, 플랫폼 운영자에게는 플랫폼의 영향력을 확대하고 더 많은 그룹을 플랫폼의 활동에 참여시킬 수 있으며, 이는 예술을 대중적으로 전파하는 행위이기도 한다. 셋째, 시각정보가 폭발하는 오늘날 UGC 플랫폼에서 문자편집과 시각정보가 어떻게 더 잘 통합될 수 있는지 더 많은 고민이 필요하고 대중의 예술교육에 더 많은 기여를 할 수 있다. 결론 분석 결과를 바탕으로 UGC 모델에 따른 뉴미디어 플랫폼인 위키백과와 삐리삐리가 예술교육에 미치는 의미와 발전 가능성을 설명했다. 비교 분석을 통해 둘 사이의 유사점과 차이점을 발견하여 둘의 특성과 장단점을 깊이 이해하고 뉴미디어 플랫폼을 통한 예술 교육의 발전과 응용에 유익한 시사점을 제공한다. 비교 분석을 통해 이들의 공통점과 차이점을 파악하여 이들의 특징, 장점과 단점을 전면적으로 파악할 수 있다. 이는 뉴미디어 플랫폼을 통해 예술 교육을 개발하고 활용할 수 있는 귀중한 견해를 제공할 수 있다. Objectives The purpose of this study is to explore how art education can be achieved in user participation in editing content and comments by comparing new media platforms under the UGC model-Wikipedia and Bilibili. Methods For this, a case comparison method was applied to Wikipedia and Bilibili, two new media sites that follow the UGC model. The two platforms were compared by carefully examining a number of factors, including media editing techniques, knowledge linkages, and media participants. To gather data and gain comprehensive insights into the Wikipedia and Bilibili platforms, this research identified and compared the similarities and differences between the two based on relevant information, including content characteristics and user interaction patterns. Results The results of the study are as follows. First, for users, sharing their opinions about an artist or an art work or gaining art knowledge by editing text is an act of thinking about art-related content or information afterwards, which itself has the meaning of art education. Second, for the platform operator, it can expand the influence of the platform and attract more groups to participate in the activities of the platform, which is also an act of mass communication of art. Third, in today's visual information explosion, it is necessary to further consider how text editing and visual information can be better combined in the UGC platform to make more contributions to art education for the public. Conclusions Based on the results of the analysis, the significance of the new media platforms in the UGC model- Wikipedia and Bilibili-for art education and the potential for development are illustrated. By conducting a comparative analysis, the similarities and differences between the two can be identified, allowing for a comprehensive understanding of their characteristics, benefits, and drawbacks. This can provide valuable insights for the development and utilization of art education through new media platforms.

XIAO is involved in the control of organ size by contributing to the regulation of signaling and homeostasis of brassinosteroids and cell cycling in rice

Jiang, Yunhe,Bao, Liang,Jeong, So‐,Yoon,Kim, Seong‐,Ki,Xu, Caiguo,Li, Xianghua,Zhang, Qifa Blackwell Publishing Ltd 2012 The Plant journal Vol.70 No.3

<P><B>Summary</B></P><P>Organ size is determined by cell number and size, and involves two fundamental processes: cell proliferation and cell expansion. Although several plant hormones are known to play critical roles in shaping organ size by regulating the cell cycle, it is not known whether brassinosteroids (BRs) are also involved in regulating cell division. Here we identified a rice T‐DNA insertion mutant for organ size, referred to as <I>xiao</I>, that displays dwarfism and erect leaves, typical BR‐related phenotypes, together with reduced seed setting. <I>XIAO</I> is predicted to encode an LRR kinase. The small stature of the <I>xiao</I> mutant resulted from reduced organ sizes due to decreased cell numbers resulting from reduced cell division rate, as supported by the observed co‐expression of <I>XIAO</I> with a number of genes involved in cell cycling. The <I>xiao</I> mutant displayed a tissue‐specific enhanced BR response and greatly reduced BR contents at the whole‐plant level. These results indicated that XIAO is a regulator of BR signaling and cell division. Thus, XIAO may provide a possible connection between BRs and cell‐cycle regulation in controlling organ growth.</P>

Torsional Vibration Analysis of Hydro-Generator Set Considered Electromagnetic and Hydraulic Vibration Resources Coupling

Zhiqiang Song,Yunhe Liu,Pengcheng Guo,Jianjun Feng 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.7

The torsional vibration characteristics of the hydro-generator set considering the electromagnetic and hydraulic vibration resources coupling are studied. The moment of rotation consists of water flow driving and magnetic torques caused by the air gap magnetic field in the generator rotor in the case of ignoring the mechanical friction. A coupling torque vibration model of hydro-generator shaft system was established. The model considered the coupling action of hydraulic and electromagnetic excitations, as well as the elastic effect of rotor spiders. The influences of the rotor moment inertia, rotor stiffness, and mass of turbine water added into the torsional natural vibration of the unit shaft system were studied. The effect of hydraulic excitation frequency on torsional vibration response was also investigated. The frequency response curves of shaft torque, torsional vibration angle, and electromagnetic torque were presented. The electromagnetic and hydraulic coupling resonance was studied when hydraulic excitation frequency was equal to zero and first-order frequencies. The excitation electric current and internal active power angle on torsional vibration was further analyzed. This study provides an analysis model and method considering the coupling of electromagnetic and hydraulic vibration resources for design and stable operation of water turbine generator sets.

Preparation and Characterization of a Novel Hyperbranched Poly(aryl ether ketone) Terminated with Cobalt Phthalocyanine to be Used for Oxidative Decomposition of 2,4,6-Trichlorophenol

Lei Lei,Yunhe Zhang,Xiujie Li,Jianxin Mu,Guibin Wang,Zhenhua Jiang,Shuling Zhang 한국고분자학회 2010 Macromolecular Research Vol.18 No.4

A novel hyperbranched poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-HPAEK)was prepared using 1,3,5-tris[4-(4-fluorobenzoyl) phenoxy] benzene (B3), as a ‘core’ molecule, 4,4'-(hexafluoroisopropylidene)diphenol (6F-BPA) as an A2 monomer, and cobalt phthalocyanine as an end-capping reagent. CoPc-THPAEK showed good solubility, thermal stability and catalytic activity in the oxidative decomposition of 2,4,6-trichlorophenol (TCP) under H2O2 or KHSO5. Moreover, the oxidative effect of KHSO5 was superior to that of H2O2,and the catalytic activity of CoPc-T-HPAEK was much higher than that of linear poly(aryl ether ketone) terminated with cobalt phthalocyanine (CoPc-T-LPAEK) and linear poly(aryl ether ketone) with a cobalt phthalocyanine pendant unit (CoPc-S-LPAEK).

Experimental and Analytical Study of Eccentrically Braced Frames Combined with High-Strength Steel

Shen Li,Yunhe Liu,JianBo Tian 한국강구조학회 2018 International Journal of Steel Structures Vol.18 No.2

In the structure of high-strength steel composite eccentrically braced steel frames (HSS–EBFs), the links and braces are made of Q345 steel, while the non-energy-dissipation segments (columns and beams) are made of high-strength steel (HSS). HSS reduces the cross-section of the members and increases the economic effi ciency. Here, four groups of K-HSS–EBFs are designed by performance-based plastic design method in this paper, which includes 5-storey, 10-storey, 15-storey and 20-storey, and each group contain four diff erent link length (900, 1000, 1100 and 1200 mm). The cyclic test loading was applied to 1:2 scale three-storey K-type HSS–EBFs (K-HSS–EBFs) with shear links to investigate their seismic performance. The results indicate that the as-prepared K-HSS–EBF structure exhibits excellent bearing capacity, ductility, and energy dissipation. We also fi nd that the fracture of the link web in the second storey led to the degradation of the load-carrying capacity. The non-designated yield members remained in the elastic stage, whereas the links ultimately experience inelastic rotations, and thus dissipate the energy in the K-HSS–EBFs. Moreover, nonlinear pushover analyses and nonlinear dynamic analyses are conducted, and the loading capacity, link rotations, ductility, interstory drifts and failure mode under rare earthquake of all models are compared. The results indicate that K-HSS–EBFs with diff erent link length have similar deformation characteristic and failure mode under pushover analysis or rare earthquakes, and the interstory drifts, link rotations and ductility of HSS–EBFs are increased with rising the link length.

Effects of α-Galactosidase Supplementation on Performance and Energy Metabolism for Broilers Fed Corn-non-dehulled Soybean Meal Diets

Zhang, Bo,Cao, Yunhe,Chen, Yiqun,Li, Yihang,Qiao, Shiyan,Ma, Yongxi Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.10

To study the effects of ${\alpha}$-galactosidase (${\alpha}$-Gal) supplementation on performance and energy metabolism, 216 Arbor Acres male broilers were placed in 36 cages of 6 birds each and allotted to 4 diets for 42 d, with 0-21 d as starter period and 22-42 d as grower period. The 4 diets were based on corn non-dehulled soybean meal in a $2{\times}2$ factorial arrangement, with 2 levels of ${\alpha}$-Gal (0 vs. 60 U/kg feed) and 2 levels of ME (normal metabolizable energy (NME) and low metabolizable energy (LME)). Bird performance was obtained at 21 and 42 d of age with samples of feces collected for nutrient digestibility from 19-21 d and 40-42 d. At 21 and 42 d, 1 bird from 6 cages of each treatment was killed to determine liver weight, intestinal pH and chyme viscosity. With the addition of ${\alpha}$-Gal the 42 d body weight (BW) and 0-42 d average daily gain (ADG) were significantly improved (p<0.05). Average daily feed intake (ADFI) of birds fed the LME diet was significantly increased compared to those fed the NME diet during starter (p<0.01) and grower (p<0.05) periods and overall (p<0.01). There was an interaction of ${\alpha}-Gal{\times}ME$ on 0-21 d ADFI (p<0.01). Supplementation of ${\alpha}$-Gal significantly improved (p<0.01) feed efficiency during the grower period and overall. Feed efficiency of birds fed the LME diet was significantly decreased (p<0.05) compared to those fed the NME diet during the starter period and overall. With the addition of ${\alpha}$-Gal apparent metabolizable energy (AME) was improved (p<0.01) by 2.1% and 1.8% during starter and grower periods, respectively. There was a main effect (p<0.05) of ${\alpha}$-Gal on the digestion of neutral detergent fiber (NDF) during the starter period and crude protein (CP), NDF and acid detergent fiber (ADF) during the grower period. With the addition of ${\alpha}$-Gal, the relative weight of liver was reduced (p<0.01) during the two phases. The duodenal and jejunal pH were significantly decreased (p<0.01) with the supplementation of ${\alpha}$at the two phases. ${\alpha}$-Gal addition reduced (p<0.01) chyme viscosity of the ileum during the starter and grower periods. Overall, ${\alpha}$-Gal showed a major effect on nutrient efficiency, improved ADG and feed efficiency, whereas LME decreased feed efficiency. The incorporation of ${\alpha}$-Gal into a LME diet could at least partially offset ME deficiency of non-dehulled soybean meal.

Study on Mechanical Properties of Press-Bending Members Based on Transmission Towers Diagonal Member Reinforcement In-Situ

Zhenke Xin,Huanhuan Wei,Yunhe Liu,Gang Liang 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.4

According to the reinforcement requirements of load-bearing members of existing transmission tower, a new type of in-situ reinforcement scheme for the angle steel T-shaped composite section is proposed. Through the compression test of 10 groups of members, the infl uence of slenderness ratio, the number of fi xtures, the number of bolt rows and other parameters on the failure mode and bearing performance of members was studied. A simulation model was established using ABAQUS software, the load-displacement curve, failure models and other aspects verify the reliability and validity of simulation results. The results show that when the slenderness ratio of member reaches a certain value, the ultimate bearing capacity of member reinforced with the reinforcement angle steel of same specifi cation and strength can be multiplied and increased several times, the slenderness ratio has little eff ect on the force transmission effi ciency of reinforcement angle steel. The number of bolt rows has a small eff ect on the ultimate bearing capacity of member, the increase rate of ultimate bearing capacity, the failure mode and the force transmission effi ciency of reinforcement angle steel, the infl uence of strength of reinforcement angle steel, the specifi cation of reinforcement angle steel on the ultimate bearing capacity, and the increase rate of ultimate bearing capacity of member obviously, the higher strength and specifi cation of reinforcement, the greater the ultimate bearing capacity of reinforced member, and the higher the rate of improvement of ultimate bearing capacity, the smaller the thickness of fi ller plate, that is the smaller the eccentricity, the higher force transmission effi ciency of reinforcement. The research results can provide experimental and theoretical basis for the reinforcement of tower structure and engineering design.