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하윤석,강성진,허소영,이지현,김민정,김종국,김광수 고신대학교 의과대학 2009 고신대학교 의과대학 학술지 Vol.24 No.1
Thunderclap headache is a hyperacute, severe headache that is so named because the pain strikes suddenly and severely, and is sometimes a sign of a medical emergency. We report a case of pheochromocytoma in the urinary bladder in a 48-year-old woman who presented with recurrent thunderclap headache after voiding and exercise. So we think thunderclap headache during or immediately after micturition may be an important diagnostic clue of bladder pheochromocytoma.
한국 자귀나무 집단의 6-phosphogluconate dehydrogenase에 의한 다형현상
허만규,윤숙경,허홍욱 釜山大學校 師範大學 1994 교사교육연구 Vol.29 No.-
The objectives of the present report were to determine the levels of genetic diversity among the characteristics of Albizzia juribrissin and to examine the organization of genetic variation within the species and the patterns of genetic differentiation between populations. Starch gel electrophoresis was used to examine the allozyme variation of 6-PGDH. The frequency of slow allele for the 6-PGDH locus was 5.9% on the average (range ; 0.0-18.5). The highest (0.2210) and lowest (0.0234) distance values were exhibited by the population pairs Goseong-Nonsan and Changhung-Daejeon, respectively. The average heterozygosity varied between 0.000 in Kyongju population and 0.291 in Chilkok population.
Effective Extraction of Oligomeric Proanthocyanidin (OPC) from Wild Grape Seeds
Huh Yun Suk,Hong Tae Hee,Hong Won Hi The Korean Society for Biotechnology and Bioengine 2004 Biotechnology and Bioprocess Engineering Vol.9 No.6
The Oligomeric proanthocyanidin (OPC) in green and black tea, grape seeds, grapes and wine has raised much attention but that OPC in wild grape seed remains to be intensively investigated. This study investigated the total OPC contents and total antioxidant activity of wild grape seeds and developed an efficient extraction process with various temperatures, solvent compositions and times. Also, a chromatography column packed with the Dia-ion HP-20 resin was used for further purification of the ope. The total OPC contents were determined with the Folin-Ciocalteu reagent, and the antioxidant activity using total antioxidant potential (TAP) and 1,1-dipheny|-2picrylhydrazy| (DPPH). The yield of final purified OPC was 1.78 (+)-catechin equivalent (CE) g/100 g, with $IC_{50}$ activities of TAP and DPPH of 31.60 and $15.70\;{\mu}\;g/mL$. These activities of the final purified OPC were about two times higher than that of the BHA used as a reference sample.
고효율 바이오물질 분리 및 농축을 위한 나노필터소자제작
허윤석 ( Yun Suk Huh ),최봉길 ( Bong Gil Choi ),홍원희 ( Won Hi Hong ) 한국화학공학회 2012 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.50 No.4
Here, we develop a new nanofilter device for the rapid and efficient separation of nanoparticles and biomolecules, exploiting the use of AAO mebrane with ordered nanopores in the range from 20 nm to 200 nm. Briefly, the chip comprises of a series of the upper and lower PDMS channels containing embedded inlet and outlet ports, and 50μm width microfluidic channel, and AAO membrane to be made the filtering zone. After assembling these components, the acrylate plastic plates were used to fix the device on the top and bottom side. When introducing the samples into the inlet ports of the upper PDMS channel, we were able to separate and concentrate the nanoparticles and target molecules at the filtering zone, and to elute the solutions containing the unwanted materials toward the lower PDMS channels normal to the direction of AAO membrane. To demonstrate the usefulness of the device we apply it to the SERS detection of nucleic acid sequences associated with Dengue virus serotype 2. We report a limit of detection for Dengue sequences of 300nM and show excellent enhancement of Raman signals from the filter zone of the nanofilter device.
Vesosomal drug delivery for controlled and sustained release of multiple components
Jingyeong Yun(윤진경),Deborah Lee(이드보라),Seoyoon Song(송서윤),Suheon Kim(김수헌),Mina Lee(이민아),Eunsu Kim(김은수),Sunhee Yoon(윤선희),Han-Ul Kim(김한울),Sejin Son(손세진),Hyun Suk Jung(정현석),Yun Suk Huh(허윤석),Tae-Joon Jeon(전태 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.11