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Synthesis and biological evaluation of the mimics of cis ligand for CD22
Yuki Sugamuna,Naoko Matsubara,Yuki Iwayama,Akiharu Ueki,Akihiro Imamura,Hiromune Ando,Takeshi Tsubata,Hideharu Ishida,Makoto Kiso 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
CD22 (siglec-2) is an accessory molecule of the B-cell receptor complex (BCR) that exertsnegative effects on receptor signaling. It is also well-documented that CD22 is a regulatoryprotein that sets a threshold for immune responses. The carbohydrate ligand recognized byCD22 is the sequence Neuα(2,6)Galβ(1,4)GlcNAc found on both neighboringglycoconjugate of the same cell (cis ligand) and on other cells that interact with B cells (transligands). Recently, we have reported that the C-9 amido derivative of sialic acid (GSC718;9-(4’-hydroxy-4-biphenyl)acetamido-9-deoxy-Neu5Gc-OBn) show a potent affinity andselectivity for CD22 than other siglecs such as MAG [1]. Moreover, the compoundpromoted the proliferation of B cells in vitro. As next step of our investigation, we intend toreinforce the promoting activity of GSC718 for B cell growth by chemical modification.Herein, we report the efficient synthesis of GSC718 analogs which have varied aglyconmoieties. To achieve the comprehensive synthesis of GSC718 analogs having varied aglycons, wereexamined every synthetic process to obtain a fine target compound. In case of thesialoside synthesis, the most time-consuming and troublesome process is thechromatographic separation of α-sialoside from other byproducts such as β−isomer, 2,3-enederivative etc. after glycosylation reaction with aglycon part. To improve this process, weemployed 1,5-lactam formation as the key step for separation because the lactam formation isknown to proceed only in α-sialoside [2]. At the beginning of the synthesis of targetmolecules, we synthesized a suitably modified sialic acid donor in good yields. Then, thesialyl donor was reacted with various 2-substituted-ethanols to give the mixtures of α- and β-glycosides and 2,3-ene derivative, which were subsequently advanced to 1,5-lactamformation. As we anticipated, 1,5-lactamized α-sialosides became isolable from themixtures due to its different polarity from the other byproducts. Finally, the obtained 1,5-lactamized silaosides were successfully converted into target structures via reaction sequenceincluding C9-midification with biphenyl amide group, lactam opening and globaldeprotection. The synthesized analogs were advanced to biological assay using B cells. In this poster presentation, we will also discuss the structure-activity relationships of thesynthesized analogs. [1] H. H. M. Abdu-Allah et al, Bioorg. Med. Chem. Lett. 2011, 19, 1966-1971. [2] H. Tanaka et al, Tetrahedron Lett. 2009, 50, 4478-4481.
( Yuki Nakao ),( Shinsuke Mikami ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Aeromonas species are well known to cause diarrhea. They also cause skin and soft tissue infections (SSTI) and sepsis in patients with hepatic diseases, diabetes mellitus, and immunocompromised status. The genus Aeromonas comprises gram-negative rods widely distributed in freshwater, estuarine and marine environments. Aeromonas infections are relatively frequent in warm tropical or subtropical climates. Although many reports concerning Aeromonas infections have been published from these areas close to the coast, a paucity of material is available on Aeromonas infections from hospitals in hilly and mountainous areas. Methods: We retrospectively investigated patients who were identifi ed from the clinical database of Miyoshi Central Hospital between December 2009 to June 2014. It is located at hilly and mountainous area in the north side of Hiroshima Prefecture. Results: Aeromonas species were isolated from 49 patients in a general hospital. Subjects included 29 males and 20 females, ranging in age from 0 to100 years (median age 69 years). They were isolated every month except for February. five patients were died. One of them died of skin and soft tissue infections caused by the Aeromonas species. Conclusions: Aeromonas infections may occur not only at coastal areas but also hilly and mountainous areas.
Yukie Tateno,Kahoru Kumagai,Ryunosuke Monden,Kotaro Nanba,Ayumi Yano,Eri Shiraishi,Alan R. Teo,Masaru Tateno 대한소아청소년정신의학회 2021 소아청소년정신의학 Vol.32 No.1
Objectives: Among the many intervention programs for children with autism spectrum disorder (ASD), the Early Start Denver Model (ESDM) is one of the few approaches that has succeeded in demonstrating clinical efficacy in randomized control trials. Here, we investigate the clinical efficacy of ESDM intervention in young children with ASD in a community setting within Japan. Methods: All subjects were children with ASD who received ESDM intervention during the study period. Each ESDM session lasted 75 min and occurred once per week for at least 12 weeks. The outcome measures consisted of the Kyoto Scale of Psychological Development (K-test), Aberrant Behavior Checklist-Japanese version (ABC-J), and the Clinical Global Impression-Severity scale (CGI-S). Results: Twenty-seven subjects (29.4±6.4 months old) received ESDM intervention that lasted for 8.0±2.6 months on average. The score on Language and Social developmental quotient on the K-test increased significantly after the intervention. The total scores on the ABC-J and CGI-S significantly decreased after completion of the ESDM intervention. Conclusion: Our results suggest that ESDM intervention could reduce the severity of distinct clinical features of ASD, such as impairments in social interaction and communication assessed by the K-test, and maladaptive behavior rated by the ABC-J and CGI-S. We believe that the ESDM adapted to each institution might become one of the standard options for children with ASD in Japan.
A tool for the design of DNA base sequences for molecular circuits
Yuki Yoshida,Takashi Nakakuki 제어로봇시스템학회 2017 제어로봇시스템학회 국제학술대회 논문집 Vol.2017 No.10
Designing a set of DNA base sequences for a large-scale molecular circuit is a difficult problem in the field of DNA computing. A DNA circuit frequently suffers from unintended DNA strand displacements among strands; this problem can ruin the desired function of a DNA circuit. In this study, our aim was to develop a computational tool for the design of DNA base sequences by means of an original algorithm based on mismatches in a DNA base sequence. Validity of the proposed algorithm is confirmed by practical redesign examples.
Yuki Tokutsu,Wakako Umene-Nakano,Takahiro Shinkai,Reiji Yoshimura,Tatsuya Okamoto,Asuka Katsuki,Hikaru Hori,Atsuko Ikenouchi-Sugita,Kenji Hayashi,Kiyokazu Atake,Jun Nakamura 대한정신약물학회 2013 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.11 No.1
Objective: Electroconvulsive therapy (ECT) has proven to be effective in treatment-resistant depression (TRD). In recent reports,70% to 90% of patients with TRD responded to ECT. However, post-ECT relapse is a significant problem. There are no studies investigating risk factors associated with reintroducing ECT in depressive patients after remission previously achieved with former ECT. The aim of the present study is to examine such risk factors using a sample of TRD patients. Methods: We conducted a chart review to examine patient outcomes and adverse events over short- and long-term periods. Forty-two patients met the criteria for major depressive disorder. Results: The response rate was 85.7% (36/42). There were no significant differences in the baseline characteristics of patients exhibiting remission, response or non-response. The rate of adverse events was 21.4% (9/42). Among 34 patients who were available for follow-up, 18 patients relapsed (relapse rate, 52.9%), and 6 patients were reintroduced to ECT. The patients’ age and age of onset were significantly higher in the re-ECT group than non re-ECT group. Conclusion: Our results suggest that older age and older age of onset might be considered for requirement of re-ECT after remission previously achieved with former ECT.
Teacher’s Class Design in the First Year Experience
Yuki Enkai 한양대학교 교육공학연구소 2009 학습과학연구 Vol.3 No.1
In recent years, the number of colleges that have class of the First Year Experience has increased in Japan. The First Year Experience is a kind of class which is held for university freshman. However, this class contents and way of practice ate still seeking, therefore we need to study and improve problems. In this paper, I studied about the points which teachers think important when they teach class of the First Year Experience. There were difference points that teacher think important, because they have each ideas for curriculum.
Yuki Tanaka,Hideo Dohra,Tomohiro Suzuki,Takeshi Kurokura,Masayuki Iigo,Fubito Toyama,Naoto Habu,Naotake Konno 한국유전학회 2017 Genes & Genomics Vol.39 No.12
The complete mitochondrial genome sequence of the wood-decaying fungus Fomitopsis palustris (Basidiomycete, Agaricomycotina) was determined by next-generation sequencing technology. The complete sequence of the circular mitochondrial DNA of F. palustris was 63,479 bp in length with a 75.98% AT content. The mitochondrial genome encoded 14 conserved proteins, 2 ribosomal RNAs, 26 transfer RNAs, and 19 additional open reading frames. The coxI and cob genes contained six and one group I introns, respectively, and encoded eight open reading frames, including seven intron-encoded endonucleases. The complete mitochondrial genome of F. palustris presented herein represents the first such report for brown rot basidiomycetes. In addition, the BLAST score ratio and phylogenetic analysis may open new avenues to understanding the evolutionary status of this fungus.