RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      • 좁혀본 항목

      • 좁혀본 항목 보기순서

        • 원문유무
        • 음성지원유무
        • 원문제공처
        • 등재정보
        • 학술지명
        • 주제분류
        • 발행연도
        • 작성언어
        • 저자

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • KCI등재SCOPUS

        A Rapid PCR-Based Approach for Molecular Identification of Filamentous Fungi

        Yuanyuan,Chen,Bernard,A.,Prior,Guiyang,Shi,Zhengxiang,Wang 한국미생물학회 2011 The journal of microbiology Vol.49 No.4

        In this study, a novel rapid and efficient DNA extraction method based on alkaline lysis, which can deal with a large number of filamentous fungal isolates in the same batch, was established. The filamentous fungal genomic DNA required only 20 min to prepare and can be directly used as a template for PCR amplification. The amplified internal transcribed spacer regions were easy to identify by analysis. The extracted DNA also can be used to amplify other protein-coding genes for fungal identification. This method can be used for rapid systematic identification of filamentous fungal isolates.

      • KCI등재SCOPUS

        Simplified Analytical Model for Investigating the Output Power of Solar Array on Stratospheric Airship

        Yuanyuan,Zhang,Jun,Li,Mingyun,Lv,Dongjie,Tan,Weiyu,Zhu,Kangwen,Sun 한국항공우주학회 2016 International Journal of Aeronautical and Space Sc Vol.17 No.3

        Solar energy is the ideal power choice for long-endurance stratospheric airships. The output performance of solar array on stratospheric airship is affected by several major factors: flying latitude, flight date, airship's attitude and the temperature of solar cell, but the research on the effect of these factors on output performance is rare. This paper establishes a new simplified analytical model with thermal effects to analyze the output performance of the solar array. This model consisting of the geometric model of stratospheric airship, solar radiation model and incident solar radiation model is developed using MATLAB computer program. Based on this model, the effects of the major factors on the output performance of the solar array are investigated expediently and easily. In the course of the research, the output power of solar array is calculated for five airship's latitudes of 0°, 15°, 30°, 45° and 60°, four special dates and different attitudes of five pitch angles and four yaw angles. The effect of these factors on output performance is discussed in detail. The results are helpful for solving the energy problem of the long endurance airship and planning the airline.

      • KCI등재SCOPUS

        An Enzymolysis-Assisted Agrobacterium tumefaciens-Mediated Transformation Method for the Yeast-Like Cells of Tremella fuciformis

        Yuanyuan,Wang,Danyun,Xu,Xueyan,Sun,Lisheng,Zheng,Liguo,Chen,Aimin,Ma 한국균학회 2019 Mycobiology Vol.47 No.1

        Agrobacterium tumefaciens-mediated transformation (ATMT), as a simple and versatile method, achieves successful transformation in the yeast-like cells (YLCs) of Tremella fuciformis with lower efficiency. Establishment of a more efficient transformation system of YLCs is important for functional genomics research and biotechnological application. In this study, an enzymolysis-assisted ATMT method was developed. The degradation degree of YLCs depends on the concentration and digestion time of Lywallzyme. Lower concentration ( 0.1%) of Lywallzyme was capable of formation of limited wounds on the surface of YLCs and has less influence on their growth. In addition, there is no significant difference of YLCs growth among groups treated with 0.1% Lywallzyme for different time. The binary vector pGEH under the control of T. fuciformis glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter was utilized to transform the enzymolytic wounded YLCs with different concentrations and digestion time. The results of PCR, Southern blot, quantitative real-time PCR (qRT-PCR) and fluorescence microscopy revealed that the T-DNA was integrated into the YLCs genome, suggesting an efficient enzymolysis-assisted ATMT method of YLCs was established. The highest transformation frequency reached 1200 transformants per 10 6 YLCs by 0.05% (w/v) Lywallzyme digestion for 15 min, and the transformants were genetically stable. Compared with the mechanical wounding methods, enzymolytic wounding is thought to be a tender, safer and more effective method.

      • KCI등재SCOPUS

        An Enzymolysis-Assisted Agrobacterium tumefaciens-Mediated Transformation Method for the Yeast-Like Cells of Tremella fuciformis

        ( Yuanyuan Wang ), ( Danyun Xu ), ( Xueyan Sun ), ( Lisheng Zheng ), ( Liguo Chen ), ( Aimin Ma ) 한국균학회 2019 Mycobiology Vol.47 No.1

        Agrobacterium tumefaciens-mediated transformation (ATMT), as a simple and versatile method, achieves successful transformation in the yeast-like cells (YLCs) of Tremella fuciformis with lower efficiency. Establishment of a more efficient transformation system of YLCs is important for functional genomics research and biotechnological application. In this study, an enzymolysis-assisted ATMT method was developed. The degradation degree of YLCs depends on the concentration and digestion time of Lywallzyme. Lower concentration (_0.1%) of Lywallzyme was capable of formation of limited wounds on the surface of YLCs and has less influence on their growth. In addition, there is no significant difference of YLCs growth among groups treated with 0.1% Lywallzyme for different time. The binary vector pGEH under the control of T. fuciformis glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter was utilized to transform the enzymolytic wounded YLCs with different concentrations and digestion time. The results of PCR, Southern blot, quantitative real-time PCR (qRT-PCR) and fluorescence microscopy revealed that the T-DNA was integrated into the YLCs genome, suggesting an efficient enzymolysis-assisted ATMT method of YLCs was established. The highest transformation frequency reached 1200 transformants per 10<sup>6</sup> YLCs by 0.05% (w/v) Lywallzyme digestion for 15 min, and the transformants were genetically stable. Compared with the mechanical wounding methods, enzymolytic wounding is thought to be a tender, safer and more effective method.

      • KCI등재SCOPUS

        Oil film stiffness and damping in an elastohydrodynamic lubrication line contact-vibration

        Yuanyuan,Zhang,Hua,iju,Liu,Caichao,Zhu,Mingyong,Liu,Chaosheng,Song 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.7

        An elastohydrodynamic lubrication line contact-vibration model is proposed to study the stiffness and damping of the oil film existed in the EHL contact region. An initial mutual approach between interacting surfaces, which deviates from the steady-state balanced position, is assumed under the free contact-vibration to predict the response of the mutual approach. An inertia term, which represents the acceleration of the gap motion, is added to the classical force balance equation to form the equation of motion of the mutual approach. Response of the mutual approach is solved based upon the solving of the contact-dynamic model. The oil stiffness is calculated according to the natural frequency of the response under damped and non-damped conditions, the latter of which represents dry contact conditions. The oil film damping is calculated in terms of the principle of the energy conservation which utilizes the whole history of response compared with the log decrement method. Effect of the normal load, the rolling speed and the amplitude of the regular sinusoidal surface waviness on the oil film stiffness, the contact stiffness and the oil film damping are studied. The study provides an insight on the oil film dynamic characteristics of lubricated contact-vibration problems which appear in gears, bearings, etc. The results show that the oil film damping factor decreases with the increasing normal load as well as the increasing rolling speed. The oil film stiffness increases with the normal load and decreases with the rolling speed. Also, compared to the contact stiffness, the oil film stiffness makes less contribution to the total stiffness. The surface waviness amplitude has little effect on the oil film stiffness and the oil film damping.

      • KCI등재SCOPUS

        Reliability assessment for multi-source data of mechanical parts of civil aircraft based on the model

        Yuanyuan,Guo,Youchao,Sun,Longbiao,Li,Xiaoming,Tang 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.7

        In view of the abnormal, missing and multi-source reliability data, a complete reliability data processing method for civil machinery parts are formed. The abnormal data in the original reliability data set is screened out by Pauta criterion. The vacant data is supplemented by linear interpolation. Each subjective index weight of multi-source data is determined by analytic hierarchy process (AHP) and Euclidean distance. Each objective index weight is determined by the discrete degree of the index weight. The comprehensive reliability model about a certain mechanical part with multi-source data is established and validated to be practical through case analysis. The proposed method obtains more accurate assessment of part reliability, and is thus valuable for the safe operation and maintenance of civil aircraft.

      • KCI등재SCOPUS

        A Rapid and Effective Colony PCR Procedure for Screening Transformants in Several Common Mushrooms

        Yuanyuan,Wang,Danyun,Xu,Dongmei,Liu,Xueyan,Sun,Yue,Chen,Lisheng,Zheng,Liguo,Chen,Aimin,Ma 한국균학회 2019 Mycobiology Vol.47 No.3

        In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant's tissue (1–10 lg) to 20 ll 0.25% Lywallzyme solution, and vortexing for 10 s followed by incubation at 34 C for 15 min. Finally, 2 ll of the suspension was used as templates to perform PCR and single target bands were successfully amplified from respective transformants of Tremella fuciformis, Pleurotus ostreatus, and Pleurotus tuber-regium. This procedure could be widely employed for screening transformants in mushroom transformation experiments.

      • KCI등재

        A Mannich base biosorbent derived from alkaline lignin for lead removal from aqueous solution

        Yuanyuan,Ge,Quanpeng,Song,Zhili,Li 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.23 No.-

        A Mannich base biosorbent (AML) was prepared by grafting alkaline lignin (AL) with methylamine and formaldehyde. The effects of dosages, reaction time, pH and temperature of reaction on the nitrogen content of AML were investigated. A maximum nitrogen content of the Mannich base could reach to 8.32%. It was characterized by scanning electron microscopy (SEM), element analysis, and gel permeation chromatography (GPC). Kinetic adsorption suggested AML possessed fast adsorption for Pb2+ and followed a second-order model. Adsorption equilibrium results indicated AML had a maximum uptake of 60.5 mg/g for Pb2+ which was 4.2-fold higher than AL. The AML has good potential for cleanup of wastewater.

      • KCI등재

        A selective and sensitive ‘‘naked-eye'' rhodamine-based ‘‘turn-on'' sensor for recognition of Hg2+ ion in aqueous solution

        Yuanyuan,Yan,Guodong,Ding,Hui,Xu 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.25 No.-

        A selective and sensitive ‘‘naked-eye'' rhodamine-based ‘‘turn-on'' chemosensor for detection of Hg2+ ion over other metal ions such as Ni2+, Ca2+, Ba2+, Cs+, Fe3+, Mn2+, Cd2+, Zn2+, Mg2+, K+, Ag+, Hg2+, Co2+, and Pb2+ in aqueous solution was prepared. Meanwhile, a new method for the synthesis of rhodamine amide directly from rhodamine hydrazide in the presence of NaH was reported for the first time. It was observed that once Hg2+ ion was added to the sensor 4, a 91.4-fold enhancement of the absorption at 570 nm was observed. Its selectivity toward Hg2+ ion was very high, and little interference was detected for other commonly coexistent metal ions. The fluorescent intensity was almost a constant minimal value when pH = 5–8 in aqueous solution. It demonstrated that Hg2+ could be detected at least down to 3 108 M. Moreover, the Hg2+-promoted selective desulfurization reaction was further confirmed by MS spectra. The present probe could be used to determine Hg2+ ion concentrations in aqueous environments.

      • KCI등재

        Sulfonated multi-walled carbon nanotubes for the removal of copper (II) from aqueous solutions

        Yuanyuan,Ge,Zhili,Li,Duo,Xiao,Piao,Xiong,Na,Ye 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.4

        Sulfonated multi-walled carbon nanotubes (s-MWCNTs) was prepared from purified multi-walled carbon nanotubes (p-MWCNTs) by concentrated H2SO4 at elevated temperature. The structure was characterized by SEM, FTIR, Raman, XPS, and BET. It could be dispersed steadily in water at a dosage of 1.0 mg/mL for a week. The adsorption performance of s-MWCNTs toward Cu(II) was investigated including the effects of pH and ionic strength. Results indicated the adsorption was much dependent on pH but not on ionic strength. The adsorption capacity for Cu(II) was enhanced 58.9% via the sulfonation. Moreover, the adsorption mechanisms were carefully analyzed by Freundlich and D-R models.

      맨 위로 스크롤 이동