Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122의 생산과 그 특성

최용석,옥승호,유주현,배동현 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.1

토양으로부터 분리한 Metarrhizium anisopliae (Metschn.) Sorok이 생산하는 biopolymer를 정제하여 분석하고, 그의 생산조건을 검토하였다. 탄소원으로 mannitol, 질소원으로는 polypepton을 사용했을 때 가장 많은 biopolymer를 생산하였고 K_2HPO_4 및 CaCl_2를 첨가함으로서 생산량이 더욱 증가하였다. 또한 이 biopolymer를 정제하여 그 분자량과 구성성분을 검토한 결과 Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122는 분자량이 1.7×10 exp (6)이고, C, H의 구성비가 1:2이며 미량의 N이 검출되었으나, 주 구성성분은 glucose와 galactose의 환원당으로 이루어져있는 전혀 새로운 biopolymer인 것으로 나타났다. To produce biopolymer, Metarrhizium anisopliae (Metschn.) Sorok was cultured in a medium containing glucose 1.0%, sucrose 2.0%, soluble starch 1.0%, yeast extract 0.5%, polypeptone 0.5%, K_2HPO_4 0.1%, MgSO_4·7H_2O 0.02%. The culture broth was centrifuged and the polymer was harvested by adding methanol to the culture supernatant. When three times of methanol was added, the polymer was coagulated and precipitated. Then it was further purifued through successive SK-1B, SA-20P, HP-20 column chromatographies. This polymer was designated as Biopolymer YU-122. C:H ratio of this Biopolymer YU-122 was 1:2 and small amount of N is detected by CHN analyzer. Glucose and galactose are main components of this polymer. Average molecular weight of this biopolymer was 1.7×10 exp (6) by Sepharose 4B gel permeation chromatography. Optimal condition for biopolymer production was investigated. When 5% of mannitol was used as a carbon source, and polypepton as a N source, highest productivity of biopolymer was achieved. C/N ratio as nutrient was also a major factor in polymer production and its optimal ratio was 3.

탈식민주의 관점에서 본 Heart of Darkness

김여훈 동의공업대학 2001 論文集 Vol.27 No.1

Joseph Conrad's Heart of Darkness has been studied by many critics since its first publication in 1899. Some have seen it as a great work of English literature while others have found many problems in it, including Chinua Achebe's racism and Edward Said's orientalism. Thus postcolonial theories are theoretical and critical strategies used to examine the culture, literature, politics, history of former colonies of the European empires, and their relation to the rest of the world. They also question such issues as racism, gender, exploitation, resistance, representation, difference, and responses to the influential master discourses of imperial Europe. Throughout the work, Marlow, as a persona of the author, describes the image of Africa as the uncivilized or savage side of the world. Although Marlow shows that the pagan rites and savage dances are not only done by the Africans but ironically by the Europeans as well, the savagery and uncivilization of the Africa is described as Marlow and Kurtz try to quickly escape. Imperialism is at the center of Heart of Darkness. By the 1890's most of the world's 'dark places' had been placed under European control. Conrad also criticized the European imperialism over the colonized countries and the cruelty of the Europeans-their utter darkness in his works. Heart of Darkness was one of the first novels to provide a critical view of European imperial activities. But as an author who lived in a time when some forms of racism seemed natural. Conrad was certainly not completely free from prejudice in his work, Heart of Darkness.

최근 8년간(2001-2008) 서울대학교치과병원 소아치과의 진료현황 및 수익분포 변화에 대한 조사

손유진,현홍근,김영재,김정욱,이상훈,김종철,한세현,장기택 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.1

The practice pattern of pediatric dentistry in the university dental hospital has been changing for the last several decades, due to socioeconomic changes, developments in dental materials and increasing number of private pediatric dental clinics. The purpose of this study is to survey the changing patterns of pediatric dental treatment in the university dental hospital and to present the future direction for pediatric dentistry. Patient distribution and practice trends were reviewed based on the OCS (order communication system) records of the department of pediatric dentistry, Seoul National University Dental Hospital from 2001 to 2008. Results were as follows; 1. The number of patients increased up to 2005 and decreased in 2006, and has remained constant up to 2008. 2. The proportion of preventive and orthodontic treatment increased and the proportion of restorative and pulpal treatment decreased. 3. In restorative treatment, amalgam restoration decreased and resin restoration increased. 4. Frequency of sedation decreased in 2006, 2007 and increased in 2008. 5. Frequency of general anesthesia increased up to 2008. 소아치과 전문 지역 의원의 증가, 소아치과에 대한 보호자의 인식 변화, 치의학의 발전, 소아치과 진료 범주의 확대 등에 의해 최근 소아치과의 진료 양상이 크게 변화하고 있다. 이러한 변화 양상을 파악하고 앞으로의 대학병원 소아치과 및 소아치과 전문 지역 의원의 진료분담에 대한 방향성을 제시하고자 본 연구를 시행하였다. 2001년 1월 1일부터 2008년 12월 31일까지 서울대학교치과병원 소아치과에 내원한 모든 초진 및 재진 환자를 대상으로, 서울대학교치과병원 종합의료정보시스템(OCS)을 통하여 1년간의 외래환자집계 및 수가코드별 진료 행위 건수 및 수익 분포를 조사하여 다음과 같은 결과를 얻었다. 1. 내원환자의 수는 2001년부터 2005년까지 대체로 증가하다 2006년에 감소하였으며 그 이후에는 2006년도와 별다른 변화가 없었다. 2. 2001년에는 예방치료가 6.84%, 수복치료가 49.96%, 치수치료가 9.64%, 외과치료가 10.78%, 교정치료가 22.78%를 차지하였으나, 2008년에는 예방치료가 15.40%, 수복치료가 28.97%, 치수치료가 3.28%, 외과치료가 14.83%, 교정치료가 37.52%를 차지하여 수복치료와 치수치료의 비율은 감소하고 예방치료와 교정치료는 증가하였다. 3. 수복치료의 비율에 있어서 아말감과 글라스 아이오노머 수복 및 기성금관수복의 비율은 감소하였고, 레진의 비율은 증가하였다. 4. 진정요법하 치과치료는 2005년까지 증가하다 2006년, 2007년에는 감소하였고, 2008년에 다시 증가하였다. 5. 전신마취하 치과치료는 2001년부터 2008년까지 꾸준히 증가하였다.

어·패류에서 납의 조직 침투율 및 잔류납의 효과적인 분석법에 관한 연구

이진훈,오세진,이진숙,김유나 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

Polaron Hopping in the K_(2)O · B_(2)O_(3) · V_(2)O_(5) Glasses

차유정,강재필,홍성덕,김복수,김영훈,서용문,송승기 명지대학교 자연과학연구소 2005 자연과학논문집 Vol.24 No.-

K_(2)O · B_(2)O_(3) · V_(2)O_(5) 계 유리의 조성비에 따른 밀도, 굴절율, 전이온도, 천이금속이온비율 등과 직류 전기 성질을 100~450K 온도영역에서 연구하였다. 실험결과를 비정질 반도체에 관한 전기전도에 대한 이론 모델을 이용하여 분석하였다. 고온 영역에서는 직류전도도의 온도 의존성을 Mott의 포논-도움껑충뛰기 전도모델로 분석할 수 있었다. 활성화에너지는 0.28~0.33 eV로 시료내 V_(2)O_(5) 의 양이 증가할수록 감소하는 경향을 보인다. 저온 영역에서 직류 전기전도도는 Mott의 가변거리 껑충뛰기(variable-range hopping)전기전도도와 잘 일치하며, N(E_(F))값은 국지화 상태에서와 같다. The dc conductivity of K_(2)O-B_(2)O_(3)-V_(2)O_(5) glasses has been investigated in the temperature range 100~450K and physical parameters such as density, refractive indices, glass transition temperature and fraction of reduced transition-metal ion, were observed for various compositions. The experimental results are analyzed with reference to theoretical models proposed for electrical conduction in amorphous semiconductors. The analysis shows that at high temperature region the temperature dependence of the dc conductivity is consistent with Mott's model of phonon-assisted hopping of small polarons. The values of activation energy are 0.28~0.33 eV and decrease with increasing V_(2)O_(5) content in the sample. The lowtemperature dc conductivity data can be well fitted to Mott's variable-range hopping conductivity and the values of N(E_(F)) are also reasonable for localized states.

혈액 및 비장조직에서 일산화탄소 헤모글로빈 농도에 관한 연구 : A Comparison of CO-Hb in Blood and Spleen Specimens

이진훈,오세진,김유나,이진숙,유재훈,장성길 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-

이륜차 교통사고의 법의감정

김유훈 內務部 國立科學搜査硏究所 2002 國立 科學 搜査 硏究所 年報 Vol.34 No.-

A Passage to India에 관한 小考

김여훈 동의공업대학 1999 論文集 Vol.25 No.1

The structure of A Passage to India is three-fold, It is composed of three dominant metaphors: 'Mosque', 'Caves' and 'Temple. These three sections of the novel have their narrative as well as symbolic substances and meanings. The episodes in 'Mosque', bring peoples of different cultures and religions together, and they also alienate them from each other. The failure of the Bridge party is an ironic comment on the attempt to forge a link of friendship between east and west, India and Anglo-India, the ruler and the ruled. The party does not go beyond formal civilities and surface graces. The visit to the Marabar Caves is the central episode in the novel. The episodes in the Caves also symbolize not only a spiritual and physical breakdown but the barrier between east and west. After returning from the Marabar Caves to Chandrapore. Aziz is arrested on a charge of having tried to rape Adela in the Caves. The atmosphere in Chandrapore suddenly becomes tense. The trial of Aziz creates social and political tensions in the town and also distrust between Indian and English people. Although E. M. Forster tries to show the harmony between India and Britain in the novel, the last scene in the Mau jungles seems to darken the personal relationship between Aziz and Fielding unless India becomes a free country and all Englishmen as rulers leave India for good.

初等學敎의 建築的 特性에 관한 硏究

나상훈,김유곤 서강대학 지역발전연구소 1999 지역발전연구 : 서강전문대 Vol.5 No.2

This study based upon theoretical contemplation has tried to analyze the composition of open school spaces of the elementary schools in Kwang-ju whose designs have been chosen by competition since 1990, and thereby to suggest both its problems and basic materials necessary for the construction of new type of school buildings. The results of this study are as follows: The school buildings are usually separated and arranged according to higher classes and lower classes, special classrooms and general classrooms, and classrooms and control offices. But there are usually pilots on the first floor, the other floors are connected directly and often there are arranged special rooms in connecting passages. School buildings, a gymnasium, and a play ground are common factors in every school. And there are small play grounds for lower class students or kindergarteners who have some difficulty in using play grounds, and these spaces are separated and arranged for higher class students, lower class students, and kindergarteners each. In many schools, the size of a unit classroom is the standard size of 67.5㎡. But it seems that a size of each unit classroom must be enlarged to utilize small spaces and to provide some spaces for open education.

감전사에 대한 법의학적 고찰

김유훈 內務部 國立科學搜査硏究所 2001 國立 科學 搜査 硏究所 年報 Vol.33 No.-