Serial values for hematologic and biochemical analysis after myocardial infarction in rats

( Mi Jin Lee ),( Hyun Jin Tae ),( Ying Hua Li ),( Do Hyeon Yu ),( In Ae Han ),( Seok Won Lee ),( Dong Choon Ahn ),( In Shik Kim ),( Jin Ho Park ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction(MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hema-tologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and bioche-mical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery(LMDA) of the left coronary artery was ligated during the progression(day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group(n=24, thoracotomy without LMDA ligation) and the experimental(MI) group(n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell(RBCs), hemoglobin and hematocrit(HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, choles-terol, total creatine kinase(CK) and isoenzyme MB, and lactate dehydrogenase(LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Serial values for hematologic and biochemical analysis after myocardial infarction in rats

Lee, Mi-Jin,Tae, Hyun-Jin,Li, Ying-Hua,Yu, Do-Hyeon,Han, In-Ae,Lee, Seok-Won,Ahn, Dong-Choon,Kim, In-Shik,Park, Jin-Ho The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction (MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hematologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and biochemical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery (LMDA) of the left coronary artery was ligated during the progression (day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group (n=24, thoracotomy without LMDA ligation) and the experimental (MI) group (n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell (RBCs), hemoglobin and hematocrit (HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, cholesterol, total creatine kinase (CK) and isoenzyme MB, and lactate dehydrogenase (LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

역행성 심정지 도관 삽입을 위한 술 중 경식도심초음파의 유용성

유미숙;최원주;김정원;박장수;김경태;김지연;어상일 인제대학교 2011 仁濟醫學 Vol.32 No.-

Background: Retrograde cardioplegia catheter (RCC) insertion is one of methods for administration of cardioplegics during cardiopulmonary bypass (CPB). However its success rate depends on only surgeon's experience. We thought the transesophageal echocardiography (TEE) can guide the R CC insertion, because TEE is commonly used for monitoring status of heart during cardiac surgery. This study was designed to evaluate the RC C insertion time by TEE guidance versus conventional method without T EE guidance. Methods: Sixty patients who were scheduled to undergo elective coronary artery bypass graft (CABG) surgery under general anesthesia were divided into groups as follows: group T [RCC insertion into coronary sinus (CS) by TEE guidance, n=30] and group C [RCC insertion into CS by conventional method, n=30]. We recorded the time of insertion of RCC in to CS in two groups. RCC insertion was done by TEE guidance in case that time was over two minutes by conventional methods in group C. Results: The overall time of RCC insertion into CS was 63.8 ± 23.6 seconds in the Group T and 102.3 ±55.7 seconds in the Group C, respectively (p=0.001). There were no complications during RCC into CS by T EE guidance. Conclusions: TEE guidance for RCC insertion into CS is shorten the time of insertion.

신규간호사와 프리셉터가 인지하는 프리셉터의 교수효율성 비교

김지양,김영선,김춘실,박현숙,신미영,윤연숙,조유숙,박미미,유문숙 한국간호과학회 간호행정학회 2009 간호행정학회지 Vol.15 No.2

Objective: The purpose of this study was to compare preceptors and new graduate nurses on their perception of preceptor teaching effectiveness. Methods: The participants were 90 new nurses and 90 preceptors who worked in A medical center. The data were collected from July 1 to September 30, 2008. A cross-sectional descriptive survey was done using a structured questionnaire. Results: New nurses' perception (4.07±.44) of the preceptor teaching effectiveness was significantly higher than perception of the preceptors (3.57±.37). Fifty five percent of new graduate nurses reported a lack of coherence in the preceptor's practice guidelines. For 5 causal factors classified on the teaching effectiveness, the factor of 'Professional knowledge and ability' showed the highest score, but 'Interpersonal and communication skill' got lowest score for both group. Preceptors responded that they did not have enough time to teach well because of their heavy workloads. Conclusions: These results suggest that the preceptors need appropriate compensation and education opportunities, and new graduate nurses need consistent education by the teaching professionals. Therefore, it is important to give preceptors full charge of the preceptorship. Also, it will be necessary to develop education programs to enhance interpersonal and communication skill for preceptors and new nurses.

제 1형 신경섬유종증 환자에서 발생한 위장관 간질종양 1예

원경준,이준,변유미,조민근,한경택,김원,홍란,임성철,김경종,김영대,박찬국,김만우 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Gastrointestinal stromal tumor (GIST) is the most common non-epithelial neoplasm arising in the gastrointestinal tract, but this tumor is rarely seen in association with type l neurofibromatosis (NFl) Generally, the majority of GISTs are isolated neoplasm and they are sporadically found in the stomach, but the GISTs in NFl patients are usually multiple and usually found in the small intestine. We report a case of multiple GISTs in the jejunum of a 63-year-old woman diagnosed as NFl accompanied by complication of gastrointestinal bleeding, In this case, Patient had multiple cafe-au lait spots and neurofibroma on skin and had freckling on axilla and groin, and then, we made a diagnosis of NFl. Gastrointestinal bleeding is controlled by resection of multiple GISTs. Generally, only 3-5% of all gastrointestinal bleeding comes from the small bowel. Causes of small intestinal bleeding are angiodysplasia, neoplasm, NSAIDs induced ulcer etc. but, If patients have NFl accompanied by complication of gastrointestinal bleeding, GISTs on small bowel must be considered.

한국어판 표출감정평가척도의 신뢰도와 타당도

김진훈,김대호,강유미,박성혁,이창훈,장동원 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.2

Objectives : The present study was designed to assess the reliability and validity of Korean version ofFamily Questionnaire (FQ-K), a briefquestionnaire measuring expressed emotion (EE). Methods : A sample of 84 first-order relatives ofschizophrenics completed the FQ-K and the data were analyzed for intemal consistency and factor structure. Additionally non-random subset of participants (n=34) refilled FQ-K after two weeks for test-retest reliability and naturalistic follow-up data of 36 were obtained for any re-hospitalization events to assess predictive validity. Results : Intemal consistency of FQ-K was good (Cronbach's alpha=.819) and pimcipal component factor analysis revealedthe two-factor structure (criticism and emotional overinvolvement) identical to previously reported for the FQ. The FQ-K also showed reasonable test-retest reliability and excellent predictive validity was indicated in that outcome of re-hospitalization was superior for low expressed emotion patients at six-month naturalistic follow-up. Conclusion : The Korean version of FQ Proved to be a reliable and valid instrument measuring expressed emotion in relatives of Korean patients with schizophrenia.

Cisplatin 병용화학요법시 발생한 오심, 구토에 대한 Ondansetron과 Metoclopramide의 효과에 대한 비교

김미자,국기용,김태원,박유환,김완중,허경,정춘해,홍순표 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

Inspite of possible effects for emesis following chemotherapy including cisplatin, nausea and vomiting are the most unpleasant side effects of cancer chemotherapy. None of the currently available antiemetic agents is entirely effective preventing emesis. Ondansetron, a 5-HT(5-hydroxytryptamine)_(3) receptor antagonist, has been shown to be effective antiemetic agent in the control of cisplatin induced emesis. Twenty solid tumor patients who are scheduled to receive cisplatin containing combination chemotherapy participated in a prospectively randomized study to compare the antiemetic efficacy and safety of ondansetron and metoclopramide. Ondansetron was given in dose of 8mg intravenous 30min before cisplatin and then 4hours and 8hours after cisplatin on day 1, and Ⅰ.Ⅴ. every 8hours from day 2 to 5. In the metoclopramide group, metoclopramide was given in dose of 2 ㎎/㎏ intravenous every 8hours from day 1 to 5. The control of emesis was graded in the following way : complete response, no vomiting : major response, 1-2 emetic episodes : minor response, 3-5 emetic episodes : failure, above 5 emetic episodes. Complete or major response of emesis was achieved 10 of 12(83.2%) patients receiving ondansetron and in 5 of 8(62.5%) patients receiving metoclopramide, but effectiveness was not significantly different. Ondansetron was at least as effective as metoclopramide therapy in controlling cisplatin induced emesis. Side effects in ondansetron was less than in Metoclopramide.

재조합 대장균의 고농도 유가식 배양에 의한 인체 Granulocyte-Colony Stimulating Factor 생산

김범수,유미진 建陽大學校 1999 建陽論叢 Vol.- No.7

Fed-batch fermentation of recombinant Escherichia coli harboring the gene encoding mature human granulocyte-colony stimulating factor (hG-CSF) fused to the gene for the pectate lyase B (pelB) signal peptide of Erwinia caratovora was performed. The fusion protein, pelB-hG-CSF, was produced at a concentration of ca. 1.7 g/L in an inclusion body form.

중금속 이온 존재 하에서의 아스코르브산 자동산화반응에 대한 단백질의 영향

김미옥,유리나 울산대학교 2002 생활과학논문집 Vol.4 No.1

Effects of superoxide dismutase(SOD), catalase, and such other proteins as bovine serum albumin(BSA), ovalbumin, lysozyme, and γ-globulin on the autoxidation rates of L-ascorbic acid(AsA) in the presence of heavy metal ions in aqueous solution and buffer solution were studied. AsA was dissolved in a ultra-refined water and 0.07 M potassium phosphate buffer (pH 7.4, treated Chelex-100) at a concentration of 50μM and 5 μM Fe(Ⅲ) or 0.1 μM Cu(Ⅱ) was added, and a oxygen gas was bubbled through the solution at a flow rate of 200 ml/min at 35℃. The amount of remaining AsA in the reaction mixture was determined by using a UV spectrophotometer(at 265 nm). It was confirmed that the ratio of remaining AsA was significantly larger in the presence of SOD, catalase, BSA, ovalbumin, lysozyme, and γ-globulin than in the absence of proteins. It was suggested that the non-enzymatic effects of SOD, catalase and some other proteins might be involves in the stabilization of AsA.

콜로이드 골드 나노입자의 단백질 수송성 평가법

김미영,노상명,김정목,최한곤,김정애,오유경 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.6

Colloidal gold nanoparticles might be of use as nano scale delivery systems of various therapeutic materials in the firture. Recent studies have reported the feasibility of colloidal gold nanoparticles as gene delivery systems or protein delivery systems. In this study, we aimed to develop a short-step method useful for screening the optimal coating conditions of colloidal gold nanoparticles with proteins. We observed that colloidal gold nanoparticles have properties of changing its unique color when they were exposed to NaCl solution. Taking advantage of the color changing properties of colloidal gold nano-particles, we applied the color testing method of colloidal gold nanoparticles solutions for evaluating the protein coating nature. Using bovine senim albumin as a model protein, we tested the protein coating of colloidal gold nanoparticles via the color change upon NaCl addition. The optimal coating concentration and coating conditions of colloidal gold nanoparticles with bovine serum albumin were fixed using the color testing methods. We suggest that the color testing method might be applied to optimize the coating condition of colloidal gold nanoparticles with other therapeutic proteins.